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Measurements of optical properties of high-density media
Abstract
Measurements of optical properties (scattering coefficient mu(s), absorption coefficient mu(a), reduced scattering coefficient mu(s)', and asymmetry factor g) have been carried out up to a volume particle concentration of rho = 0.227. The results for mu(s) and mu(s)' show significant deviations from the linear dependence on rho as expected when the independent scattering assumption is fulfilled. The asymmetry factor also changed significantly. In contrast, the dependence of mu(a) remained linear even at the largest concentration investigated. The simple linear dependence of absorption on the chromophore concentration expected from the independent scattering assumption is thus applicable also to spectroscopic measurements of dense media. A comparison with an approximate theoretical model based on the Foldy-Twersky equation is also reported. The model provides a good description of the dependence of mu(s) on particle concentration.
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“However, the OD alone is an unreliable metric and is only proportionately accurate to cell titers to about an OD of 0.1.”
From Estimating microbial population data from optical density (2022) · cites this paper as [5]
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References 20 · Cited by 141
Metadata
- Authors
- Zaccanti, G., Del Bianco, S., & Martelli, F.
- Affiliations
- Istituto Nazionale per la Fisica della Materia (IT)
- ORCID
- Martelli, Fabrizio
- Venue
- Applied Optics
- Publisher
- Optica Publishing Group
- Published
- 2003-07-01
- Volume
- 42
- Issue
- 19
- Pages
- 4023
- ISSN
- 0003-6935 (ISSN-L)
- 1539-4522
- OpenAlex
- W1999283429
- Cited by
- 141
- References
- 20
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 7 · 2023: 1 · 2022: 6 · 2021: 6 · 2020: 8 · 2019: 4 · 2018: 9 · 2017: 11 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 9 · 2012: 8
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- doi.org/10.1364/OA_License_v1#VOR (vor)
- opg.optica.org/policies/opg-tdm-policy.json (tdm)
- Locations
- Applied Optics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Random lasers and scattering media
- Physical Sciences › Engineering › Computational Mechanics › Radiative Heat Transfer Studies
- Keywords
- scattering coefficient, absorption coefficient, reduced scattering coefficient, asymmetry factor, particle concentration dependence, chromophore absorption, turbid media
- MeSH terms
- Computer Simulation, Densitometry, Fat Emulsions, Intravenous, Lasers, Microspheres, Models, Theoretical, Particle Size, Refractometry, Scattering, Radiation, Sensitivity and Specificity, Reproducibility of Results, Optics and Photonics
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- Zaccanti, G., Del Bianco, S., & Martelli, F. (2003). Measurements of optical properties of high-density media. Applied Optics. https://doi.org/10.1364/ao.42.004023
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