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Multiplexed coded illumination for Fourier Ptychography with an LED array microscope

Open access
Tian, Lei, Li, Xiao, Ramchandran, Kannan, Waller, Laura·2014·Biomedical Optics Express

Abstract

Fourier Ptychography is a new computational microscopy technique that achieves gigapixel images with both wide field of view and high resolution in both phase and amplitude. The hardware setup involves a simple replacement of the microscope's illumination unit with a programmable LED array, allowing one to flexibly pattern illumination angles without any moving parts. In previous work, a series of low-resolution images was taken by sequentially turning on each single LED in the array, and the data were then combined to recover a bandwidth much higher than the one allowed by the original imaging system. Here, we demonstrate a multiplexed illumination strategy in which multiple randomly selected LEDs are turned on for each image. Since each LED corresponds to a different area of Fourier space, the total number of images can be significantly reduced, without sacrificing image quality. We demonstrate this method experimentally in a modified commercial microscope. Compared to sequential scanning, our multiplexed strategy achieves similar results with approximately an order of magnitude reduction in both acquisition time and data capture requirements.

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Metadata

Authors
Tian, L., Li, X., Ramchandran, K., & Waller, L.
Venue
Biomedical Optics Express
Published
2014-06-19
Volume
5
Issue
7
Pages
2376
ISSN
2156-7085 (ISSN-L)
OpenAlex
W1986579450
Cited by
584
References
13
FWCI
43.26
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Multiplexed coded illumination for Fourier Ptychography with an LED array microscope. https://app.cochat.ai/papers/10.1364/boe.5.002376
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Tian, L., Li, X., Ramchandran, K., & Waller, L. (2014). Multiplexed coded illumination for Fourier Ptychography with an LED array microscope. Biomedical Optics Express. https://doi.org/10.1364/boe.5.002376

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