The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
Open accessAbstract
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed protein. We have devised a simple and rapid subcloning strategy to transfer any DNA fragment of interest from an entry clone into an expression vector, without this shortcoming. The strategy is based on the use of type IIs restriction enzymes, which cut outside of their recognition sequence. With proper design of the cleavage sites, two fragments cut by type IIs restriction enzymes can be ligated into a product lacking the original restriction site. Based on this property, a cloning strategy called 'Golden Gate' cloning was devised that allows to obtain in one tube and one step close to one hundred percent correct recombinant plasmids after just a 5 minute restriction-ligation. This method is therefore as efficient as currently used recombination-based cloning technologies but yields recombinant plasmids that do not contain unwanted sequences in the final construct, thus providing precision for this fundamental process of genetic manipulation.
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Cites
Giritch, A., Marillonnet, S., Engler, C., van Eldik, G., Botterman, J., Klimyuk, V., & Gleba, Y. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606631103 · Cited by 419
Self-citationClass-IIS restriction enzymes — a review
Szybalski, W., Kim, S. C., Hasan, N. M., & Podhajska, A. J. (1991) · Gene · doi:10.1016/0378-1119(91)90345-c · Cited by 249
Many Paths to Many Clones: A Comparative Look at High-Throughput Cloning MethodsPreprint
Marsischky, G., & LaBaer, J. (2004) · Genome Research · doi:10.1101/gr.2528804 · Cited by 136
Seamless cloning and gene fusion
Lu, Q. (2005) · Trends in biotechnology · doi:10.1016/j.tibtech.2005.02.008 · Cited by 122
Stemmer, W. P. C., & Morris, S. K. (1992) · PubMed · Cited by 105
Creating seamless junctions independent of restriction sites in PCR cloning
Padgett, K. A., & Sorge, J. A. (1996) · Gene · doi:10.1016/0378-1119(95)00731-8 · Cited by 86
Colwill, K., Wells, C. D., Elder, K., Goudreault, M., Hersi, K., Kulkarni, S., Hardy, W. R., Pawson, T. J., & Morin, G. B. (2006) · BMC Biotechnology · doi:10.1186/1472-6750-6-13 · Cited by 46
Method of artificial DNA spling by directed ligation(SDL)
Lebedenko, E. N., Birikh, K. R., Plutalov, O. V., & Berlin, Y. A. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.24.6757 · Cited by 34
Fromme, T., & Klingenspor, M. (2007) · Journal of Biological Engineering · doi:10.1186/1754-1611-1-7 · Cited by 11
DNA Splicing by Directed Ligation (SDL)
Berlin, Y. A. (1999) · Current Issues in Molecular Biology · doi:10.21775/cimb.001.021 · Cited by 6
Cited by
Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.04.007 · Cited by 2,369
A CRISPR/Cas9 toolkit for multiplex genome editing in plants
Xing, H.-L., Dong, L., Wang, Z.-P., Zhang, H.-Y., Han, C.-Y., Liu, B., Wang, X.-C., & Chen, Q.-J. (2014) · BMC Plant Biology · doi:10.1186/s12870-014-0327-y · Cited by 1,684
A Modular Cloning System for Standardized Assembly of Multigene Constructs
Weber, E., Engler, C., Gruetzner, R., Werner, S., & Marillonnet, S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016765 · Cited by 1,377
Self-citationTAL effectors: customizable proteins for DNA targeting
Bogdanove, A. J., & Voytas, D. F. (2011) · Science · doi:10.1126/science.1204094 · Cited by 984
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015) · ACS Synthetic Biology · doi:10.1021/sb500366v · Cited by 916
Metadata
- Authors
- Engler, C., Kandzia, R., & Marillonnet, S.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2008-11-05
- Volume
- 3
- Issue
- 11
- Pages
- e3647
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2016667239
- Cited by
- 2,389
- References
- 10
- FWCI
- 4.09
- Type
- journal article
- Language
- en
- Open access
- gold
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
