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Optically sectioned imaging by oblique plane microscopy
Open accessAbstract
This paper describes a new optically sectioning microscopy technique based on oblique selective plane illumination combined with oblique imaging. This method differs from previous selective plane illumination techniques as the same high numerical aperture lens is used to both illuminate and image the specimen. Initial results obtained using fluorescent pollen grains are presented, together with a measurement of the resolution of the system and an analysis of the potential performance of future systems. Since only the plane of the specimen that is being imaged is illuminated, this technique is particularly suited to time-lapse 3-D imaging of sensitive biological systems where photobleaching and phototoxicity must be kept to a minimum, and it could also be applied to image microfluidic technology for lab-on-a-chip, cytometry and other applications.
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Cited by
Selective plane illumination microscopy techniques in developmental biology
Huisken, J., & Stainier, D. Y. R. (2009) · Development · doi:10.1242/dev.022426 · Cited by 608
Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0
Voleti, V., Patel, K. B., Li, W., Perez Campos, C., Bharadwaj, S., Yu, H., Ford, C., Casper, M. J., Yan, R. W., Liang, W., et al. (2019) · Nature Methods · doi:10.1038/s41592-019-0579-4 · Cited by 320
Light-sheet microscopy: a tutorial
Olarte, O. E., Andilla, J., Gualda, E. J., & Loza-Alvarez, P. (2018) · Advances in Optics and Photonics · doi:10.1364/aop.10.000111 · Cited by 298
In vivo NIR-II structured-illumination light-sheet microscopy
Wang, F., Ma, Z., Zhong, Y., Salazar, F., Xu, C., Ren, F., Qu, L., Wu, A. M., & Dai, H. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2023888118 · Cited by 85
Evolution and impact of high content imaging.
Way, G. P., Sailem, H., Shave, S., Kasprowicz, R., & Carragher, N. O. (2023) · SLAS Discovery · doi:10.1016/j.slasd.2023.08.009 · Cited by 67
Metadata
- Authors
- Dunsby, C.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2008-11-24
- Volume
- 16
- Issue
- 25
- Pages
- 20306
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2057132085
- Cited by
- 348
- References
- 0
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
