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Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration
Abstract
We have developed a new coherent anti-Stokes Raman scattering (CARS) microscopy system with a collinear configuration for use in the fingerprint region. The system consists of a picosecond laser system and a transmission-type laser scanning microscope without a pinhole in front of the detector. The observable Raman-shift region is 900-1750 cm(-1), the spectral resolution is 30cm(-1), and the spatial resolution is smaller than 1 mum in the lateral direction and 3.2 mum in the depth direction, with objectives with a numerical aperture of 0.65. CARS spectra and images of polystyrene beads are demonstrated, and CARS imaging of a viable yeast cell is attempted.
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Citations
Cited by
Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications
Cheng, J.-X., & Xie, X. S. (2004) · The Journal of Physical Chemistry B · doi:10.1021/jp035693v · Cited by 963
Chemically sensitive bioimaging with coherent Raman scattering
Camp Jr, C. H., & Cicerone, M. T. (2015) · Nature Photonics · doi:10.1038/nphoton.2015.60 · Cited by 472
Tip-Enhanced Coherent Anti-Stokes Raman Scattering for Vibrational Nanoimaging
Ichimura, T., Hayazawa, N., Hashimoto, M., Inouye, Y., & Kawata, S. (2004) · Physical Review Letters · doi:10.1103/physrevlett.92.220801 · Cited by 405
Self-citationSpectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses
Hellerer, T., Enejder, A. M. K., & Zumbusch, A. (2004) · Applied Physics Letters · doi:10.1063/1.1768312 · Cited by 384
Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy
Cheng, J.-X., Volkmer, A., & Xie, X. S. (2002) · Journal of the Optical Society of America B · doi:10.1364/josab.19.001363 · Cited by 377
Metadata
- Authors
- Hashimoto, M., Araki, T., & Kawata, S.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 2000-12-15
- Volume
- 25
- Issue
- 24
- Pages
- 1768
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W2011265333
- Cited by
- 203
- References
- 0
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hashimoto, M., Araki, T., & Kawata, S. (2000). Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration. Optics Letters. https://doi.org/10.1364/ol.25.001768
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