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Quantitative imaging of complex samples by spiral phase contrast microscopy
Open accessAbstract
Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell.
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Cited by
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Willner, A. E., Huang, H., Yan, Y., Ren, Y., Ahmed, N., Xie, G., Bao, C., Li, L., Cao, Y., Zhao, Z., et al. (2015) · Advances in Optics and Photonics · doi:10.1364/aop.7.000066 · Cited by 1,943
Zhao, H., Quan, B., Wang, X., Gu, C., Li, J., & Zhang, Y. (2018) · ACS Photonics · doi:10.1021/acsphotonics.7b01149 · Cited by 181
Metadata
- Authors
- Bernet, S., Jesacher, A., Fürhapter, S., Maurer, C., & Ritsch-Marte, M.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2006
- Volume
- 14
- Issue
- 9
- Pages
- 3792
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W1981106629
- Cited by
- 276
- References
- 0
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- gold
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- Bernet, S., Jesacher, A., Fürhapter, S., Maurer, C., & Ritsch-Marte, M. (2006). Quantitative imaging of complex samples by spiral phase contrast microscopy. Optics Express. https://doi.org/10.1364/oe.14.003792
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