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Cited by 37Biotin and Related StudiesMicrotubule and mitosis dynamicsGenetic and Kidney Cyst Diseases

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A modified TurboID approach identifies tissue-specific centriolar components in C. elegans

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Holzer, Elisabeth, Rumpf-Kienzl, Cornelia, Falk, Sebastian, Dammermann, Alexander·2022·PLOS Genetics
DOI 10.1371/journal.pgen.1010150PMC PMC9020716

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Abstract

Proximity-dependent labeling approaches such as BioID have been a great boon to studies of protein-protein interactions in the context of cytoskeletal structures such as centrosomes which are poorly amenable to traditional biochemical approaches like immunoprecipitation and tandem affinity purification. Yet, these methods have so far not been applied extensively to invertebrate experimental models such as C . elegans given the long labeling times required for the original promiscuous biotin ligase variant BirA*. Here, we show that the recently developed variant TurboID successfully probes the interactomes of both stably associated (SPD-5) and dynamically localized (PLK-1) centrosomal components. We further develop an indirect proximity labeling method employing a GFP nanobody-TurboID fusion, which allows the identification of protein interactors in a tissue-specific manner in the context of the whole animal. Critically, this approach utilizes available endogenous GFP fusions, avoiding the need to generate multiple additional strains for each target protein and the potential complications associated with overexpressing the protein from transgenes. Using this method, we identify homologs of two highly conserved centriolar components, Cep97 and BLD10/Cep135, which are present in various somatic tissues of the worm. Surprisingly, neither protein is expressed in early embryos, likely explaining why these proteins have escaped attention until now. Our work expands the experimental repertoire for C . elegans and opens the door for further studies of tissue-specific variation in centrosome architecture.

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Metadata

Authors
Holzer, E., Rumpf-Kienzl, C., Falk, S., & Dammermann, A.
Affiliations
University of Vienna (AT)
Corresponding author
Dammermann, Alexander
ORCID
Venue
PLOS Genetics
Publisher
Public Library of Science (PLoS)
Published
2022-04-20
Volume
18
Issue
4
Pages
e1010150
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.1010150
PMC
PMC9020716
OpenAlex
W4226102213
Cited by
37
References
3
Citations by year
2026: 8 · 2025: 12 · 2024: 8 · 2023: 6 · 2022: 3
FWCI
3.87
Type
journal article
Language
en
Update notices
correction (2023-02-13)
Open access
Licence
Locations
  • PLoS Genetics (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Austrian Science Fund (P30760-B28, P34526-B)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
TurboID, centrosome, centrioles, protein-protein interaction, Caenorhabditis elegans, PLK1, embryonic gene expression
MeSH terms
Polo-Like Kinase 1, Animals, Centrioles, Caenorhabditis elegans, Protein Serine-Threonine Kinases, Centrosome, Biotinylation, Caenorhabditis elegans Proteins

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