The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Identifying off-target effects of etomoxir reveals that carnitine palmitoyltransferase I is essential for cancer cell proliferation independent of β-oxidation
Open accessAbstract
It has been suggested that some cancer cells rely upon fatty acid oxidation (FAO) for energy. Here we show that when FAO was reduced approximately 90% by pharmacological inhibition of carnitine palmitoyltransferase I (CPT1) with low concentrations of etomoxir, the proliferation rate of various cancer cells was unaffected. Efforts to pharmacologically inhibit FAO more than 90% revealed that high concentrations of etomoxir (200 μM) have an off-target effect of inhibiting complex I of the electron transport chain. Surprisingly, however, when FAO was reduced further by genetic knockdown of CPT1, the proliferation rate of these same cells decreased nearly 2-fold and could not be restored by acetate or octanoic acid supplementation. Moreover, CPT1 knockdowns had altered mitochondrial morphology and impaired mitochondrial coupling, whereas cells in which CPT1 had been approximately 90% inhibited by etomoxir did not. Lipidomic profiling of mitochondria isolated from CPT1 knockdowns showed depleted concentrations of complex structural and signaling lipids. Additionally, expression of a catalytically dead CPT1 in CPT1 knockdowns did not restore mitochondrial coupling. Taken together, these results suggest that transport of at least some long-chain fatty acids into the mitochondria by CPT1 may be required for anabolic processes that support healthy mitochondrial function and cancer cell proliferation independent of FAO.
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Cites
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Fatty acid oxidation: An emerging facet of metabolic transformation in cancer
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Metadata
- Authors
- Yao, C.-H., Liu, G.-Y., Wang, R., Moon, S. H., Gross, R. W., & Patti, G. J.
- Venue
- PLOS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2018-03-29
- Volume
- 16
- Issue
- 3
- Pages
- e2003782
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W2794453923
- Cited by
- 206
- References
- 9
- FWCI
- 6.43
- Type
- journal article
- Language
- en
- Open access
- gold
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- Yao, C.-H., Liu, G.-Y., Wang, R., Moon, S. H., Gross, R. W., & Patti, G. J. (2018). Identifying off-target effects of etomoxir reveals that carnitine palmitoyltransferase I is essential for cancer cell proliferation independent of β-oxidation. PLOS Biology. https://doi.org/10.1371/journal.pbio.2003782
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