Cross-Contamination of a UROtsa Stock with T24 Cells – Molecular Comparison of Different Cell Lines and Stocks
Open accessAbstract
BACKGROUND: UROtsa is an authentic, immortalized human urothelial cell line that is used to study the effects of metals and other toxic substances, mostly in the context of bladder cancer carcinogenesis. Unusual properties on the molecular level of a provided UROtsa cell line stock prompted us to verify its identity. METHODS: UROtsa cell line stocks from different sources were tested on several molecular levels and compared with other cell lines. MicroRNA and mRNA expression was determined by Real-Time PCR. Chromosome numbers were checked and PCR of different regions of the large T-antigen was performed. DNA methylation of RARB, PGR, RASSF1, CDH1, FHIT, ESR1, C1QTNF6, PTGS2, SOCS3, MGMT, and LINE1 was analyzed by pyrosequencing and compared with results from the cell lines RT4, T24, HeLa, BEAS-2B, and HepG2. Finally, short tandem repeat (STR) profiling was applied. RESULTS: All tested UROtsa cell line stocks lacked large T-antigen. STR analysis unequivocally identified our main UROtsa stock as the bladder cancer cell line T24, which was different from two authentic UROtsa stocks that served as controls. Analysis of DNA methylation patterns and RNA expression confirmed their differences. Methylation pattern and mRNA expression of the contaminating T24 cell line showed moderate changes even after long-term culture of up to 56 weeks, whereas miRNAs and chromosome numbers varied markedly. CONCLUSIONS: It is important to check the identity of cell lines, especially those that are not distributed by major cell banks. However, for some cell lines STR profiles are not available. Therefore, new cell lines should either be submitted to cell banks or at least their STR profile determined and published as part of their initial characterization. Our results should help to improve the identification of UROtsa and other cells on different molecular levels and provide information on the use of urothelial cells for long-term experiments.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The bladder cancer cell line T24 was established in 1972 and has contributed to several cell line cross-contamination.
“The bladder cancer cell line T24 was established in 1972 and has contributed to several cell line cross-contamination [20, 21].”
From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [20]
The Discussion says T24 was established in 1973, not 1972. The Discussion and Introduction support the other part of the claim: they describe T24 as a frequent source of cell-line contamination and identify multiple affected lines. (LLM, conf=0.90)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
An integrated encyclopedia of DNA elements in the human genome
The ENCODE Project Consortium (2012) · Nature · doi:10.1038/nature11247 · Cited by 18,072 · see citing works
The epigenetic progenitor origin of human cancer
Feinberg, A. P., OHLSSON, R. I., & Henikoff, S. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1748 · Cited by 1,851 · see citing works
Stýblo, M., Razo, L. M. D., Vega, L., Germolec, D. R., LeCluyse, E. L., Hamilton, G. A., Reed, W., Wang, C., Cullen, W., & Thomas, D. J. (2000) · Archives of Toxicology · doi:10.1007/s002040000134 · Cited by 999 · see citing works
High levels of De Novo methylation and altered chromatin structure at CpG islands in cell lines
Antequera, F., Boyes, J., & Bird, A. P. (1990) · Cell · doi:10.1016/0092-8674(90)90015-7 · Cited by 708 · see citing works
Horowitz, J. M., Park, S. H., Bogenmann, E., Cheng, J., Yandell, D. W., Kaye, F. J., Minna, J. D., Dryja, T. P., & Weinberg, R. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.7.2775 · Cited by 532 · see citing works
Established cell line of urinary bladder carcinoma (T24) containing tumour‐specific antigen
Bubenik, J., Barešová, M., Viklický, V., Jakoubková, J., Sainerová, H., & DONNER, J. (1973) · International Journal of Cancer · doi:10.1002/ijc.2910110327 · Cited by 524 · see citing works
Check your cultures! A list of cross‐contaminated or misidentified cell lines
Capes‐Davis, A., Theodosopoulos, G., Atkin, I., Drexler, H. G., Kohara, A., MacLeod, R. A. F., Masters, J. R., Nakamura, Y., Reid, Y. A., Reddel, R. R., et al. (2010) · International Journal of Cancer · doi:10.1002/ijc.25242 · Cited by 520 · cited 3× · see citing works
“T24 cells are a frequent contaminant that is only ‘outperformed’ by HeLa cells [17].”
BackgroundInfluentialThe Costs of using Unauthenticated, Over-Passaged Cell Lines: How Much more Data do we Need?
Hughes, P., Marshall, D., Reid, Y., Parkes, H., & Gelber, C. (2007) · BioTechniques · doi:10.2144/000112598 · Cited by 434 · see citing works
Maruyama, R., Toyooka, S., Toyooka, K. O., Harada, K., Virmani, A. K., Zöchbauer‐Müller, S., Farinas, A. J., Vakar‐Lopez, F., Minna, J. D., Sagalowsky, A. I., et al. (2001) · PubMed · Cited by 422 · see citing works
Adam, L., Zhong, M., Choi, W., Wei, Q., Nicoloso, M. S., Arora, A., Calin, G. A., Wang, H., Siefker-Radtke, A. O., McConkey, D. J., et al. (2009) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-08-2245 · Cited by 410 · see citing works
Coordinated epigenetic repression of the miR‐200 family and miR‐205 in invasive bladder cancer
Wiklund, E. D., Bramsen, J. B., Hulf, T., Dyrskjøt, L., Ramanathan, R., Hansen, T. B., Villadsen, S. B., Gao, S., Ostenfeld, M. S., Borre, M., et al. (2010) · International Journal of Cancer · doi:10.1002/ijc.25461 · Cited by 367 · see citing works
Uroplakins in urothelial biology, function, and disease
Wu, X., Kong, X., Pellicer, À., Kreibich, G., & Sun, T. (2009) · Kidney International · doi:10.1038/ki.2009.73 · Cited by 353 · see citing works
The Uroepithelium: Not Just a Passive Barrier
Apodaca, G. L. (2003) · Traffic · doi:10.1046/j.1600-0854.2003.00156.x · Cited by 331 · see citing works
Wolff, E. M., Byun, H., Han, H. F., Sharma, S., Nichols, P. W., Siegmund, K. D., Yang, A. S., Jones, P. A., & Liang, G. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1000917 · Cited by 309 · see citing works
Chan, M. W. Y., Chan, L. W., Tang, N. L. S., TONG, J. H. M., Lo, K. W., Lee, T., Cheung, H. Y., Wong, W. S., Chan, P. S. F., Lai, F. M., et al. (2002) · PubMed · Cited by 282 · see citing works
Cell line misidentification: the beginning of the end
American Type Culture Collection Standards Development Organization Workgroup ASN-0002 (2010) · Nature Reviews Cancer · doi:10.1038/nrc2852 · Cited by 266 · see citing works
Taylor, K. H., Kramer, R. S., Davis, J. W., Guo, J., Duff, D. J., Xu, D. H., Caldwell, C. W., & Shi, H. (2007) · Cancer Research · doi:10.1158/0008-5472.can-07-1016 · Cited by 258 · see citing works
Loss of p63 Expression Is Associated with Tumor Progression in Bladder Cancer
Urist, M. J., Como, C. J. D., Lu, M.-L., Charytonowicz, E., Verbel, D., Crum, C. P., Ince, T. A., McKeon, F., & Cordon‐Cardo, C. (2002) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64396-9 · Cited by 256 · see citing works
Excessive CpG island hypermethylation in cancer cell lines versus primary human malignancies
Smiraglia, D. J. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.13.1413 · Cited by 243 · see citing works
Liang, G., Gonzales, F. A., Jones, P. A., Orntoft, T. F., & Thykjær, T. (2002) · PubMed · Cited by 204 · see citing works
Sänchez‐Carbayo, M., Socci, N. D., Charytonowicz, E., Lu, M.-L., Prystowsky, M. B., Childs, G. J., & Cordon‐Cardo, C. (2002) · PubMed · Cited by 197 · see citing works
Simeonova, P. P., Wang, S., Toriuma, W., Kommineni, V., Matheson, J. M., Unimye, N., Kayama, F., Harki, D. A., Ding, M., Vallyathan, V., et al. (2000) · PubMed · Cited by 196 · see citing works
Yang, K., Reddel, R. R., Gerwin, B. I., Miyashita, M., McMenamin, M. G., Lechner, J. F., & Harris, C. C. (1988) · Differentiation · doi:10.1111/j.1432-0436.1988.tb00592.x · Cited by 192 · see citing works
Persistent use of “false” cell lines
Lacroix, M. (2008) · International Journal of Cancer · doi:10.1002/ijc.23233 · Cited by 151 · see citing works
The Immortalized UROtsa Cell Line as a Potential Cell Culture Model of Human Urothelium
Rossi, M. R., Masters, J. R., Park, S., Todd, J. H., Garrett, S. H., Sens, M. A., Somji, S., Nath, J., & Sens, D. A. (2001) · Environmental Health Perspectives · doi:10.1289/ehp.01109801 · Cited by 148 · see citing works
Drobná, Z., Waters, S. B., Devesa, V., Harmon, A. W., Thomas, D. J., & Stýblo, M. (2005) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2004.12.007 · Cited by 135 · see citing works
Epigenetic remodeling during arsenical-induced malignant transformation
Jensen, T. J., Novák, P., Eblin, K. E., Gandolfi, A. J., & Futscher, B. W. (2008) · Carcinogenesis · doi:10.1093/carcin/bgn102 · Cited by 124 · see citing works
Characterization of human uroepithelial cells immortalized in vitro by simian virus 40.
Christian, B. J., Loretz, L. J., Oberley, T. D., & Reznikoff, C. A. (1987) · PubMed · Cited by 114 · see citing works
Inorganic Cadmium- and Arsenite-Induced Malignant Transformation of Human Bladder Urothelial Cells
Sens, D. A. (2004) · Toxicological Sciences · doi:10.1093/toxsci/kfh086 · Cited by 113 · see citing works
Phuchareon, J., Ohta, Y., Woo, J. M., Eisele, D. W., & Tetsu, O. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006040 · Cited by 105 · see citing works
Dirks, W. G., MacLeod, R. A. F., Nakamura, Y., Kohara, A., Reid, Y., Milch, H., Drexler, H. G., & Mizusawa, H. (2010) · International Journal of Cancer · doi:10.1002/ijc.24999 · Cited by 104 · see citing works
Varley, C. L., Stahlschmidt, J., Smith, B. A., Stower, M. J., & Southgate, J. (2004) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)63737-6 · Cited by 104 · see citing works
Immortalisation of human urothelial cells
Petzoldt, J. L., Leigh, I. M., Duffy, P. G., Sexton, C. J., & Masters, J. R. (1995) · Urological Research · doi:10.1007/bf00698738 · Cited by 104 · see citing works
Chan, M. W. Y., Chan, L. W., Tang, N. L. S., Lo, K. W., TONG, J. H. M., Chan, A. W. H., Cheung, H. Y., Wong, W. S., Chan, P. S. F., Lai, F. M., et al. (2003) · International Journal of Cancer · doi:10.1002/ijc.10971 · Cited by 102 · see citing works
CpG island methylation profiling in human melanoma cell lines
Tellez, C. S., Shen, L., Estecio, M. R., Jelı́nek, J., Gershenwald, J. E., & Issa, J. J. (2009) · Melanoma Research · doi:10.1097/cmr.0b013e32832b274e · Cited by 102 · see citing works
Cellular basis of urothelial squamous metaplasia
Liang, F., Bosland, M. C., Huang, H., Romih, R., Baptiste, S. L., Deng, F.-M., Wu, X., Shapiro, E., & Sun, T. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200505035 · Cited by 99 · see citing works
Chapman, E., Hurst, C. D., Pitt, E., Chambers, P. A., Aveyard, J. S., & Knowles, M. A. (2006) · Oncogene · doi:10.1038/sj.onc.1209513 · Cited by 95 · see citing works
Novakovic, B., Gordon, L., Wong, N. C., Moffett, A., Manuelpillai, U., Craig, J. M., Sharkey, A., & Saffery, R. (2011) · Molecular Human Reproduction · doi:10.1093/molehr/gar005 · Cited by 92 · see citing works
Aberrant Promoter Methylation of Multiple Genes during Pathogenesis of Bladder Cancer
Brait, M., Begum, S., Carvalho, A. L., Dasgupta, S., Vettore, A. L., Czerniak, B. A., Caballero, O. L., Westra, W. H., Sidransky, D., & Hoque, M. O. (2008) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-08-0192 · Cited by 79 · see citing works
Gildea, J. J., Golden, W. L., Harding, M. A., & Theodorescu, D. (2000) · Genes Chromosomes and Cancer · doi:10.1002/(sici)1098-2264(200003)27:3 · Cited by 78 · see citing works
End the scandal of false cell lines
Masters, J. R. (2012) · Nature · doi:10.1038/492186a · Cited by 64 · see citing works
RUNX3 Methylation Reveals that Bladder Tumors Are Older in Patients with a History of Smoking
Wolff, E. M., Liang, G., Cortez, C. C., Tsai, Y., Castelao, J. E., Cortessis, V. K., Tsao-Wei, D. D., Groshen, S. G., & Jones, P. A. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-6616 · Cited by 61 · see citing works
Adhim, Z., Matsuoka, T., Bito, T., Shigemura, K., Lee, K.-M., Kawabata, M., Fujisawa, M., Nibu, K., & Shirakawa, T. (2011) · British Journal of Cancer · doi:10.1038/bjc.2011.262 · Cited by 59 · see citing works
Aparicio, A. M., North, B., Barske, L. A., Wang, X., Bollati, V., Weisenberger, D. J., Yoo, C. B., Tannir, N. M., Horne, E., Groshen, S. G., et al. (2009) · Epigenetics · doi:10.4161/epi.4.3.8694 · Cited by 58 · see citing works
Novel ZEB1 expression in bladder tumorigenesis
Kenney, P. A., Wszolek, M. F., Rieger‐Christ, K., Neto, B. S., Gould, J. J., Harty, N. J., Mosquera, J. M., Zeheb, R., Loda, M. F., Darling, D. S., et al. (2010) · British Journal of Urology · doi:10.1111/j.1464-410x.2010.09489.x · Cited by 55 · see citing works
Wnek, S. M., Jensen, T. J., Severson, P. L., Futscher, B. W., & Gandolfi, A. J. (2010) · Toxicological Sciences · doi:10.1093/toxsci/kfq106 · Cited by 52 · see citing works
Escudero‐Lourdes, C., Medeiros, M. K., Cárdenas‐González, M., Wnek, S. M., & Gandolfi, J. A. (2010) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2009.12.029 · Cited by 50 · see citing works
Chen, L., Verity, N., & Chai, K. X. (2009) · BMC Cancer · doi:10.1186/1471-2407-9-377 · Cited by 49 · see citing works
Multiplexed Methylation Profiles of Tumor Suppressor Genes in Bladder Cancer
Cabello, M. J., Grau, L., Franco, N. C., Orenes‐Piñero, E., Alvarez, M., Blanca, A., Zorzo, Ó. H., Palacios, A., Urrutia, M., Gómez, J. M. F., et al. (2011) · Journal of Molecular Diagnostics · doi:10.1016/j.jmoldx.2010.11.008 · Cited by 48 · see citing works
Jensen, T. J., Novák, P., Wnek, S. M., Gandolfi, A. J., & Futscher, B. W. (2009) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2009.08.019 · Cited by 46 · see citing works
Dubé, É., Dufresne, J., Chan, P. T. K., Hermo, L., & Cyr, D. G. (2010) · Biology of Reproduction · doi:10.1095/biolreprod.109.083196 · Cited by 45 · see citing works
Chen, P.-C., Tsai, M., Yip, S. K., Jou, Y., Ng, C., Chen, Y., Wang, X., Huang, W., Tung, C., Chen, G. C. W., et al. (2011) · BMC Medical Genomics · doi:10.1186/1755-8794-4-45 · Cited by 45 · see citing works
False cell lines: The problem and a solution
Masters, J. R. (2002) · Cytotechnology · doi:10.1023/a:1022908930937 · Cited by 43 · see citing works
An hTERT-immortalized human urothelial cell line that responds to anti-proliferative factor
Kim, J., Ji, M., DiDonato, J. A., Rackley, R. R., Kuang, M., Sadhukhan, P. C., Mauney, J. R., Keay, S. K., Freeman, M. R., Liou, L. S., et al. (2010) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-010-9350-y · Cited by 43 · see citing works
Zhu, J., Jiang, Z., Gao, F., Hu, X., Zhou, L., Chen, J., Luo, H., Sun, J., Wu, S., Han, Y., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0028223 · Cited by 40 · see citing works
Kunze, E., Wendt, M., & Schlott, T. (2006) · International Journal of Molecular Medicine · doi:10.3892/ijmm.18.4.547 · Cited by 38 · see citing works
Immortalized human urothelial cells as a model of arsenic-induced bladder cancer
Eblin, K. E., Bredfeldt, T., & Gandolfi, A. J. (2008) · Toxicology · doi:10.1016/j.tox.2008.03.020 · Cited by 30 · see citing works
Salewski, H., Bayer, T. A., Eidhoff, U., Preuß, U., Weggen, S., & Scheidtmann, K. H. (1999) · PubMed · Cited by 29 · see citing works
Database of misidentified cell lines
Freshney, R. I. (2009) · International Journal of Cancer · doi:10.1002/ijc.24998 · Cited by 28 · see citing works
Kato, T., ISHIKAWA, K., Nemoto, R., Senoo, A., & Amano, Y. (1978) · The Tohoku Journal of Experimental Medicine · doi:10.1620/tjem.124.339 · Cited by 28 · see citing works
Urothelial cell transplantation using biodegradable synthetic scaffolds
Scriven, S. D., Trejdosiewicz, L. K., Thomas, D. F. M., & Southgate, J. (2001) · Journal of Materials Science Materials in Medicine · doi:10.1023/a:1012869318205 · Cited by 27 · see citing works
Performance of Survivin mRNA as a Biomarker for Bladder Cancer in the Prospective Study UroScreen
Johnen, G., Gawrych, K., Bontrup, H., Pesch, B., Taeger, D., Banek, S., Kluckert, M., Wellhäußer, H., Eberle, F., Nasterlack, M., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0035363 · Cited by 25 · see citing works
Self-citationda Silva, G. N., Evangelista, A. F., Magalhães, D. A., Macedo, C., Búfalo, M. C., Sakamoto‐Hojo, E. T., Passos, G. A. S., & Salvadori, D. M. F. (2010) · Molecular Biology Reports · doi:10.1007/s11033-010-0536-x · Cited by 25 · see citing works
Eblin, K. E., Jensen, T. J., Wnek, S. M., Buffington, S. E., Futscher, B. W., & Gandolfi, A. J. (2008) · Toxicology · doi:10.1016/j.tox.2008.10.007 · Cited by 25 · see citing works
Identification of Genes Correlated with Early-Stage Bladder Cancer Progression
Stone, R., Sabichi, A. L., Gill, J. R., Lee, I.-L., Adegboyega, P. A., Dai, M. S., Loganantharaj, R., Trutschl, M., Cvek, U., & Clifford, J. L. (2010) · Cancer Prevention Research · doi:10.1158/1940-6207.capr-09-0189 · Cited by 25 · see citing works
Medeiros, M. K., Zheng, X., Novák, P., Wnek, S. M., Chyan, V., Escudero‐Lourdes, C., & Gandolfi, A. J. (2011) · Toxicology · doi:10.1016/j.tox.2011.11.002 · Cited by 22 · see citing works
The Use of Short Tandem Repeat Profiling to Characterize Human Bladder Cancer Cell Lines
Chiong, E., Dadbin, A., Harris, L. D., Sabichi, A. L., & Grossman, H. B. (2009) · Journal of Urology · doi:10.1016/j.juro.2009.01.108 · Cited by 20 · see citing works
Senger, D. R., Perruzzi, C. A., & Ali, I. U. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.14.5107 · Cited by 16 · see citing works
Malkovský, M., & Bubeník (1977) · PubMed · Cited by 15 · see citing works
Growth conditions influence DNA methylation in cultured cerebellar granule cells
Bertino, L., Ruffini, M. C., Copani, A., Bruno, V., Raciti, G., Cambria, A., & Nicoletti, F. (1996) · Developmental Brain Research · doi:10.1016/0165-3806(96)00059-4 · Cited by 9 · see citing works
Expression of AS3MT alters transcriptional profiles in human urothelial cells exposed to arsenite
Hester, S., Drobná, Z., Andrews, D., Liu, J., Waalkes, M., Thomas, D., & Stýblo, M. (2009) · Human & Experimental Toxicology · doi:10.1177/0960327109102368 · Cited by 9 · see citing works
Where have all the cell lines gone?
MacLeod, R. A. F., Dirks, W. G., & Drexler, H. G. (2012) · International Journal of Cancer · doi:10.1002/ijc.27752 · Cited by 6 · see citing works
Gabriel, G. (1994) · Oncology Reports · doi:10.3892/or_00000161 · Cited by 4 · see citing works
Gabriel, U., Giehl, M., Haaß, W., Trojan, L., Michel, M. S., Hofmann, W., Seifarth, W., & Fabarius, A. C. (2010) · Cancer Genetics and Cytogenetics · doi:10.1016/j.cancergencyto.2010.07.135 · Cited by 3 · see citing works
Characterization of the TSU-PR1 cell line by chromosome painting and flow cytometry
Kim, M., Kim, S., Kim, H. J., Hoang, I., Wm, O., Koh, J., Park, H., Jeong, J., Kim, W., Lee, E., et al. (2005) · Cancer Genetics and Cytogenetics · doi:10.1016/j.cancergencyto.2005.04.010 · Cited by 1 · see citing works
Buehring (2004) · doi:10.1290/1543-706x(2004)40
Flagged first, then by citations
1 of the 78 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- [2]WHO (2001) Arsenic and arsenic compunds, Environmental Health Criteria 224. 2nd ed. Geneva: WHO. pp. 501.
- [72]MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, et al (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int J Cancer 83: 555– 563. 10508494
Cited by
Sorted by citations
Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines
Huang, Y., Liu, Y., Zheng, C., & Shen, C. (2017) · PLOS ONE · doi:10.1371/journal.pone.0170384 · Cited by 105 · see citing works
Systematic Review: Characteristics and Preclinical Uses of Bladder Cancer Cell Lines
Zuiverloon, T. C. M., de Jong, F. C., Costello, J. C., & Theodorescu, D. (2018) · Bladder Cancer · doi:10.3233/blc-180167 · Cited by 103 · see citing works
Walter, R. F. H., Rozynek, P., Casjens, S., Werner, R., Mairinger, F. D., Speel, E. M., Hausen, A. Z., Meier, S., Wohlschlaeger, J., Theegarten, D., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0195716 · Cited by 41 · see citing works
Self-citationZhao, F. L., Malm, S. W., Hinchman, A., Cherrington, N. J., Beeks, C. G., & Klimecki, W. T. (2014) · PLoS ONE · doi:10.1371/journal.pone.0114549 · Cited by 25 · see citing works
A Comprehensive Transcriptomic Analysis of Arsenic-Induced Bladder Carcinogenesis
Shukla, V., Chandrasekaran, B., Tyagi, A., Navin, A. K., Saran, U., Adam, R. M., & Damodaran, C. (2022) · Cells · doi:10.3390/cells11152435 · Cited by 20 · see citing works
Biosafety Recommendations on the Handling of Animal Cell Cultures
Herman, P., & Pauwels, K. (2014) · Cell engineering · doi:10.1007/978-3-319-10320-4_22 · Cited by 15 · see citing works
mTORC2 activation is regulated by the urokinase receptor (uPAR) in bladder cancer
Hau, A. M., Leivo, M. Z., Gilder, A. S., Hu, J.-J., Gonias, S. L., & Hansel, D. E. (2016) · Cellular Signalling · doi:10.1016/j.cellsig.2016.10.010 · Cited by 13 · see citing works
Hinrichsen, S., & Planer‐Friedrich, B. (2016) · Environmental Science and Pollution Research · doi:10.1007/s11356-015-5960-y · Cited by 11 · see citing works
Sahu, D., Huan, J., Wang, H., Sahoo, D., Casteel, D. E., Klemke, R. L., Boss, G. R., & Hansel, D. E. (2021) · American Journal Of Pathology · doi:10.1016/j.ajpath.2021.08.002 · Cited by 11 · see citing works
Slusser-Nore, A., Larson‐Casey, J. L., Zhang, R., Zhou, X., Somji, S., Garrett, S. H., Sens, D. A., & Dunlevy, J. R. (2016) · PLoS ONE · doi:10.1371/journal.pone.0147362 · Cited by 8 · see citing works
Johnson, M. (2012) · Materials and Methods · doi:10.13070/mm.en.2.145
Cytotoxic Drugs and their Effects on Bladder Function
Kang, S. H. (2015) · e-publications@bond (Bond University)
An in-house indirect immunofluorescence test for ensuring that the cell bank is contamination free
Keshavarz, M., Shafiee, A., Tariqi, G. R., Izad, M. H., Yousefi, A., & Hosseinzadeh, M. (2015) · Medical Laboratory Sciences · doi:10.22037/amls.v1i3.11512
Zhao, F. L. (2014) · UA Campus Repository (The University of Arizona)
KAHYA, H., & Karaduman, T. (2022) · İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi · doi:10.38079/igusabder.1114239
Hua, X., Zou, R., Bai, X., Yang, Y., Lu, J., & Huang, C. (2023) · BMC Biology · doi:10.1186/s12915-023-01683-2
Genomic Response in Human Urothelial Cells Exposed Chronically to Monomethylarsonous Acid
Medeiros, M. K. (2013) · UA Campus Repository (The University of Arizona)
- Authors
- Johnen, G., Rozynek, P., von der Gathen, Y., Bryk, O., Zdrenka, R., Johannes, C., Weber, D. G., Igwilo-Okuefuna, O., Raiko, I., Hippler, J., Brüning, T., & Dopp, E.
- Affiliations
- Institute for Prevention and Occupational Medicine (DE)
- Essen University Hospital (DE)
- University of Duisburg-Essen (DE)
- Corresponding author
- Johnen, Georg
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-05-17
- Volume
- 8
- Issue
- 5
- Pages
- e64139
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3656924
- OpenAlex
- W2003173831
- Cited by
- 17
- References
- 78
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 1.34
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Surgery › Bladder and Urothelial Cancer Treatments
- Keywords
- T24 cells, cell line cross-contamination, cell line authentication, urothelial cell lines, DNA methylation, microRNA expression, mRNA expression, short tandem repeat profiling, large T antigen, chromosome number, bladder cancer
- MeSH terms
- Antigens, Viral, Tumor, Cell Line, Humans, RNA, Messenger, Sequence Analysis, DNA, Microsatellite Repeats, DNA Methylation, Urothelium, MicroRNAs, Cell Line, Tumor, DNA Contamination, Real-Time Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
