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Alginic Acid-Coated Chitosan Nanoparticles Loaded with Legumain DNA Vaccine: Effect against Breast Cancer in Mice
Open accessAbstract
Legumain-based DNA vaccines have potential to protect against breast cancer. However, the lack of a safe and efficient oral delivery system restricts its clinical application. Here, we constructed alginic acid-coated chitosan nanoparticles (A.C.NPs) as an oral delivery carrier for a legumain DNA vaccine. First, we tested its characteristic in acidic environments in vitro. DNA agarose electrophoresis data show that A.C.NPs protected DNA better from degradation in acidic solution (pH 1.5) than did chitosan nanoparticles (C.NPs). Furthermore, size distribution analysis showed that A.C.NPs tended to aggregate and form micrometer scale complexes in pH<2.7, while dispersing into nanoparticles with an increase in pH. Mice were intragastrically administrated A.C.NPs carrying EGFP plasmids and EGFP expression was detected in the intestinal Peyer's patches. Full-length legumain plasmids were loaded into different delivery carriers, including C.NPs, attenuated Salmonella typhimurium and A.C.NPs. A.C.NPs loaded with empty plasmids served as a control. Oral vaccination was performed in the murine orthotopic 4T1 breast cancer model. Our data indicate that tumor volume was significantly smaller in groups using A.C.NPs or attenuated Salmonella typhimurium as carriers. Furthermore, splenocytes co-cultured them with 4T1 cells pre-stimulated with CoCl2, which influenced the translocation of legumain from cytoplasm to plasma membrane, showed a 4.7 and 2.3 folds increase in active cytotoxic T lymphocytes (CD3(+)/CD8(+)/CD25(+)) when treated with A.C.NPs carriers compared with PBS C.NPs. Our study suggests that C.NPs coated with alginic acid may be a safe and efficient tool for oral delivery of a DNA vaccine. Moreover, a legumain DNA vaccine delivered orally with A.C.NPs can effectively improve autoimmune response and protect against breast cancer in mice.
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“It was shown that this vaccine could effectively improve autoimmune response and protect against breast cancer in mice [91].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [91]
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“Alginic acid-coated chitosan nanoparticles have been constructed as an oral delivery carrier for the legumain-based DNA vaccine.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [91]
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“Polysaccharides isolated from various microalgae ranging from diatoms to green-blue algae demonstrated different activities, although direct anticancer properties were rarely reported [91].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [91]
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Metadata
- Authors
- Liu, Z., Lv, D., Liu, S., Gong, J., Wang, D., Xiong, M., Chen, X., Xiang, R., & Tan, X.
- Affiliations
- Nankai University (CN)
- Tianjin University (CN)
- Corresponding author
- Tan, Xiaoyue
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-04-05
- Volume
- 8
- Issue
- 4
- Pages
- e60190
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3618226
- OpenAlex
- W1993169857
- Cited by
- 108
- References
- 37
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 8 · 2022: 8 · 2021: 13 · 2020: 9 · 2019: 7 · 2018: 9 · 2017: 7 · 2016: 13 · 2015: 9 · 2014: 11 · 2013: 7
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- National Natural Science Foundation of China (81273331, 2013CB967201, 973 Program, 81270774)
- National Key Research and Development Program of China (2013CB967201)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- legumain, DNA vaccine, breast cancer, chitosan nanoparticles, oral vaccine delivery, attenuated Salmonella typhimurium, 4T1 breast cancer, cytotoxic T lymphocytes, Peyer's patches, nanoparticle aggregation
- MeSH terms
- Asparaginyl Endopeptidase, Alginates, Animals, Antigen-Presenting Cells, Biological Transport, Cysteine Endopeptidases, Drug Carriers, Female, Gastric Mucosa, Hexuronic Acids, Hydrogen-Ion Concentration, Macrophages, Mammary Neoplasms, Experimental, Mice, Inbred BALB C, Peyer's Patches, T-Lymphocytes, Vaccines, DNA, Cancer Vaccines, Glucuronic Acid, Chitosan, Cell Proliferation, Mice, Nanoparticles
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