Second harmonic generation imaging of collagen fibrils in cornea and sclera
Open accessAbstract
Collagen, as the most abundant protein in the human body, determines the unique physiological and optical properties of the connective tissues including cornea and sclera. The ultrastructure of collagen, which conventionally can only be resolved by electron microscopy, now can be probed by optical second harmonic generation (SHG) imaging. SHG imaging revealed that corneal collagen fibrils are regularly packed as a polycrystalline lattice, accounting for the transparency of cornea. In contrast, scleral fibrils possess inhomogeneous, tubelike structures with thin hard shells, maintaining the high stiffness and elasticity of the sclera.
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Second harmonic generation imaging can provide qualitative and quantitative information about lamellar organisation within the cornea and has the potential to be used in vivo.
“Over the last decade there has been an upsurge in the use of non-linear microscopy, in particular second harmonic generation imaging, to provide qualitative (Han et al., 2005, Morishige et al., 2006, Teng et al., 2006, Bueno et al., 2011) and quantitative (Mega et al., 2012, Tuer et al., 2012, Ghazaryan et al., 2013) information about the lamellar organisation within the cornea, a technique that,”
From Corneal structure and transparency (2015) · cites this paper as Han et al., 2005
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Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure
Chen, X., Nadiarynkh, O., Plotnikov, S., & Campagnola, P. J. (2012) · Nature Protocols · doi:10.1038/nprot.2012.009 · Cited by 985 · see citing works
Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
Lu, J., Surya, J. B., Liu, X., Bruch, A. W., Gong, Z., Xu, Y., & Tang, H. X. (2019) · Optica · doi:10.1364/optica.6.001455 · Cited by 445 · see citing works
- Authors
- Han, M., Giese, G., & Bille, J. F.
- Affiliations
- Heidelberg University (DE)
- Max Planck Society (DE)
- ORCID
- Han, Meng
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2005
- Volume
- 13
- Issue
- 15
- Pages
- 5791
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W1974350661
- Cited by
- 293
- References
- 0
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 7 · 2023: 8 · 2022: 10 · 2021: 7 · 2020: 10 · 2019: 12 · 2018: 16 · 2017: 17 · 2016: 22 · 2015: 23 · 2014: 18 · 2013: 20 · 2012: 24
- FWCI
- 6.87
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Optics Express (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Bundesministerium für Bildung und Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal surgery and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- collagen fibrils, second harmonic generation microscopy, cornea, sclera, collagen ultrastructure, corneal transparency, scleral stiffness, connective tissue biomechanics
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