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On the Spectral Subtraction of Water from the FT-IR Spectra of Aqueous Solutions of Proteins
Abstract
A method is described for the quantitative subtraction of water from the transmission infrared spectra of aqueous solutions of proteins. The 2125-cm−1 association band of water is used as an internal intensity standard, and the scaling factor is determined with the use of a second-order least-squares fit. This method eliminates the user bias encountered with interactive methods and takes into account small baseline variations due to instrument drift. Statistical analysis of the results obtained demonstrates that at 10% w/w protein concentration, the error is of the order of 2% in the region of the amide I and II bands.
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Citations
Cited by
Venyaminov, S. Y., & Prendergast, F. G. (1997) · Analytical Biochemistry · doi:10.1006/abio.1997.2136 · Cited by 408
Investigation of ciprofloxacin penetration into Pseudomonas aeruginosa biofilms
Suci, P. A., Mittelman, M. W., Yu, F. P., & Geesey, G. G. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.9.2125 · Cited by 357
Bremer, P. J., & Geesey, G. G. (1991) · Biofouling · doi:10.1080/08927019109378165 · Cited by 96
Boulet-Audet, M., Buffeteau, T., Boudreault, S., Daugey, N., & Pézolet, M. (2010) · The Journal of Physical Chemistry B · doi:10.1021/jp101763y · Cited by 62
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Metadata
- Authors
- Dousseau, F., Therrien, M., & Pézolet, M.
- Venue
- Applied Spectroscopy
- Publisher
- SAGE Publications
- Published
- 1989-03
- Volume
- 43
- Issue
- 3
- Pages
- 538-542
- ISSN
- 0003-7028 (ISSN-L)
- 1943-3530
- OpenAlex
- W1988918623
- Cited by
- 225
- References
- 0
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: On the Spectral Subtraction of Water from the FT-IR Spectra of Aqueous Solutions of Proteins. https://app.cochat.ai/papers/10.1366/0003702894202814
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- Dousseau, F., Therrien, M., & Pézolet, M. (1989). On the Spectral Subtraction of Water from the FT-IR Spectra of Aqueous Solutions of Proteins. Applied Spectroscopy. https://doi.org/10.1366/0003702894202814
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
