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The Carboxyl-Terminal Amino Acids Render Pro-Human LC3B Migration Similar to Lipidated LC3B in SDS-PAGE
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The appearance of free GFP on western blots can be used to monitor lysis of the inner autophagosome membrane and breakdown of the cargo in metazoans, or the delivery of autophagosomes to, and the breakdown of autophagic bodies within, the fungal and plant vacuole.
“Therefore, the appearance of free GFP on western blots can be used to monitor lysis of the inner autophagosome membrane and breakdown of the cargo in metazoans (Figure 12A) [326,347,348], or the delivery of autophagosomes to, and the breakdown of autophagic bodies within, the fungal [349-351] and plant vacuole [291,292,326,352].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [291]
In Arabidopsis and some mammalian cells, essentially all ATG5 and ATG12 proteins exist in the conjugated form, and expression levels do not change at least during short-term starvation.
“In Arabidopsis and some mammalian cells it appears that essentially all of the ATG5 and ATG12 proteins exist in the conjugated form, and the expression levels do not change, at least during short-term starvation [291, 808, 809, 811].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [291]
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationComprehensive autophagy evaluation in cardiac disease models
Kaludercic, N., Maiuri, M. C., Kaushik, S., Fernández, Á. F., de Bruijn, J., Castoldi, F., Chen, Y., Ito, J., Mukai, R., Murakawa, T., et al. (2020) · Cardiovascular Research · doi:10.1093/cvr/cvz233 · Cited by 64
Metadata
- Authors
- Wang, W., Chen, Z., Billiar, T. R., Stang, M. T., & Gao, W.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-09-10
- Volume
- 8
- Issue
- 9
- Pages
- e74222
- ISSN
- 1932-6203
- PMC
- PMC3769297
- Cited by
- 32
- References
- 1
- Language
- en
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