Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 190

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Transcriptome Analysis of Mouse Stem Cells and Early Embryos

Open access
Sharov, Alexei A, Piao, Yulan, Matoba, Ryo, Dudekula, Dawood B, Qian, Yong, VanBuren, Vincent, Falco, Geppino, Martin, Patrick R, Stagg, Carole A, Bassey, Uwem C, Wang, Yuxia, Carter, Mark G, Hamatani, Toshio, Aiba, Kazuhiro, Akutsu, Hidenori, Sharova, Lioudmila, Tanaka, Tetsuya S, Kimber, Wendy L, Yoshikawa, Toshiyuki, Jaradat, Saied A, Pantano, Serafino, Nagaraja, Ramaiah, Boheler, Kenneth R, Taub, Dennis, Hodes, Richard J, Longo, Dan L, Schlessinger, David, Keller, Jonathan, Klotz, Emily, Kelsoe, Garnett, Umezawa, Akihiro, Vescovi, Angelo L, Rossant, Janet, Kunath, Tilo, Hogan, Brigid L. M, Curci, Anna, D'Urso, Michele, Kelso, Janet, Hide, Winston, Ko, Minoru S. H·2003·PLoS Biology
DOI 10.1371/journal.pbio.0000074
Open this paper in CoChat — free account

Abstract

Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public sequences to produce an index of approximately 30,000 total mouse genes that includes 977 previously unidentified genes. Analysis of gene expression levels by EST frequency identifies genes that characterize preimplantation embryos, embryonic stem cells, and adult stem cells, thus providing potential markers as well as clues to the functional features of these cells. Principal component analysis identified a set of 88 genes whose average expression levels decrease from oocytes to blastocysts, stem cells, postimplantation embryos, and finally to newborn tissues. This can be a first step towards a possible definition of a molecular scale of cellular potency. The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells. The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pbio.0000074

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 2 references

Cited by

See all 190

Metadata

Authors
Sharov, A. A., Piao, Y., Matoba, R., Dudekula, D. B., Qian, Y., VanBuren, V., Falco, G., Martin, P. R., Stagg, C. A., Bassey, U. C., Wang, Y., Carter, M. G., Hamatani, T., Aiba, K., Akutsu, H., Sharova, L., Tanaka, T. S., Kimber, W. L., Yoshikawa, T., Jaradat, S. A., Pantano, S., Nagaraja, R., Boheler, K. R., Taub, D., Hodes, R. J., Longo, D. L., Schlessinger, D., Keller, J., Klotz, E., Kelsoe, G., Umezawa, A., Vescovi, A. L., Rossant, J., Kunath, T., Hogan, B. L. M., Curci, A., D'Urso, M., Kelso, J., Hide, W., & Ko, M. S. H.
Venue
PLoS Biology
Publisher
Public Library of Science (PLoS)
Published
2003-12-22
Volume
1
Issue
3
Pages
e74
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
DOI
10.1371/journal.pbio.0000074
OpenAlex
W2094453322
Cited by
190
References
2
FWCI
7.51
Type
journal article
Language
en
Open access
gold

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Transcriptome Analysis of Mouse Stem Cells and Early Embryos. https://app.cochat.ai/papers/10.1371/journal.pbio.0000074
The paper
Sharov, A. A., Piao, Y., Matoba, R., Dudekula, D. B., Qian, Y., VanBuren, V., Falco, G., Martin, P. R., Stagg, C. A., Bassey, U. C., Wang, Y., Carter, M. G., Hamatani, T., Aiba, K., Akutsu, H., Sharova, L., Tanaka, T. S., Kimber, W. L., Yoshikawa, T., . . . Ko, M. S. H. (2003). Transcriptome Analysis of Mouse Stem Cells and Early Embryos. PLoS Biology. https://doi.org/10.1371/journal.pbio.0000074

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.