An Evaluation of Biofilm Development Utilizing Non-destructive Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy
Abstract
Chemical changes that occur within a microbial biofilm during development on a germanium internal reflection element (IRE) were monitored by attenuated total reflection Fourier transform infrared spec‐troscopy (ATR/FT‐IR) for 188 h. The amount of protein detected at the IRE/medium interface increased throughout the course of the experiment. Extracellular polysaccharides were mainly produced during the initial stages of biofilm development. These results demonstrate that changes in metabolic activity of surface‐associated bacteria during biofilm development on surfaces exposed to a flowing bulk aqueous phase can be evaluated by ATR/FT‐IR.
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The FTIR strong and broad peak at 1000-1200 cm−1 indicates that the analyzed molecule is a carbohydrate.
“A strong and broad peak at 1000–1200 cm−1 indicates that the analyzed molecule is a carbohydrate, since C-O-C and C-O are present [39].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [39]
A medium peak around 1400 cm−1, a strong and broad peak at 1000-1200 cm−1, and the results of TGA analysis showed that the EPS of L. plantarum EIR/IF-1 is an exopolysaccharide with functional carboxylate groups.
“A medium peak around 1400 cm−1, a strong and broad peak at 1000–1200 cm−1, and the results of the TGA analysis showed that the EPS of L. plantarum EIR/IF-1 is an exopolysaccharide with functional carboxylate groups [35,36,39].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [39]
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“plantarum EIR/IF-1 is an exopolysaccharide with functional carboxylate groups [35,36,39].”
Background
- Authors
- Bremer, P. J., & Geesey, G. G.
- Affiliations
- California State University, Long Beach (US)
- ORCID
- Bremer, P J
- Venue
- Biofouling
- Publisher
- Informa UK Limited
- Published
- 1991-04
- Volume
- 3
- Issue
- 2
- Pages
- 89-100
- ISSN
- 0892-7014 (ISSN-L)
- 1029-2454
- OpenAlex
- W2020922532
- Cited by
- 96
- References
- 28
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 3 · 2022: 11 · 2021: 1 · 2020: 6 · 2019: 3 · 2018: 1 · 2017: 5 · 2016: 2 · 2015: 9 · 2014: 5
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biofouling (publishedVersion)
- Montana State University ScholarWorks (Montana State University) (submittedVersion)
- Funders
- National Science Foundation
- International Copper Association
- Division of Materials Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- biofilm growth, non-destructive monitoring, attenuated total reflectance infrared spectroscopy, exopolysaccharides, surface-associated bacteria, bacterial metabolic activity
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