The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
A Chromosomal Memory Triggered by Xist Regulates Histone Methylation in X Inactivation
Open accessAbstract
We have elucidated the kinetics of histone methylation during X inactivation using an inducible Xist expression system in mouse embryonic stem (ES) cells. Previous reports showed that the ability of Xist to trigger silencing is restricted to an early window in ES cell differentiation. Here we show that this window is also important for establishing methylation patterns on the potential inactive X chromosome. By immunofluorescence and chromatin immunoprecipitation experiments we show that histone H3 lysine 27 trimethylation (H3K27m3) and H4 lysine 20 monomethylation (H4K20m1) are associated with Xist expression in undifferentiated ES cells and mark the initiation of X inactivation. Both marks depend on Xist RNA localisation but are independent of silencing. Induction of Xist expression after the initiation window leads to a markedly reduced ability to induce H3K27m3, whereas expression before the restrictive time point allows efficient H3K27m3 establishment. Our data show that Xist expression early in ES cell differentiation establishes a chromosomal memory, which is maintained in the absence of silencing. One consequence of this memory is the ability to introduce H3K27m3 efficiently after the restrictive time point on the chromosome that has expressed Xist early. Our results suggest that this silencing-independent chromosomal memory has important implications for the maintenance of X inactivation, where previously self-perpetuating heterochromatin structures were viewed as the principal form of memory.
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Cites
Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
Cao, R., Wang, L., Wang, H., Xia, L., Erdjument-Bromage, H., Tempst, P., Jones, R. S., & Zhang, Y. (2002) · Science · doi:10.1126/science.1076997 · Cited by 3,775
Active Genes Are Tri-Methylated at K4 of Histone H3
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Histone Methyltransferase Activity of a Drosophila Polycomb Group Repressor Complex
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Requirement for Xist in X chromosome inactivation
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Role of Histone H3 Lysine 27 Methylation in X Inactivation
Plath, K., Fang, J., Mlynarczyk-Evans, S. K., Cao, R., Worringer, K. A., Wang, H., de la Cruz, C. C., Otte, A. P., Panning, B., & Zhang, Y. (2003) · Science · doi:10.1126/science.1084274 · Cited by 1,195
Partitioning and Plasticity of Repressive Histone Methylation States in Mammalian Chromatin
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Self-citationRequirement for Xist in X chromosome inactivation
(1996) · Trends in Genetics · doi:10.1016/0168-9525(96)81414-4 · Cited by 970
Cited by
The Polycomb complex PRC2 and its mark in life
Margueron, R., & Reinberg, D. (2011) · Nature · doi:10.1038/nature09784 · Cited by 3,347
Stability and flexibility of epigenetic gene regulation in mammalian development
Reik, W. (2007) · Nature · doi:10.1038/nature05918 · Cited by 1,963
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin
Schotta, G., Lachner, M., Sarma, K., Ebert, A., Sengupta, R., Reuter, G., Reinberg, D., & Jenuwein, T. (2004) · Genes & Development · doi:10.1101/gad.300704 · Cited by 1,031
Self-citationde Napoles, M., Mermoud, J. E., Wakao, R., Tang, Y. A., Endoh, M., Appanah, R., Nesterova, T. B., Silva, J., Otte, A. P., Vidal, M., et al. (2004) · Developmental Cell · doi:10.1016/j.devcel.2004.10.005 · Cited by 888
Mutant nuclear lamin A leads to progressive alterations of epigenetic control in premature aging
Shumaker, D. K., Dechat, T., Kohlmaier, A., Adam, S. A., Bozovsky, M. R., Erdos, M. R., Eriksson, M., Goldman, A. E., Khuon, S., Collins, F. S., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602569103 · Cited by 791
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Metadata
- Authors
- Kohlmaier, A., Savarese, F., Lachner, M., Martens, J., Jenuwein, T., & Wutz, A.
- Venue
- PLoS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2004-07-13
- Volume
- 2
- Issue
- 7
- Pages
- e171
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W1986801877
- Cited by
- 386
- References
- 33
- FWCI
- 7.43
- Type
- journal article
- Language
- en
- Open access
- gold
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- Kohlmaier, A., Savarese, F., Lachner, M., Martens, J., Jenuwein, T., & Wutz, A. (2004). A Chromosomal Memory Triggered by Xist Regulates Histone Methylation in X Inactivation. PLoS Biology. https://doi.org/10.1371/journal.pbio.0020171
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