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IS-Linked Movement of a Restriction-Modification System
Open accessAbstract
Potential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory condition. After blocking replication of a temperature-sensitive plasmid carrying a PaeR7I restriction-modification system in Escherichia coli cells, the plasmid was found integrated into the chromosome of the surviving cells. Sequence analysis revealed that, in the majority of products, the restriction-modification system was linked to chromosomal insertion sequences (ISs). Three types of products were: (I) apparent co-integration of the plasmid and the chromosome at a chromosomal IS1 or IS5 copy (24/28 analyzed); (II) de novo insertion of IS1 with the entire plasmid except for a 1-3 bp terminal deletion (2/28); and (III) reciprocal crossing-over between the plasmid and the chromosome involving 1-3 bp of sequence identity (2/28). An R-negative mutation apparently decreased the efficiency of successful integration by two orders of magnitude. Reconstruction experiments demonstrated that the restriction-dependence was mainly due to selection against cells without proper integration: their growth was inhibited by the restriction enzyme action. These results demonstrate collaboration of a mobile element and a restriction-modification system for successful joint migration. This collaboration may have promoted the spread and, therefore, the long-term persistence of these complexes and restriction-modification systems in a wide range of prokaryotes.
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LGT involved in RM systems has been widely observed, including insert element-, phage-, and integrative element-mediated transfer.
“LGT involved in the RM systems has been widely observed, including insert element-,37 phage-38, and integrative element-mediated transfer.39”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [37]
Citation 37 is attached to insertion-element-mediated transfer, not the phage or integrative-element examples. The Discussion reports IS-associated movement of an RM gene complex within a genome under laboratory conditions, but describes movement between genomes as an extrapolation rather than an observed result. (LLM, conf=0.85)
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Metadata
- Authors
- Takahashi, N., Ohashi, S., Sadykov, M. R., Mizutani-Ui, Y., & Kobayashi, I.
- Affiliations
- Institute of Medical Science, The University of Tokyo (JP)
- Corresponding author
- Kobayashi, Ichizo
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-01-31
- Volume
- 6
- Issue
- 1
- Pages
- e16554
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3031569
- OpenAlex
- W2024344956
- Cited by
- 18
- References
- 40
- Citations by year
- 2026: 1 · 2022: 1 · 2019: 3 · 2018: 2 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 2
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (19790316, 21370001, 19657002)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
- Keywords
- restriction-modification systems, insertion sequences, IS1, IS5, Escherichia coli, mobile genetic elements, restriction enzymes, temperature-sensitive plasmid, genome rearrangement
- MeSH terms
- Escherichia coli, Plasmids, DNA Restriction-Modification Enzymes, Mutagenesis, Insertional, Genome, Computational Biology
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