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Macroautophagy-Mediated Degradation of Whole Nuclei in the Filamentous Fungus Aspergillus oryzae

Shoji, Jun-ya, Kikuma, Takashi, Arioka, Manabu, Kitamoto, Katsuhiko·2010·PLoS ONE
DOI 10.1371/journal.pone.0015650PMC PMC3004950
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Figure 1 Autophagy-dependent uptake of EGFP labeling peroxisomes, mitochondria, and nuclei in basal cells of A. oryzae. Subcellular distribution of EGFP labeling peroxisomes (Ai, iv, vii, Bi, iv), mitochondria (Aii, v, viii, Bii, v), and nuclei (Aiii, vi, ix, Biii, vi) in either wild-type (A) or an Aoatg8-deleted background (B). Cultures were incubated at 30°C for either 24 hrs (Ai–vi, Bi–iii) or 48 hrs (Avii–ix, Biv–vi). Bars represent 5 µm.
Figure 1 Autophagy-dependent uptake of EGFP labeling peroxisomes, mitochondria, and nuclei in basal cells of A. oryzae. Subcellular distribution of EGFP labeling peroxisomes (Ai, iv, vii, Bi, iv), mitochondria (Aii, v, viii, Bii, v), and nuclei (Aiii, vi, ix, Biii, vi) in either wild-type (A) or an Aoatg8-deleted background (B). Cultures were incubated at 30°C for either 24 hrs (Ai–vi, Bi–iii) or 48 hrs (Avii–ix, Biv–vi). Bars represent 5 µm.CC BY · PubMed Central
Figure 2 Macroautophagy-mediated uptake of nuclei by vacuoles. A series of superimposed images of EGFP-AoAtg8 and H2B-mDsRed is shown. (A) A crescent of an autophagosomal precursor (green, arrow) matured into a ring-like autophagosome to encircle an apparently intact nucleus (red). Arrowheads point to vacuoles. (B) A ring-like autophagosome (green, arrow) surrounding an apparently intact nucleus (red) disappeared (7 min), followed by dispersal of H2B-fused mDsRed throughout a vacuole. Arrowheads point to vacuoles. Bars represent 5 µm.
Figure 2 Macroautophagy-mediated uptake of nuclei by vacuoles. A series of superimposed images of EGFP-AoAtg8 and H2B-mDsRed is shown. (A) A crescent of an autophagosomal precursor (green, arrow) matured into a ring-like autophagosome to encircle an apparently intact nucleus (red). Arrowheads point to vacuoles. (B) A ring-like autophagosome (green, arrow) surrounding an apparently intact nucleus (red) disappeared (7 min), followed by dispersal of H2B-fused mDsRed throughout a vacuole. Arrowheads point to vacuoles. Bars represent 5 µm.CC BY · PubMed Central
Figure 3 Autophagy supporting the mycelial growth. Wild-type and ΔAoatg8 strains were inoculated and grown on either the CD medium with 0.15% methionine (nutrient-supplied) or its 104 times dilution (nutrient-depleted) for seven days. The colony diameters of each strain at the seventh day are shown. Relative diameters of each strain to the nutrient-supplied medium are shown as percentages. Error bars represent S. D. n = 3.
Figure 3 Autophagy supporting the mycelial growth. Wild-type and ΔAoatg8 strains were inoculated and grown on either the CD medium with 0.15% methionine (nutrient-supplied) or its 104 times dilution (nutrient-depleted) for seven days. The colony diameters of each strain at the seventh day are shown. Relative diameters of each strain to the nutrient-supplied medium are shown as percentages. Error bars represent S. D. n = 3.CC BY · PubMed Central

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Research works printed but not linked: 26

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Metadata

Authors
Shoji, J.-Y., Kikuma, T., Arioka, M., & Kitamoto, K.
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2010-12-20
Volume
5
Issue
12
Pages
e15650
ISSN
1932-6203
DOI
10.1371/journal.pone.0015650
PMC
PMC3004950
Cited by
80
References
0
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (unspecified)

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