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Cited by 0Developmental Biology and Gene RegulationReproductive Biology and FertilityGenetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

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MYCBP interacts with Sakura and Otu and is essential for germline stem cell renewal and differentiation and oogenesis

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Azlan, Azali, Fukunaga, Ryuya·2025·PLOS Genetics
DOI 10.1371/journal.pgen.1011792PMC PMC12236646

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Abstract

The self-renewal and differentiation of germline stem cells (GSCs) are tightly regulated during oogenesis. The Drosophila female germline provides a powerful model to study these regulatory mechanisms. We previously identified Sakura (also known as Bourbon/CG14545) as a crucial factor for maintenance and differentiation of GSCs and oogenesis, and demonstrated that Sakura binds to Ovarian Tumor (Otu), another essential regulator of these processes. Here, we identify MYCBP (c-Myc binding protein) as an additional essential component of this regulatory network. We show that MYCBP physically associates with itself, Sakura, and Otu, forming binary and ternary complexes including a MYCBP•Sakura•Otu complex. MYCBP is highly expressed in the ovary, and mycbp null mutant females exhibit rudimentary ovaries with germline-less and tumorous ovarioles, fail to produce eggs, and are completely sterile. Germline-specific depletion of mycbp disrupts Dpp/BMP signaling, causing aberrant expression of bag-of-marbles ( bam ) and leading to defective differentiation and GSC loss. In addition, mycbp is required for female-specific splicing of sex-lethal ( sxl ), a master regulator of sex identity determination. These phenotypes closely resemble those observed in sakura and otu mutants. Together, our findings reveal that MYCBP functions in concert with Sakura and Otu to coordinate self-renewal and differentiation of GSCs and oogenesis in Drosophila .

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Figures

Fig 1. MYCBP expression pattern and mutant allele (A) Schematic illustration of Drosophila ovary and germarium. Each female has a pair of ovaries, each consisting of 12–16 ovarioles (cyan). The germarium (outlined in magenta) is located at the anterior tip of each ovariole and contains both germ cells and somatic cells. Germ cells include germline stem cells (GSCs), cystoblasts, cysts, and differentiating oocytes. Somatic cells include terminal filament (TF) cells, cap cells (CCc), escort cells (ECs), and follicle cells (FCs). GSCs and cystoblasts have spherical, unbranched spectrosomes, whereas cysts posesss branched fusomes. The distinct regions of the germarium—1, 2a, 2b, and 3—are indicated. (B) Drosophila MYCBP (CG17202) protein and the null mutant allele generated in this study. (C) Western blot of dissected fly tissues. (D) Confocal images of germaria from mycbp-EGFP transgenic flies. MYCBP-EGFP (green), Hts (red), and DAPI (blue). Scale bar: 10 μm. (E) Confocal images of egg chambers from mycbp-EGFP transgenic flies. MYCBP-EGFP (green) and DAPI (blue). MYCBP-EGFP is highly expressed in nurse cells, enriched in the developing oocyte (yellow arrow), and is also detectable in somatic follicle cells. Scale bar: 20 μm.
Fig 1. MYCBP expression pattern and mutant allele (A) Schematic illustration of Drosophila ovary and germarium. Each female has a pair of ovaries, each consisting of 12–16 ovarioles (cyan). The germarium (outlined in magenta) is located at the anterior tip of each ovariole and contains both germ cells and somatic cells. Germ cells include germline stem cells (GSCs), cystoblasts, cysts, and differentiating oocytes. Somatic cells include terminal filament (TF) cells, cap cells (CCc), escort cells (ECs), and follicle cells (FCs). GSCs and cystoblasts have spherical, unbranched spectrosomes, whereas cysts posesss branched fusomes. The distinct regions of the germarium—1, 2a, 2b, and 3—are indicated. (B) Drosophila MYCBP (CG17202) protein and the null mutant allele generated in this study. (C) Western blot of dissected fly tissues. (D) Confocal images of germaria from mycbp-EGFP transgenic flies. MYCBP-EGFP (green), Hts (red), and DAPI (blue). Scale bar: 10 μm. (E) Confocal images of egg chambers from mycbp-EGFP transgenic flies. MYCBP-EGFP (green) and DAPI (blue). MYCBP-EGFP is highly expressed in nurse cells, enriched in the developing oocyte (yellow arrow), and is also detectable in somatic follicle cells. Scale bar: 20 μm.CC BY · PubMed Central
Fig 2. MYCBP interacts with Otu and Sakura (A) Co-immunoprecipitation (co-IP) using anti-GFP magnetic beads followed by Western blotting. Ovary lysates expressing MYCBP-EGFP in mycbp+/+ background and those from w1118 negative control were analyzed. (B) Co-IP using anti-MYCBP antibodies and ovary lysates from w1118. IgG was used as a negative control IP. (C-E) Structures of (C) MYCBP•Otu(N), (D) MYCBP•MYCBP•Otu(N), and (E) MYCBP•Sakura predicted using Alphafold. (F, G) Schematic diagrams of full-length and fragment constructs of (F) Otu and (G) Sakura used in co-IP assays. The binding assay results from (H) is summarized. (G) Full-length Sakura and Sakura fragments tested in co-immunoprecipitation assays. N: N-terminal, M: middle, C: C-terminal. The co-IP assay results from (H) and (I) are summarized. (H) Co-IP using anti-HA beads followed by Western blotting. S2 cell lysates co-expressing MYCBP-mCherry-3xHA and 3xFLAG-Otu (full-length or fragments) were tested. 3XFLAG-EGFPserved as a negative control. (I) Co-IP using anti-FLAG beads followed by Western blotting. S2 cell lysates co-expressing 3xFLAG-EGFP-MYCBP and Sakura-mCherry-3xHA (full-length or fragments) were tested. mCherry-3xHA served as a negative control.
Fig 2. MYCBP interacts with Otu and Sakura (A) Co-immunoprecipitation (co-IP) using anti-GFP magnetic beads followed by Western blotting. Ovary lysates expressing MYCBP-EGFP in mycbp+/+ background and those from w1118 negative control were analyzed. (B) Co-IP using anti-MYCBP antibodies and ovary lysates from w1118. IgG was used as a negative control IP. (C-E) Structures of (C) MYCBP•Otu(N), (D) MYCBP•MYCBP•Otu(N), and (E) MYCBP•Sakura predicted using Alphafold. (F, G) Schematic diagrams of full-length and fragment constructs of (F) Otu and (G) Sakura used in co-IP assays. The binding assay results from (H) is summarized. (G) Full-length Sakura and Sakura fragments tested in co-immunoprecipitation assays. N: N-terminal, M: middle, C: C-terminal. The co-IP assay results from (H) and (I) are summarized. (H) Co-IP using anti-HA beads followed by Western blotting. S2 cell lysates co-expressing MYCBP-mCherry-3xHA and 3xFLAG-Otu (full-length or fragments) were tested. 3XFLAG-EGFPserved as a negative control. (I) Co-IP using anti-FLAG beads followed by Western blotting. S2 cell lysates co-expressing 3xFLAG-EGFP-MYCBP and Sakura-mCherry-3xHA (full-length or fragments) were tested. mCherry-3xHA served as a negative control.CC BY · PubMed Central
Fig 3. Sequential co-immunoprecipitation shows MYCBP, Sakura, and Otu form a ternary complex (A) MYCBP•Sakura•Otu structure predicted by AlphaFold. (B) Sequential co-IP followed by Western blotting. S2 cell lysates co-expressing Sakura-EGFP-HRV3Csite-3xFLAG, MYCBP-mCherry-3xHA, and Myc-Otu were used. EGFP-HRV3csite-3xFLAG served as a negative control. The first IP was performed using anti-FLAG beads and eluted using HRV3C protease. Second IP was performed with anti-HA beads. Non-specific bands are marked with *.
Fig 3. Sequential co-immunoprecipitation shows MYCBP, Sakura, and Otu form a ternary complex (A) MYCBP•Sakura•Otu structure predicted by AlphaFold. (B) Sequential co-IP followed by Western blotting. S2 cell lysates co-expressing Sakura-EGFP-HRV3Csite-3xFLAG, MYCBP-mCherry-3xHA, and Myc-Otu were used. EGFP-HRV3csite-3xFLAG served as a negative control. The first IP was performed using anti-FLAG beads and eluted using HRV3C protease. Second IP was performed with anti-HA beads. Non-specific bands are marked with *.CC BY · PubMed Central
Fig 4. mycbpnull female flies are sterile and have rudimentary ovaries. (A) Western blot of ovary lysates. (B, C) Female fertility assays. (B) The number of eggs laid by test females mated with wild-type (OregonR) males. (C) Hatching rate of the eggs. Mean ± SD (n = 3). P-value < 0.001 (Student’s t-test, unpaired, two-tailed) is indicated by ***. (D) Stereomicroscope images of dissected ovaries. Scale bar: 200 μm.
Fig 4. mycbpnull female flies are sterile and have rudimentary ovaries. (A) Western blot of ovary lysates. (B, C) Female fertility assays. (B) The number of eggs laid by test females mated with wild-type (OregonR) males. (C) Hatching rate of the eggs. Mean ± SD (n = 3). P-value < 0.001 (Student’s t-test, unpaired, two-tailed) is indicated by ***. (D) Stereomicroscope images of dissected ovaries. Scale bar: 200 μm.CC BY · PubMed Central
Fig 5. mycbpnull ovaries are germless and tumorous (A, B) Confocal images of ovaries from control (mycbpnull/+) and mycbpnull/null flies expressing Vasa-EGFP. Vasa-EGFP (green) and DAPI (blue). Yellow, orange, and. cyan stars mark normal ovarioles, tumorous, and germless ovarioles, respectively, in Figure 5. (B) Higher-magnification images of germaria. Scale bars: 50 μm (A), 10 μm (B). (C) Confocal images of ovaries from control (mycbpnull/+) and mycbpnull/null flies stained with anti-Hts to label spectrosomes and fusomes. Hts (red) and DAPI (blue). Germaria are outlined. Scale bars: 20 μm. (D) Percentage of normal, germless, and tumorous ovarioles in indicated genotypes (ages 2–5 days). (E) Quantification of GSC-like cells per germarium (ages 2–5 days). (F) Confocal images of ovaries stained with anti-cleaved Caspase-3. Cleaved caspase-3 (red) and DAPI (blue). Scale bars: 50 μm.
Fig 5. mycbpnull ovaries are germless and tumorous (A, B) Confocal images of ovaries from control (mycbpnull/+) and mycbpnull/null flies expressing Vasa-EGFP. Vasa-EGFP (green) and DAPI (blue). Yellow, orange, and. cyan stars mark normal ovarioles, tumorous, and germless ovarioles, respectively, in Figure 5. (B) Higher-magnification images of germaria. Scale bars: 50 μm (A), 10 μm (B). (C) Confocal images of ovaries from control (mycbpnull/+) and mycbpnull/null flies stained with anti-Hts to label spectrosomes and fusomes. Hts (red) and DAPI (blue). Germaria are outlined. Scale bars: 20 μm. (D) Percentage of normal, germless, and tumorous ovarioles in indicated genotypes (ages 2–5 days). (E) Quantification of GSC-like cells per germarium (ages 2–5 days). (F) Confocal images of ovaries stained with anti-cleaved Caspase-3. Cleaved caspase-3 (red) and DAPI (blue). Scale bars: 50 μm.CC BY · PubMed Central
Fig 6. Loss of mycbp disrupts piRNA-mediated transposon silencing and sxl splicing (A) Schematic of the Burdock sensor construct and representative images of sensor expression in ovaries from control (yRNAi) and mycbpRNAi flies. The Burdock sensor harbors a nanos promoter, a nuclear localization signal (NLS) appended to GFP and β-gal coding sequences, and a target sequence for Burdock piRNAs in the 3’UTR. RNAi was driven in the germline using UAS-Dcr2, NGT-Gal4, and nos-Gal4-VP16. GFP (green), β-gal (red), and DAPI (blue). Scale bars: 20 μm. 0/3 control samples showed sensor activation; 15/15 mycbpRNAi samples did. (B) RT-PCR analysis of sxl alternative splicing in ovaries and testes.
Fig 6. Loss of mycbp disrupts piRNA-mediated transposon silencing and sxl splicing (A) Schematic of the Burdock sensor construct and representative images of sensor expression in ovaries from control (yRNAi) and mycbpRNAi flies. The Burdock sensor harbors a nanos promoter, a nuclear localization signal (NLS) appended to GFP and β-gal coding sequences, and a target sequence for Burdock piRNAs in the 3’UTR. RNAi was driven in the germline using UAS-Dcr2, NGT-Gal4, and nos-Gal4-VP16. GFP (green), β-gal (red), and DAPI (blue). Scale bars: 20 μm. 0/3 control samples showed sensor activation; 15/15 mycbpRNAi samples did. (B) RT-PCR analysis of sxl alternative splicing in ovaries and testes.CC BY · PubMed Central
Fig 7. Germline clonal analysis of mycbpnull (A) Percentage of germaria with marked (GFP-negative) GSC clones at 4, 7, and 14 days after clone induction. P-value < 0.001 (Chi-squared test) is indicated by ***. (B) Confocal images of control and mycbpnull clones. GFP (green), Hts (red), and DAPI (blue). Scale bar: 10 μm. (C) Number of marked (GFP-negative) GSC-like cells per germarium containing marked GSCs at 4, 7, and 14 days after clone induction. GSC-like cells containing round spectrosome were identified by anti-Hts staining. P-value < 0.05, <0.01, and <0.001 indicated by *, **, and *** (Student’s t-test, unpaired, two-tailed).
Fig 7. Germline clonal analysis of mycbpnull (A) Percentage of germaria with marked (GFP-negative) GSC clones at 4, 7, and 14 days after clone induction. P-value < 0.001 (Chi-squared test) is indicated by ***. (B) Confocal images of control and mycbpnull clones. GFP (green), Hts (red), and DAPI (blue). Scale bar: 10 μm. (C) Number of marked (GFP-negative) GSC-like cells per germarium containing marked GSCs at 4, 7, and 14 days after clone induction. GSC-like cells containing round spectrosome were identified by anti-Hts staining. P-value < 0.05, <0.01, and <0.001 indicated by *, **, and *** (Student’s t-test, unpaired, two-tailed).CC BY · PubMed Central
Fig 8. Loss of sakura inhibits Dpp/BMP signaling (A) Schematic of Dpp-mediated bam repression via pMad activation. Cap cells secrete diffusible Decapentaplegic (Dpp), which is received by its receptor, a heterodimer of Thick vein (Tkv) and Punt (Put), in GSCs. The activated Dpp signaling eventually phosphorylates Mother-against-dpp (Mad). The phosphorylated Mad (pMad) represses the transcription of bam. The repression of bam in GSCs is crucial for maintaining their stemness. Cystoblasts do not receive Dpp, and Bam expression is crucial for promoting cystoblast differentiation from GSCs. (B) Confocal images of bam-GFP reporter expression in control (yRNAi) and mycbpRNAi ovaries (Azlan et al. 2024). RNAi was driven in the female germline using UAS-Dcr2 and NGT-Gal4. Bam-GFP (green), Hts (red), and DAPI (blue). Germaria are outlined. Scale bar: 10 μm. (C, D) Confocal images of germaria with control and mycbpnull germline clones stained with (C) anti-Bam and (D) anti-pMad. GFP (green), Bam/pMad (red), and DAPI (blue). Scale bar: 10 μm. (E) Quantification of pMad intensity in GSC clones. Mean ± SD (n = 9 and 13 for FRT82B and FRT82B, mycbpnull, respectively). P-value < 0.001 (Student’s t-test, unpaired, two-tailed) is indicated by ***. (F) Confocal images of germaria with control and mycbpnull germline clones stained with anti-CycA. GFP (green), CycA (red), and DAPI (blue). Scale bar: 10 μm. (G) Quantification of CycA intensity in the germline clones. Mean ± SD (n = 9 and 11 for FRT82B and FRT82B, mycbpnull, respectively). P-value < 0.01 (Student’s t-test, unpaired, two-tailed) is indicated by **. Clones in C, D, and F are GFP-negative.
Fig 8. Loss of sakura inhibits Dpp/BMP signaling (A) Schematic of Dpp-mediated bam repression via pMad activation. Cap cells secrete diffusible Decapentaplegic (Dpp), which is received by its receptor, a heterodimer of Thick vein (Tkv) and Punt (Put), in GSCs. The activated Dpp signaling eventually phosphorylates Mother-against-dpp (Mad). The phosphorylated Mad (pMad) represses the transcription of bam. The repression of bam in GSCs is crucial for maintaining their stemness. Cystoblasts do not receive Dpp, and Bam expression is crucial for promoting cystoblast differentiation from GSCs. (B) Confocal images of bam-GFP reporter expression in control (yRNAi) and mycbpRNAi ovaries (Azlan et al. 2024). RNAi was driven in the female germline using UAS-Dcr2 and NGT-Gal4. Bam-GFP (green), Hts (red), and DAPI (blue). Germaria are outlined. Scale bar: 10 μm. (C, D) Confocal images of germaria with control and mycbpnull germline clones stained with (C) anti-Bam and (D) anti-pMad. GFP (green), Bam/pMad (red), and DAPI (blue). Scale bar: 10 μm. (E) Quantification of pMad intensity in GSC clones. Mean ± SD (n = 9 and 13 for FRT82B and FRT82B, mycbpnull, respectively). P-value < 0.001 (Student’s t-test, unpaired, two-tailed) is indicated by ***. (F) Confocal images of germaria with control and mycbpnull germline clones stained with anti-CycA. GFP (green), CycA (red), and DAPI (blue). Scale bar: 10 μm. (G) Quantification of CycA intensity in the germline clones. Mean ± SD (n = 9 and 11 for FRT82B and FRT82B, mycbpnull, respectively). P-value < 0.01 (Student’s t-test, unpaired, two-tailed) is indicated by **. Clones in C, D, and F are GFP-negative.CC BY · PubMed Central
Fig 9. mycbp is important for late oogenesis (A) Stereomicroscope images of dissected ovaries. Scale bar: 500 μm. yRNAi served as a control. (B, C) Number of stage 14 oocytes per fly (B) and eggs laid (C) in mycbp RNAi knockdown usingby UAS-Dcr2 and TOsk-Gal4. Mean ± SD (n = 5). P-value < 0.001 (Student’s t-test, unpaired, two-tailed) is indicated by ***. (D) Confocal images of ovaries stained with phalloidin (F-Actin, red), anti-Orb (Green), and DAPI (blue). Yellow arrows indicate normal Orb localization; white arrowheads indicate cytoskeletal disorganization and loss of Orb localization. Scale bar: 100 μm.
Fig 9. mycbp is important for late oogenesis (A) Stereomicroscope images of dissected ovaries. Scale bar: 500 μm. yRNAi served as a control. (B, C) Number of stage 14 oocytes per fly (B) and eggs laid (C) in mycbp RNAi knockdown usingby UAS-Dcr2 and TOsk-Gal4. Mean ± SD (n = 5). P-value < 0.001 (Student’s t-test, unpaired, two-tailed) is indicated by ***. (D) Confocal images of ovaries stained with phalloidin (F-Actin, red), anti-Orb (Green), and DAPI (blue). Yellow arrows indicate normal Orb localization; white arrowheads indicate cytoskeletal disorganization and loss of Orb localization. Scale bar: 100 μm.CC BY · PubMed Central
Fig 10. MYCBP is required for Sakura level Western blot of ovary lysates. The sakura RNAi #1 (VDRC: v39727) and #2 (VDRC: v103660). Non-specific bands are marked (*). Sakura and alpha-Tubulin images in (E), Sakura, Otu, and alpha-Tubulin images in (F), Otu and alpha-Tubulin images in (H), Sakura, Otu, and alpha-Tubulin images in (I), are from (Azlan et al. 2024).
Fig 10. MYCBP is required for Sakura level Western blot of ovary lysates. The sakura RNAi #1 (VDRC: v39727) and #2 (VDRC: v103660). Non-specific bands are marked (*). Sakura and alpha-Tubulin images in (E), Sakura, Otu, and alpha-Tubulin images in (F), Otu and alpha-Tubulin images in (H), Sakura, Otu, and alpha-Tubulin images in (I), are from (Azlan et al. 2024).CC BY · PubMed Central
Fig 11. Mutual dependance of MYCBP and Sakura level and localization (A) Confocal images of egg chambers with control and mycbpnull germline clones expressing Sakura-EGFP. Marked clones (RFP-negative) are outlined. RFP (red), Sakura-EGFP (green), and DAPI (blue). Scale bar: 20 μm. Yellow arrow: normal Sakura-EGFP posterior enrichment; magenta arrow: reduced Sakura-EGFP signal. Fly genotypes used: hs-flp/w; sakura-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; sakura-EGFP/+; FRT82B, ubi-RFP/FRT82B, mycbpnull. (B) Confocal images of egg chambers with control and sakuranull germline clones expressing MYCBP-EGFP. Marked clones (RFP-negative) are outlined. RFP (red), MYCBP-EGFP (green), and DAPI (blue). Scale bar: 20 μm. Yellow arrow: normal MYCBP-EGFP posterior enrichment; magenta arrow: reduced levels and loss of posterior localization of MYCBP-EGFP. Fly genotypes used: hs-flp/w; mycbp-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; mycbp-EGFP/+; FRT82B, ubi-RFP/FRT82B, sakuranull.
Fig 11. Mutual dependance of MYCBP and Sakura level and localization (A) Confocal images of egg chambers with control and mycbpnull germline clones expressing Sakura-EGFP. Marked clones (RFP-negative) are outlined. RFP (red), Sakura-EGFP (green), and DAPI (blue). Scale bar: 20 μm. Yellow arrow: normal Sakura-EGFP posterior enrichment; magenta arrow: reduced Sakura-EGFP signal. Fly genotypes used: hs-flp/w; sakura-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; sakura-EGFP/+; FRT82B, ubi-RFP/FRT82B, mycbpnull. (B) Confocal images of egg chambers with control and sakuranull germline clones expressing MYCBP-EGFP. Marked clones (RFP-negative) are outlined. RFP (red), MYCBP-EGFP (green), and DAPI (blue). Scale bar: 20 μm. Yellow arrow: normal MYCBP-EGFP posterior enrichment; magenta arrow: reduced levels and loss of posterior localization of MYCBP-EGFP. Fly genotypes used: hs-flp/w; mycbp-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; mycbp-EGFP/+; FRT82B, ubi-RFP/FRT82B, sakuranull.CC BY · PubMed Central
Fig 12. Otu localization to the developing oocyte depends on MYCBP and Sakura Confocal images of egg chambers with control, mycbpnull, and sakuranull germline clones expressing (A) Otu-EGFP or (B) Otu(ΔTudor)-EGFP. Marked clones (RFP-negative) are outlined. RFP (red), Otu-EGFP or Otu(ΔTudor)-EGFP (green), Orb (white), and DAPI (blue). Scale bar: 20 μm. Yellow arrows: normal posterior enrichment of Otu-EGFP and Otu(ΔTudor)-EGFP; magenta arrows: loss of localization. Orb localization remains intact in mutant clones. Fly genotypes used: hs-flp/w; otu-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; otu-EGFP/+; FRT82B, ubi-RFP/FRT82B, mycbpnull. hs-flp/w; otu-EGFP/+; FRT82B, ubi-RFP/FRT82B, sakuranull. hs-flp/w; otu(ΔTudor)-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; otu(ΔTudor)-EGFP /+; FRT82B, ubi-RFP/FRT82B, mycbpnull. hs-flp/w; otu(ΔTudor)-EGFP /+; FRT82B, ubi-RFP/FRT82B, sakuranull.
Fig 12. Otu localization to the developing oocyte depends on MYCBP and Sakura Confocal images of egg chambers with control, mycbpnull, and sakuranull germline clones expressing (A) Otu-EGFP or (B) Otu(ΔTudor)-EGFP. Marked clones (RFP-negative) are outlined. RFP (red), Otu-EGFP or Otu(ΔTudor)-EGFP (green), Orb (white), and DAPI (blue). Scale bar: 20 μm. Yellow arrows: normal posterior enrichment of Otu-EGFP and Otu(ΔTudor)-EGFP; magenta arrows: loss of localization. Orb localization remains intact in mutant clones. Fly genotypes used: hs-flp/w; otu-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; otu-EGFP/+; FRT82B, ubi-RFP/FRT82B, mycbpnull. hs-flp/w; otu-EGFP/+; FRT82B, ubi-RFP/FRT82B, sakuranull. hs-flp/w; otu(ΔTudor)-EGFP/+; FRT82B, ubi-RFP/FRT82B. hs-flp/w; otu(ΔTudor)-EGFP /+; FRT82B, ubi-RFP/FRT82B, mycbpnull. hs-flp/w; otu(ΔTudor)-EGFP /+; FRT82B, ubi-RFP/FRT82B, sakuranull.CC BY · PubMed Central

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Research works printed but not linked: 1

  1. PA Smith, 1966, Studies on Fused a Mutant Gene Producing Ovarian Tumors in Drosophila Melanogaster, Genetics, 54, 363

Metadata

Authors
Azlan, A., & Fukunaga, R.
Affiliations
Johns Hopkins University (US)
Corresponding author
Fukunaga, Ryuya
ORCID
Venue
PLOS Genetics
Publisher
Public Library of Science (PLoS)
Published
2025-12-01
Volume
21
Issue
12
Pages
e1011792
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.1011792
PMC
PMC12236646
OpenAlex
W4416873099
Cited by
0
References
74
FWCI
0.00
Type
journal article
Language
en
Update notices
new_version (2025-12-04)
Open access
Licence
Locations
  • PLoS Genetics (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institute of General Medical Sciences (R35GM145352)
  • Division of Intramural Research, National Institute of Allergy and Infectious Diseases (R03AI178064)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Keywords
cherry blossom, ovarian neoplasms, germline stem cells, oogenesis, germline stem cell differentiation, bag of marbles, sex determination, protein complex formation, Drosophila ovary, sterility

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