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Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene
Open accessAbstract
Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043) and Photobacterium (p-value: 0.025) were highly represented in fish fed with fishmeal and vegetable-meal diets, respectively. In the stomach samples with the vegetable diet, reads of chloroplasts and mitochondria from vegetable dietary ingredients were rather abundant. Principal Coordinate Analysis showed a clear differentiation between diets in the microbiota present in the gut, supporting the presence of specific bacterial consortia associated with the diet.Although differences in growth and nutritive parameters were not observed, a negative effect of the vegetable diet on the survival rate was determined. Further studies are required to shed more light on the relationship between the immune system and sea bream gastrointestinal tract microbiota and should consider the modulation of the microbiota to improve the survival rate and nutritive efficacy when using plant-based diets.
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Citations
Cites
Role of gastrointestinal microbiota in fish
Nayak, S. K. (2010) · Aquaculture Research · doi:10.1111/j.1365-2109.2010.02546.x · Cited by 1,073 · cited 1×
“Many exogenous and endogenous factors, such as species, age and developmental stage, bacterial colonisation during the larval stage, geographic location, seasonality and other environmental factors, especially temperature, antibiotic use during fish growth, or the individual genetics of each fish can alter the gut mic…”
BackgroundBiesbroek, G., Sanders, E. A. M., Roeselers, G., Wang, X., Caspers, M. P. M., Trzciński, K., Bogaert, D., & Keijser, B. J. F. (2012) · PLoS ONE · doi:10.1371/journal.pone.0032942 · Cited by 196
Cited by
The Gut Microbiota of Marine Fish
Egerton, S., Culloty, S., Whooley, J., Stanton, C., & Ross, R. P. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00873 · Cited by 963
Yukgehnaish, K., Kumar, P., Sivachandran, P., Marimuthu, K., Arshad, A., Paray, B. A., & Arockiaraj, J. (2020) · Reviews in Aquaculture · doi:10.1111/raq.12416 · Cited by 470
Ikeda-Ohtsubo, W., Brugman, S., Warden, C. H., Rebel, J. M. J., Folkerts, G., & Pieterse, C. M. J. (2018) · Frontiers in Nutrition · doi:10.3389/fnut.2018.00090 · Cited by 115
Józefiak, A., Nogales-Mérida, S., Rawski, M., Kierończyk, B., & Mazurkiewicz, J. (2019) · BMC Veterinary Research · doi:10.1186/s12917-019-2070-y · Cited by 93
Ullah, S., Zhang, J., Xu, B., Tegomo, A. F., Sagada, G., Zheng, L., Wang, L., & Shao, Q. (2022) · PLOS ONE · doi:10.1371/journal.pone.0262427 · Cited by 60
Metadata
- Authors
- Estruch, G., Collado, M. C., Peñaranda, D. S., Tomás Vidal, A., Jover Cerdá, M., Pérez Martínez, G., & Martinez-Llorens, S.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-08-28
- Volume
- 10
- Issue
- 8
- Pages
- e0136389
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1916912499
- Cited by
- 191
- References
- 2
- FWCI
- 19.40
- Type
- journal article
- Language
- en
- Open access
- gold
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- CoChat. (2026). Citation check: Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene. https://app.cochat.ai/papers/10.1371/journal.pone.0136389
- The paper
- Estruch, G., Collado, M. C., Peñaranda, D. S., Tomás Vidal, A., Jover Cerdá, M., Pérez Martínez, G., & Martinez-Llorens, S. (2015). Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene. PLOS ONE. https://doi.org/10.1371/journal.pone.0136389
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