Cited by 76

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Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes

Seguin-Orlando, Andaine, Schubert, Mikkel, Clary, Joel, Stagegaard, Julia, Alberdi, Maria T., Prado, José Luis, Prieto, Alfredo, Willerslev, Eske, Orlando, Ludovic·2013·PLoS ONE

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Crossref · 10.1371/journal.pone.0078575

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  • Mappers can show different performance depending on the experimental procedure followed during sample preparation, such as the type of DNA library constructed and whether or not extracts were USER-treated.

    “Mappers can show different performance depending on the experimental procedure followed during sample preparation, such as the type of DNA library constructed 185 and whether or not extracts were USER-treated 184 .”

    From Ancient DNA analysis (2021) · cites this paper as [185]

    The Conclusion (page unavailable) says library-preparation methods bias read composition and, together with variation in mappability, affect genomic coverage. The Sequence analysis methods (page unavailable) describe using BWA, but the paper does not compare mappers or show that their performance changes with library type. The separate USER-treatment citation is not assessed here. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 39

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Metadata

Authors
Seguin-Orlando, A., Schubert, M., Clary, J., Stagegaard, J., Alberdi, M. T., Prado, J. L., Prieto, A., Willerslev, E., & Orlando, L.
Venue
PLoS ONE
Published
2013-10-29
Volume
8
Issue
10
Pages
e78575
ISSN
1932-6203
Cited by
76
References
11
Language
en

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