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NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

Open access
Patel, Ravi K., Jain, Mukesh·2012·PLoS ONE

Abstract

Next generation sequencing (NGS) technologies provide a high-throughput means to generate large amount of sequence data. However, quality control (QC) of sequence data generated from these technologies is extremely important for meaningful downstream analysis. Further, highly efficient and fast processing tools are required to handle the large volume of datasets. Here, we have developed an application, NGS QC Toolkit, for quality check and filtering of high-quality data. This toolkit is a standalone and open source application freely available at http://www.nipgr.res.in/ngsqctoolkit.html. All the tools in the application have been implemented in Perl programming language. The toolkit is comprised of user-friendly tools for QC of sequencing data generated using Roche 454 and Illumina platforms, and additional tools to aid QC (sequence format converter and trimming tools) and analysis (statistics tools). A variety of options have been provided to facilitate the QC at user-defined parameters. The toolkit is expected to be very useful for the QC of NGS data to facilitate better downstream analysis.

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Metadata

Authors
Patel, R. K., & Jain, M.
Venue
PLoS ONE
Published
2012-02-01
Volume
7
Issue
2
Pages
e30619
ISSN
1932-6203 (ISSN-L)
OpenAlex
W1968458420
Cited by
2,917
References
3
FWCI
37.06
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data. https://app.cochat.ai/papers/10.1371/journal.pone.0030619
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Patel, R. K., & Jain, M. (2012). NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data. PLoS ONE. https://doi.org/10.1371/journal.pone.0030619

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