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Cited by 103Circadian rhythm and melatoninZebrafish Biomedical Research ApplicationsDevelopmental Biology and Gene Regulation

Light-dependent development of circadian gene expression in transgenic zebrafish

Open access
Kaneko, Maki, Cahill, Gregory M·2005·PLoS Biology
DOI 10.1371/journal.pbio.0030034PMC PMC546037
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Abstract

The roles of environmental stimuli in initiation and synchronization of circadian oscillation during development appear to vary among different rhythmic processes. In zebrafish, a variety of rhythms emerge in larvae only after exposure to light-dark (LD) cycles, whereas zebrafish period3 (per3) mRNA has been reported to be rhythmic from day 1 of development in constant conditions. We generated transgenic zebrafish in which expression of the firefly luciferase (luc) gene is driven by the zebrafish per3 promoter. Live larvae from these lines are rhythmically bioluminescent, providing the first vertebrate system for high-throughput measurement of circadian gene expression in vivo. Circadian rhythmicity in constant conditions was observed only after 5-6 d of development, and only if the fish were exposed to LD signals after day 4. Regardless of light exposure, a novel developmental profile was observed, with low expression during the first few days and a rapid increase when active swimming begins. Ambient temperature affected the developmental profile and overall levels of per3 and luc mRNA, as well as the critical days in which LD cycles were needed for robust bioluminescence rhythms. In summary, per3-luc zebrafish has revealed complex interactions among developmental events, light, and temperature in the expression of a clock gene.

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  • Bioluminescence has met with only limited success in the zebrafish system.

    “In addition, bioluminescence has met with only limited success in the zebrafish system (Creton et al., 1997; Kaneko and Cahill, 2005”

    From Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis (2008) · cites this paper as Kaneko and Cahill, 2005

    The Discussion (page unavailable) describes the authors’ bioluminescent transgenic zebrafish as a high-throughput system for studying circadian gene expression in whole animals, contrary to the broad claim of only limited success. It notes a narrower limitation: embryos were unsuitable for circadian studies with these lines, whereas older larvae produced robust rhythms. (LLM, conf=0.82)

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Authors
Kaneko, M., & Cahill, G. M.
Affiliations
University of Houston (US)
Corresponding author
Cahill, Gregory M
ORCID
Kaneko, Maki
Venue
PLoS Biology
Publisher
Public Library of Science (PLoS)
Published
2005-02-01
Volume
3
Issue
2
Pages
e34
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
DOI
10.1371/journal.pbio.0030034
PMC
PMC546037
OpenAlex
W2127982832
Cited by
103
References
0
Citations by year
2026: 2 · 2025: 4 · 2024: 7 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 17 · 2019: 7 · 2018: 2 · 2017: 7 · 2016: 2 · 2015: 3 · 2014: 5 · 2013: 5 · 2012: 3
FWCI
2.10
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Life Sciences Research Foundation
  • National Institutes of Health
  • National Institute of Mental Health (R01MH060939, MH60939)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
circadian rhythm, clock gene expression, zebrafish larvae, light-dark cycle, developmental timing, bioluminescence, transgenic zebrafish, ambient temperature, swimming activity, constant darkness
MeSH terms
Animals, Circadian Rhythm, Gene Expression Regulation, Light, Luciferases, Molecular Sequence Data, Nuclear Proteins, Promoter Regions, Genetic, RNA, Messenger, Zebrafish, Genes, Reporter, Zebrafish Proteins, Animals, Genetically Modified, Fireflies

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