Quantification of lamellar orientation in corneal collagen using second harmonic generation images
Open accessAbstract
Second harmonic generation (SHG) is a well-established optical modality widely used in biomedical optics to image collagen based tissues. The coherent signal of the forward direction SHG produces a high resolution image that can resolve individual fibers (groups of fibrils). In highly ordered collagen lamellae, such as in the corneal stroma, it is important to determine the orientation of the fibers as they contribute significantly to the biomechanics of the tissue. However, due to the crimped structure of the fibers, it is challenging to robustly determine their orientation using an independent computational method, compared to the straight fibers problem. Previous work in the field used the polarization of the fundamental or other techniques involving a more manual selection of the orientation, in order to differentiate between various directions in corneal structures. Yet those lack accuracy and independency. We present a robust independent technique to determine the orientation of the fibers in the corneal structure. The experimental results presented here, taken from different lamellae, demonstrate strongly the correct orientation.
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Second harmonic generation imaging can provide qualitative and quantitative information about lamellar organisation within the cornea and has the potential to be used in vivo.
“Over the last decade there has been an upsurge in the use of non-linear microscopy, in particular second harmonic generation imaging, to provide qualitative (Han et al., 2005, Morishige et al., 2006, Teng et al., 2006, Bueno et al., 2011) and quantitative (Mega et al., 2012, Tuer et al., 2012, Ghazaryan et al., 2013) information about the lamellar organisation within the cornea, a technique that,”
From Corneal structure and transparency (2015) · cites this paper as Mega et al., 2012
The paper’s Abstract describes a method for quantifying collagen-lamellar orientation in corneal SHG images, supporting the quantitative part for which Mega et al. is cited. The experiments used a bovine cornea placed on a microscope cover slip; our copy does not establish the claimed potential for in vivo use. In the printed sentence, the qualitative citations are to other papers. (LLM, conf=0.86)
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Research works printed but not linked: 8
- [3]Psilodimitrakopoulos S Petegnief V Soria G Amat-Roldan I Artigas D Planas AM Loza-Alvarez P Opt Express 17 14418 2009 19654849
- [4]Williams RM Zipfel WR Webb WW Biophys J 88 1377 2005 15533922 PMC1305140
- [5]Rao RA Mehta MR Toussaint KC Opt Express 17 14534 2009 19687932
- [6]Rao RAR Mehta MR Leithem S Toussaint JKC Opt Lett 34 3779 2009 20016611
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Cited by
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Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
- Authors
- Mega, Y., Robitaille, M., Zareian, R., McLean, J., Ruberti, J., & DiMarzio, C.
- Affiliations
- Northeastern University (US)
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 2012-08-03
- Volume
- 37
- Issue
- 16
- Pages
- 3312
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- PMC
- PMC3784649
- OpenAlex
- W2008719519
- Cited by
- 52
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2022: 5 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 6 · 2016: 6 · 2015: 9 · 2014: 1 · 2013: 4
- FWCI
- 2.05
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Optics Letters (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Eye Institute (R01EY015500)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal surgery and disorders
- Physical Sciences › Engineering › Biomedical Engineering › Optical Polarization and Ellipsometry
- Keywords
- second harmonic generation, corneal collagen, collagen fiber orientation, corneal stroma, lamellar orientation, fiber crimp
- MeSH terms
- Animals, Cattle, Collagen, Cornea, Microscopy, Fluorescence, Molecular Conformation, Molecular Imaging
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