The DOI matches its Crossref record.
Single-molecule detection by two-photon-excited fluorescence
Abstract
We present a technique for observing single fluorophore molecules in solution. A mode-locked laser beam is focused into the solution, thereby defining a two-photon excitation volume localized in three dimensions. Molecules diffusing into and out of this volume produce fluorescence bursts, which are detected with a high signal-to-background ratio. The theoretical foundations for the technique are laid out, including the attendant fluorescence rate distribution, and agree with experimental results obtained for Rhodamine B molecules in water.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations
Levene, M. J., Korlach, J., Turner, S. W., Foquet, M., Craighead, H. G., & Webb, W. W. (2003) · Science · doi:10.1126/science.1079700 · Cited by 1,378
Self-citationPhotolysis of Caged Calcium in Femtoliter Volumes Using Two-Photon Excitation
Brown, E. B., Shear, J. B., Adams, S. R., Tsien, R. Y., & Webb, W. W. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77217-6 · Cited by 181
Self-citation
Metadata
- Authors
- Mertz, J., Xu, C., & Webb, W. W.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 1995-12-15
- Volume
- 20
- Issue
- 24
- Pages
- 2532
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W1998535083
- Cited by
- 225
- References
- 0
- FWCI
- 11.03
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Single-molecule detection by two-photon-excited fluorescence. https://app.cochat.ai/papers/10.1364/ol.20.002532
- The paper
- Mertz, J., Xu, C., & Webb, W. W. (1995). Single-molecule detection by two-photon-excited fluorescence. Optics Letters. https://doi.org/10.1364/ol.20.002532
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
