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Alignment between PIN1 Polarity and Microtubule Orientation in the Shoot Apical Meristem Reveals a Tight Coupling between Morphogenesis and Auxin Transport

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Heisler, Marcus G., Hamant, Olivier, Krupinski, Pawel, Uyttewaal, Magalie, Ohno, Carolyn, Jönsson, Henrik, Traas, Jan, Meyerowitz, Elliot M.·2010·PLoS Biology
DOI 10.1371/journal.pbio.1000516PMC PMC2957402
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Abstract

Morphogenesis during multicellular development is regulated by intercellular signaling molecules as well as by the mechanical properties of individual cells. In particular, normal patterns of organogenesis in plants require coordination between growth direction and growth magnitude. How this is achieved remains unclear. Here we show that in Arabidopsis thaliana, auxin patterning and cellular growth are linked through a correlated pattern of auxin efflux carrier localization and cortical microtubule orientation. Our experiments reveal that both PIN1 localization and microtubule array orientation are likely to respond to a shared upstream regulator that appears to be biomechanical in nature. Lastly, through mathematical modeling we show that such a biophysical coupling could mediate the feedback loop between auxin and its transport that underlies plant phyllotaxis.

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Authors
Heisler, M. G., Hamant, O., Krupinski, P., Uyttewaal, M., Ohno, C., Jönsson, H., Traas, J., & Meyerowitz, E. M.
Affiliations
  • California Institute of Technology (US)
  • École Normale Supérieure de Lyon (FR)
  • Lund University (SE)
Corresponding author
  • Jönsson, Henrik
  • Traas, Jan
  • Meyerowitz, Elliot M.
ORCID
Venue
PLoS Biology
Publisher
Public Library of Science (PLoS)
Published
2010-10-19
Volume
8
Issue
10
Pages
e1000516
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
DOI
10.1371/journal.pbio.1000516
PMC
PMC2957402
OpenAlex
W1972112316
Cited by
459
References
1
Citations by year
2026: 12 · 2025: 15 · 2024: 24 · 2023: 25 · 2022: 25 · 2021: 44 · 2020: 24 · 2019: 42 · 2018: 29 · 2017: 31 · 2016: 23 · 2015: 22 · 2014: 36 · 2013: 36 · 2012: 40
FWCI
40.81
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • CaltechAUTHORS (California Institute of Technology) (submittedVersion, cc-by)
  • HAL (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
  • Lund University Publications (Lund University) (submittedVersion)
Funders
  • U.S. Department of Energy (FG02-88ER13873)
  • Vetenskapsrådet
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
Keywords
Pin1, auxin efflux carriers, shoot apical meristem, auxin transport, morphogenesis, biomechanics, phyllotaxis, Arabidopsis thaliana, mathematical modeling, cell growth
MeSH terms
Benzamides, Biological Transport, Dinitrobenzenes, Indoleacetic Acids, Microtubules, Models, Theoretical, Morphogenesis, Recombinant Fusion Proteins, Stress, Mechanical, Sulfanilamides, Cell Polarity, Arabidopsis, Meristem, Plant Shoots, Membrane Transport Proteins, Arabidopsis Proteins, Tubulin Modulators

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