Platelet Inhibition by Nitrite Is Dependent on Erythrocytes and Deoxygenation
Open accessAbstract
BACKGROUND: Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet activity was investigated. METHODOLOGY/FINDING: Platelet aggregation was studied in platelet-rich plasma (PRP) and PRP+erythrocytes by turbidimetric and impedance aggregometry, respectively. In PRP, DEANONOate inhibited platelet aggregation induced by ADP while nitrite had no effect on platelets. In PRP+erythrocytes, the inhibitory effect of DEANONOate on platelets decreased whereas nitrite at physiologic concentration (0.1 µM) inhibited platelet aggregation and ATP release. The effect of nitrite+erythrocytes on platelets was abrogated by C-PTIO (a membrane-impermeable NO scavenger), suggesting an NO-mediated action. Furthermore, deoxygenation enhanced the effect of nitrite as observed from a decrease of P-selectin expression and increase of the cGMP levels in platelets. The ADP-induced platelet aggregation in whole blood showed inverse correlations with the nitrite levels in whole blood and erythrocytes. CONCLUSION: Nitrite alone at physiological levels has no effect on platelets in plasma. Nitrite in the presence of erythrocytes inhibits platelets through its reduction to NO, which is promoted by deoxygenation. Nitrite may have role in modulating platelet activity in the circulation, especially during hypoxia.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the context of atherosclerosis, HOCl has been shown to oxidize both high and low density lipoproteins.
“In the context of atherosclerosis, HOCl has been shown to oxidize both high and low density lipoproteins (90, 470, 881, 1042).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [881]
Nitrite decreases platelet activation.
“In addition, nitrite has been shown to decrease platelet activation [11, 12].”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [11]
Nitrite has also been shown to decrease platelet activation and aggregation.
“Nitrite has also been shown to decrease platelet activation and aggregation [11, 12].”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [11]
The effect of nitrite on platelet activation in the presence of erythrocytes was abrogated in the presence of the NO scavenger C-PTIO.
“and abrogated in the presence of the NO scavenger C-PTIO (Figure 7) [115].”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [115]
Erythrocytes can export NO bioactivity from nitrite.
“These data support the notion that erythrocytes can export NO bioactivity from nitrite.”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [11]
The effect of nitrite on platelet activation in the presence of erythrocytes was potentiated upon red cell deoxygenation.
“This effect was potentiated upon red cell deoxygenation”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [115]
In vitro treatment of platelet-rich plasma with nitrite reduces platelet activation in the presence of erythrocytes.
“but reduces platelet activation in the presence of erythrocytes (Figure 7) [115].”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [115]
In vitro treatment of platelet-rich plasma with nitrite has no effect on platelet activation.
“Importantly, in contrast to the effects observed in vivo, the in vitro treatment of platelet rich plasma with nitrite has no effect on platelet activation”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [115]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 34 · Cited by 109
- Authors
- Srihirun, S., Sriwantana, T., Unchern, S., Kittikool, D., Noulsri, E., Pattanapanyasat, K., Fucharoen, S., Piknova, B., Schechter, A. N., & Sibmooh, N.
- Affiliations
- Mahidol University (TH)
- Siriraj Hospital (TH)
- National Institutes of Health (US)
- Corresponding author
- Sibmooh, Nathawut
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-01-20
- Volume
- 7
- Issue
- 1
- Pages
- e30380
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3262819
- OpenAlex
- W2017470603
- Cited by
- 109
- References
- 34
- Citations by year
- 2026: 1 · 2024: 5 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 8 · 2019: 9 · 2018: 15 · 2017: 9 · 2016: 10 · 2015: 10 · 2014: 12 · 2013: 9 · 2012: 5
- FWCI
- 7.68
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Mahidol University
- Thailand Research Fund
- National Institutes of Health
- Office of the Higher Education Commission
- Topics
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Keywords
- nitrite, platelet aggregation, erythrocytes, deoxygenation, nitric oxide, hypoxia, ATP release, P-selectin, cyclic GMP, ADP-induced aggregation, whole blood, platelet function
- MeSH terms
- Adult, Blood Platelets, Cells, Cultured, Erythrocytes, Female, Flow Cytometry, Humans, Male, Nitrites, Oxygen, Platelet Aggregation, Platelet Activation, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
