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Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration

Hashimoto, Mamoru, Araki, Tsutomu, Kawata, Satoshi·2000·Optics Letters

Abstract

We have developed a new coherent anti-Stokes Raman scattering (CARS) microscopy system with a collinear configuration for use in the fingerprint region. The system consists of a picosecond laser system and a transmission-type laser scanning microscope without a pinhole in front of the detector. The observable Raman-shift region is 900-1750 cm(-1), the spectral resolution is 30cm(-1), and the spatial resolution is smaller than 1 mum in the lateral direction and 3.2 mum in the depth direction, with objectives with a numerical aperture of 0.65. CARS spectra and images of polystyrene beads are demonstrated, and CARS imaging of a viable yeast cell is attempted.

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Metadata

Authors
Hashimoto, M., Araki, T., & Kawata, S.
Venue
Optics Letters
Published
2000-12-15
Volume
25
Issue
24
Pages
1768
ISSN
  • 0146-9592 (ISSN-L)
  • 1539-4794
OpenAlex
W2011265333
Cited by
203
References
0
FWCI
5.47
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration. https://app.cochat.ai/papers/10.1364/ol.25.001768
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Hashimoto, M., Araki, T., & Kawata, S. (2000). Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration. Optics Letters. https://doi.org/10.1364/ol.25.001768

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