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Cited by 59Pregnancy and preeclampsia studiesReproductive System and PregnancyPrenatal Substance Exposure Effects

Effects of Maternal Smoking on the Placental Expression of Genes Related to Angiogenesis and Apoptosis during the First Trimester

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Kawashima, Akihiro, Koide, Keiko, Ventura, Walter, Hori, Kyoko, Takenaka, Shin, Maruyama, Daisuke, Matsuoka, Ryu, Ichizuka, Kiyotake +1 more·2014·PLoS ONE
DOI 10.1371/journal.pone.0106140PMC PMC4148425
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Abstract

OBJECTIVE: Maternal cigarette smoking is reportedly associated with miscarriage, fetal growth restriction and placental abruption, and is paradoxically associated with a decreased risk of developing preeclampsia. In the present study, we investigated the gene expression levels of villous tissues in early gestation. We compared the expression levels of the genes related to angiogenesis and apoptosis in the villous tissues obtained from smoking and non-smoking pregnant women. MATERIALS AND METHODS: We collected villous tissue samples from 57 women requesting surgical termination due to non-medical reasons at 6-8 weeks of gestation. The maternal cigarette smoking status was evaluated by the level of serum cotinine and patients were divided into active smokers and non-smokers by the serum cotinine level. The placental levels of VEGFA, PGF, FLT1, HIF1A, TP53, BAX and BCL2 mRNA were quantified by real time PCR. RESULTS: The gene expression level of PGF and HIF1A in the active smoker group was significantly higher than that in the non-smoker group. We did not observe any significant differences in the VEGFA or FLT1 expression between the groups. In active smoker group, the gene expression levels of TP53 and BAX were significantly higher than those in the non-smoker group. The ratio of BAX/BCL2 mRNA in the active smoker group was significantly higher than that in the non-smoker group. CONCLUSIONS: Our findings revealed that smoking might affect the placenta during early pregnancy. Maternal cigarette smoking in early pregnancy may be associated with villus hypoxia, which may influence angiogenesis and apoptosis.

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  • Tobacco- and alcohol consumption are two of the most critical teratogens for prenatal development.

    “Tobacco- and alcohol consumption are two of the most critical teratogens for prenatal development [10, 11].”

    From Associations between periconceptional lifestyle behaviours and adverse pregnancy outcomes (2021) · cites this paper as [11]

    The Introduction (page not provided) describes smoking during pregnancy as a major public-health concern and links tobacco exposure to placental and pregnancy complications. The Discussion (page not provided) also describes cigarette-smoke components as pathogenic during pregnancy. The paper does not address alcohol consumption or establish that tobacco and alcohol are two of the most critical prenatal teratogens. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Kawashima, A., Koide, K., Ventura, W., Hori, K., Takenaka, S., Maruyama, D., Matsuoka, R., Ichizuka, K., & Sekizawa, A.
Affiliations
SHOWA Medical University (JP)
Corresponding author
Kawashima, Akihiro
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2014-08-28
Volume
9
Issue
8
Pages
e106140
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0106140
PMC
PMC4148425
OpenAlex
W2039446949
Cited by
59
References
2
Citations by year
2026: 1 · 2025: 4 · 2024: 3 · 2023: 5 · 2022: 7 · 2021: 10 · 2020: 4 · 2019: 2 · 2018: 11 · 2017: 4 · 2016: 4 · 2015: 4
FWCI
4.26
Type
journal article
Language
en
Update notices
expression_of_concern (2023-01-11)
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Smoking Research Foundation
  • Japan Society for the Promotion of Science (26462501)
Topics
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
Keywords
maternal smoking, placental gene expression, angiogenesis, apoptosis, first trimester, placental villi, VEGF-A, Flt-1, HIF1A, prostaglandin F, TP53, Bax, Bcl-2, cotinine, early pregnancy
MeSH terms
Placenta Growth Factor, Adult, Chorionic Villi, Female, Humans, Pregnancy, Pregnancy Proteins, Pregnancy Trimester, First, Smoking, Tumor Suppressor Protein p53, Apoptosis, Gene Expression Regulation, Developmental, Neovascularization, Physiologic, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factor A, bcl-2-Associated X Protein, Hypoxia-Inducible Factor 1, alpha Subunit, Young Adult

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