Pharyngeal microflora disruption by antibiotics promotes airway hyperresponsiveness after respiratory syncytial virus infection
Open accessAbstract
BACKGROUND: Regulatory T cells (Treg cells), which are essential for regulation of immune response to respiratory syncytial virus (RSV) infection, are promoted by pharyngeal commensal pneumococcus. The effects of pharyngeal microflora disruption by antibiotics on airway responsiveness and relative immune responses after RSV infection have not been clarified. METHODS: Female BALB/c mice (aged 3 weeks) were infected with RSV and then treated with either oral antibiotics or oral double distilled water (ddH(2)O) from 1 d post infection (pi). Changes in pharyngeal microflora were analyzed after antibiotic treatment for 7 d and 14 d. At 8 d pi and 15 d pi, the inflammatory cells in bronchoalveolar lavage fluid (BALF) were investigated in combination with tests of pulmonary histopathology, airway hyperresponsiveness (AHR), pulmonary and splenic Treg cells responses. Pulmonary Foxp3 mRNA expression, IL-10 and TGF-β1 in BALF and lung homogenate were investigated at 15 d pi. Ovalbumin (OVA) challenge was used to induce AHR after RSV infection. RESULTS: The predominant pharyngeal commensal, Streptococcus, was cleared by antibiotic treatment for 7 d. Same change also existed after antibiotic treatment for 14 d. After RSV infection, AHR was promoted by antibiotic treatment at 15 d pi. Synchronous decreases of pulmonary Treg cells, Foxp3 mRNA and TGF-β1 were detected. Similar results were observed under OVA challenge. CONCLUSIONS: After RSV infection, antibiotic treatment cleared pharyngeal commensal bacteria such as Streptococcus, which consequently, might induce AHR and decrease pulmonary Treg cells.
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The presence of a nasopharyngeal commensal protected mice against RSV-induced airway hyperresponsiveness.
“The presence of a nasopharyngeal commensal protected mice against RSV-induced airway hyperresponsiveness.”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [103]
RSV-infected mice who underwent antibiotic-mediated depletion of Streptococcus viridans in the nasopharynx exhibited increases in number of inflammatory lymphocytes and airway hyperresponsiveness, and decreases in regulatory T cell number and transforming growth factor-β production.
“RSV-infected mice who underwent antibiotic-mediated depletion of Streptococcus viridans in the nasopharynx exhibited increases in number of inflammatory lymphocytes and airway hyperresponsiveness, and decreases in regulatory T cell number and transforming growth factor-β production”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [103]
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- Authors
- Ni, K., Li, S., Xia, Q., Zang, N., Deng, Y., Xie, X., Luo, Z., Luo, Y., Wang, L., Fu, Z., & Liu, E.
- Affiliations
- Chongqing Medical University (CN)
- Children's Hospital of Chongqing Medical University (CN)
- Corresponding author
- Liu, Enmei
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-07-26
- Volume
- 7
- Issue
- 7
- Pages
- e41104
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3406033
- OpenAlex
- W1997538548
- Cited by
- 12
- References
- 41
- Citations by year
- 2021: 2 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2013: 2
- FWCI
- 0.43
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (30972698, 81170010)
- Chongqing Medical University
- Topics
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Keywords
- respiratory syncytial virus infection, airway hyperresponsiveness, antibiotic-induced dysbiosis, pharyngeal microbiota, Streptococcus, regulatory T cells, Foxp3, TGF-β1, IL-10, ovalbumin challenge, allergic airway inflammation
- MeSH terms
- Animals, Anti-Bacterial Agents, Cefoperazone, Female, Gene Expression Regulation, Hypersensitivity, Lung, Mice, Inbred BALB C, Pharynx, Respiratory Syncytial Virus Infections, T-Lymphocytes, Regulatory, Mice, Forkhead Transcription Factors, Transforming Growth Factor beta1, Metagenome
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