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Evaluation of Fibroblasts Adhesion and Proliferation on Alginate-Gelatin Crosslinked Hydrogel

Open access
Sarker, Bapi, Singh, Raminder, Silva, Raquel, Roether, Judith A., Kaschta, Joachim, Detsch, Rainer, Schubert, Dirk W., Cicha, Iwona, Boccaccini, Aldo R.·2014·PLoS ONE

Abstract

Due to the relatively poor cell-material interaction of alginate hydrogel, alginate-gelatin crosslinked (ADA-GEL) hydrogel was synthesized through covalent crosslinking of alginate di-aldehyde (ADA) with gelatin that supported cell attachment, spreading and proliferation. This study highlights the evaluation of the physico-chemical properties of synthesized ADA-GEL hydrogels of different compositions compared to alginate in the form of films. Moreover, in vitro cell-material interaction on ADA-GEL hydrogels of different compositions compared to alginate was investigated by using normal human dermal fibroblasts. Viability, attachment, spreading and proliferation of fibroblasts were significantly increased on ADA-GEL hydrogels compared to alginate. Moreover, in vitro cytocompatibility of ADA-GEL hydrogels was found to be increased with increasing gelatin content. These findings indicate that ADA-GEL hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.

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Metadata

Authors
Sarker, B., Singh, R., Silva, R., Roether, J. A., Kaschta, J., Detsch, R., Schubert, D. W., Cicha, I., & Boccaccini, A. R.
Venue
PLoS ONE
Published
2014-09-30
Volume
9
Issue
9
Pages
e107952
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2143637133
Cited by
268
References
1
FWCI
6.41
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Evaluation of Fibroblasts Adhesion and Proliferation on Alginate-Gelatin Crosslinked Hydrogel. https://app.cochat.ai/papers/10.1371/journal.pone.0107952
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Sarker, B., Singh, R., Silva, R., Roether, J. A., Kaschta, J., Detsch, R., Schubert, D. W., Cicha, I., & Boccaccini, A. R. (2014). Evaluation of Fibroblasts Adhesion and Proliferation on Alginate-Gelatin Crosslinked Hydrogel. PLoS ONE. https://doi.org/10.1371/journal.pone.0107952

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