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Cited by 282

The DOI matches its Crossref record; 363 of the 363 references it cites were found in a registry or an index.

Optical tweezers — from calibration to applications: a tutorial

Open access
Gieseler, Jan, Gomez-Solano, Juan Ruben, Magazzù, Alessandro, Pérez Castillo, Isaac, Pérez García, Laura, Gironella-Torrent, Marta, Viader-Godoy, Xavier, Ritort, Felix, Pesce, Giuseppe, Arzola, Alejandro V., Volke-Sepúlveda, Karen, Volpe, Giovanni·2021·Advances in Optics and Photonics

Abstract

Since their invention in 1986 by Arthur Ashkin and colleagues, optical tweezers have become an essential tool in several fields of physics, spectroscopy, biology, nanotechnology, and thermodynamics. In this tutorial, we provide a primer on how to calibrate optical tweezers and how to use them for advanced applications. After a brief general introduction on optical tweezers, we focus on describing and comparing the various available calibration techniques. Then, we discuss some cutting-edge applications of optical tweezers in a liquid medium, namely, to study single-molecule and single-cell mechanics, microrheology, colloidal interactions, statistical physics, and transport phenomena. Finally, we consider optical tweezers in vacuum, where the absence of a viscous medium offers vastly different dynamics and presents new challenges. We conclude with some perspectives for the field and the future applications of optical tweezers. This tutorial provides both a step-by-step guide ideal for non-specialists entering the field and a comprehensive manual of advanced techniques useful for expert practitioners. All of the examples are complemented by the sample data and software necessary to reproduce them.

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Metadata

Authors
Gieseler, J., Gomez-Solano, J. R., Magazzù, A., Pérez Castillo, I., Pérez García, L., Gironella-Torrent, M., Viader-Godoy, X., Ritort, F., Pesce, G., Arzola, A. V., Volke-Sepúlveda, K., & Volpe, G.
Venue
Advances in Optics and Photonics
Published
2021-03-09
Volume
13
Issue
1
Pages
74
ISSN
1943-8206 (ISSN-L)
OpenAlex
W3107944508
Cited by
282
References
363
FWCI
12.23
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Optical tweezers — from calibration to applications: a tutorial. https://app.cochat.ai/papers/10.1364/aop.394888
The paper
Gieseler, J., Gomez-Solano, J. R., Magazzù, A., Pérez Castillo, I., Pérez García, L., Gironella-Torrent, M., Viader-Godoy, X., Ritort, F., Pesce, G., Arzola, A. V., Volke-Sepúlveda, K., & Volpe, G. (2021). Optical tweezers — from calibration to applications: a tutorial. Advances in Optics and Photonics. https://doi.org/10.1364/aop.394888

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