CoChat
Sign Up
Cited by 409

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Piecemeal Microautophagy of Nucleus in Saccharomyces cerevisiae

Roberts, Paul, Moshitch-Moshkovitz, Sharon, Kvam, Erik, O'Toole, Eileen, Winey, Mark, Goldfarb, David S.·2003·Molecular Biology of the Cell
DOI 10.1091/mbc.e02-08-0483
Open this paper in CoChat — free account

Abstract

Nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae are formed through specific interactions between Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope. Herein, we report that NV junctions in yeast promotepiecemeal microautophagy of thenucleus (PMN). During PMN, teardrop-like blebs are pinched from the nucleus, released into the vacuole lumen, and degraded by soluble hydrolases. PMN occurs in rapidly dividing cells but is induced to higher levels by carbon and nitrogen starvation and is under the control of the Tor kinase nutrient-sensing pathway. Confocal and biochemical assays demonstrate that Nvj1p is degraded in a PMN-dependent manner. PMN occurs normally in apg7-Δcells and is, therefore, not dependent on macroautophagy. Transmission electron microscopy reveals that portions of the granular nucleolus are often sequestered into PMN structures. These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.e02-08-0483

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 58

  1. Abeliovich H., 1999, EMBO J., 18, 6005, 10.1093/emboj/18.21.6005 · DOI · Paper page
  2. Abeliovich H., 2000, J. Cell Biol., 151, 1025, 10.1083/jcb.151.5.1025 · DOI
  3. Achleitner G., 1999, Eur. J. Biochem., 264, 545, 10.1046/j.1432-1327.1999.00658.x · DOI
  4. Baba M., 1994, J. Cell Biol., 124, 903, 10.1083/jcb.124.6.903 · DOI
  5. Darsow T., 1997, J. Cell Biol., 138, 517, 10.1083/jcb.138.3.517 · DOI
  6. DeRisi J.L., 1997, Science, 278, 680, 10.1126/science.278.5338.680 · DOI
  7. Fleckenstein D., 1998, J. Cell Sci., 111, 3109, 10.1242/jcs.111.20.3109 · DOI
  8. Guldener U., 1996, Nucleic Acids Res., 24, 2519, 10.1093/nar/24.13.2519 · DOI
  9. Hardwick J.S., 1999, Proc. Natl. Acad. Sci. USA, 96, 14866, 10.1073/pnas.96.26.14866 · DOI
  10. Hutchins M.U., 1999, J. Cell Sci., 112, 4079, 10.1242/jcs.112.22.4079 · DOI
  11. Ichimura Y., 2000, Nature, 408, 488, 10.1038/35044114 · DOI
  12. Jones E.W., 1982, Genetics, 102, 665, 10.1093/genetics/102.4.665 · DOI
  13. Ju Q., 1994, Yeast, 10, 151, 10.1002/yea.320100203 · DOI
  14. Kamada Y., 2000, J. Cell Biol., 150, 1507, 10.1083/jcb.150.6.1507 · DOI · Paper page
  15. Kim J., 2001, J. Cell Biol., 152, 51, 10.1083/jcb.152.1.51 · DOI
  16. Kim J., 2001, J. Biol. Chem., 23, 23
  17. Klionsky D.J., 1999, Annu. Rev. Cell. Dev. Biol., 15, 1, 10.1146/annurev.cellbio.15.1.1 · DOI
  18. Kohlwein S.D., 2001, Mol. Cell. Biol., 21, 109, 10.1128/MCB.21.1.109-125.2001 · DOI
  19. Kremer J.R., 1996, J. Struct. Biol., 116, 71, 10.1006/jsbi.1996.0013 · DOI
  20. Leger-Silvestre I., 1999, Chromosoma, 108, 103, 10.1007/s004120050357 · DOI
  21. 10.1091/mbc.12.6.1633 · DOI
  22. Mizushima N., 1998, Nature, 395, 395, 10.1038/26506 · DOI
  23. Mizushima N., 1998, J. Biol. Chem., 273, 33889, 10.1074/jbc.273.51.33889 · DOI
  24. Moskvina E., 1998, Yeast, 14, 1041, 10.1002/(SICI)1097-0061(199808)14:11<1041::AID-YEA296>3.0.CO;2-4 · DOI
  25. Mukaiyama H., 2002, Genes Cells, 7, 75, 10.1046/j.1356-9597.2001.00499.x · DOI
  26. Muller O., 2000, J. Cell Biol., 151, 519, 10.1083/jcb.151.3.519 · DOI
  27. Noda T., 1995, Biochem. Biophys. Res. Commun., 210, 126, 10.1006/bbrc.1995.1636 · DOI
  28. Noda T., 1998, J. Biol. Chem., 273, 3963, 10.1074/jbc.273.7.3963 · DOI
  29. Noda T., 2002, Trends Cell Biol., 12, 231, 10.1016/S0962-8924(02)02278-X · DOI
  30. Ohsumi Y., 1999, Phil. Trans. R. Soc. Lond. B Biol. Sci., 354, 1577, 10.1098/rstb.1999.0501 · DOI
  31. Pan X., 1998, J. Cell Sci., 111, 2137, 10.1242/jcs.111.15.2137 · DOI
  32. 10.1091/mbc.11.7.2445 · DOI
  33. Pelham H.R., 1984, EMBO J., 3, 3095, 10.1002/j.1460-2075.1984.tb02264.x · DOI
  34. 10.1091/mbc.10.4.987 · DOI
  35. Russell S.J., 1999, J. Biol. Chem., 274, 21943, 10.1074/jbc.274.31.21943 · DOI
  36. Sakai Y., 1998, J. Cell Biol., 141, 625, 10.1083/jcb.141.3.625 · DOI
  37. Sato T.K., 1998, Mol. Cell. Biol., 18, 5308, 10.1128/MCB.18.9.5308 · DOI
  38. Sattler T., 2000, J. Cell Biol., 151, 529, 10.1083/jcb.151.3.529 · DOI
  39. Scott S.V., 1996, Proc. Natl. Acad. Sci. USA, 93, 12304, 10.1073/pnas.93.22.12304 · DOI
  40. Scott S.V., 2000, J. Biol. Chem., 275, 25840, 10.1074/jbc.M002813200 · DOI · Paper page
  41. Severs N.J., 1976, J. Ultrastruct. Res, 54, 374, 10.1016/S0022-5320(76)80023-8 · DOI
  42. Sherman F., 1991, Methods Enzymol., 194, 3, 10.1016/0076-6879(91)94004-V · DOI
  43. Suzuki K., 2001, EMBO J., 20, 5971, 10.1093/emboj/20.21.5971 · DOI
  44. Takeshige K., 1992, J. Cell Biol., 119, 301, 10.1083/jcb.119.2.301 · DOI
  45. 10.1091/mbc.7.1.173 · DOI
  46. Teter S.A., 2000, Semin. Cell. Dev. Biol., 11, 173, 10.1006/scdb.2000.0163 · DOI
  47. Tuttle D.L., 1995, J. Cell Sci., 108, 25, 10.1242/jcs.108.1.25 · DOI · Paper page
  48. van Hoof A., 1999, Cell, 99, 347, 10.1016/S0092-8674(00)81520-2 · DOI
  49. Veit M., 2001, EMBO J., 20, 3145, 10.1093/emboj/20.12.3145 · DOI
  50. Wach A., 1996, Yeast, 12, 259, 10.1002/(SICI)1097-0061(19960315)12:3<259::AID-YEA901>3.0.CO;2-C · DOI
  51. Wang Y.X., 1998, J. Cell Biol., 140, 1063, 10.1083/jcb.140.5.1063 · DOI
  52. 10.1091/mbc.7.9.1375 · DOI
  53. Webster D.L., 1993, Yeast, 9, 1165, 10.1002/yea.320091103 · DOI
  54. Welch W.J., 1986, J. Cell Biol., 103, 2035, 10.1083/jcb.103.5.2035 · DOI
  55. Winey M., 1995, J. Cell Biol., 129, 1601, 10.1083/jcb.129.6.1601 · DOI
  56. Woolford C.A., 1993, J. Biol. Chem., 268, 8990, 10.1016/S0021-9258(18)52969-0 · DOI
  57. 10.1091/mbc.10.5.1353 · DOI
  58. Zubenko G.S., 1983, Proc. Natl. Acad. Sci. USA, 80, 510, 10.1073/pnas.80.2.510 · DOI

Cited by

Sorted by citations

Metadata

Authors
Roberts, P., Moshitch-Moshkovitz, S., Kvam, E., O'Toole, E., Winey, M., & Goldfarb, D. S.
Affiliations
  • Department of Biology, University of Rochester, Rochester, New York 14627;
  • Boulder Laboratory for Three-Dimensional Fine Structure, University of Colorado, Boulder, Colorado 80309-0347; and
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2003-01
Volume
14
Issue
1
Pages
129-141
ISSN
  • 1059-1524
  • 1939-4586
DOI
10.1091/mbc.e02-08-0483
Cited by
409
References
1
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review