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Cited by 23Mesenchymal stem cell researchNeurogenesis and neuroplasticity mechanismsMicroRNA in disease regulation

MiR-124 Promote Neurogenic Transdifferentiation of Adipose Derived Mesenchymal Stromal Cells Partly through RhoA/ROCK1, but Not ROCK2 Signaling Pathway

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Wang, Ye, Wang, Desheng, Guo, Dawen·2016·PLOS ONE
DOI 10.1371/journal.pone.0146646PMC PMC4706435
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Abstract

OBJECTIVE: Some recent studies suggest that multiple miRNAs might regulate neurogenic transdifferentiation of mesenchymal stromal cells (MSCs). In the present study, we hypothesized that the miR-124 can repress the expression of RhoA upon the neurogenesis of adipose derived MSCs (ADMSCs). METHODS: MiRNA expression dynamics during neurogenic transdifferentiation of ADMSCs were measured. The expression of neuron-specific enolase (NSE), Tuj-1 (Neuron-specific class III beta-tubulin) and glial fibrillary acidic protein (GFAP), as well as electrophysiological properties, were detected after neurogenic transdifferentiation. The targeting of miR-124 over RhoA was verified by dual luciferase assay, qRT-PCR and western blot. The functions of miR-124 and the RhoA/ROCK signaling pathway were studied using gain and loss of function experiments in vitro. RESULTS: MiR-124 is significantly upregulated during neurogenic transdifferentiation of ADMSCs. Knockdown of endogenous miR-124 hampered neurogenic transdifferentiation and the acquired electrophysiological properties. MiR-124 could directly target RHOA mRNA and repress its expression, through which it increased the proportion of transdifferentiated (transdiff.) cells with positive NSE, Tuj-1 and GFAP. RhoA/ROCK1, but not ROCK2 is a downstream signaling pathway of miR-124 in the process of transdifferentiation. CONCLUSION: MiR-124 is an important miRNA modulating neurogenic transdifferentiation of ADMSCs at least partly via the miR-124/RhoA/ROCK1 signaling pathway. These findings provided some fundamental information for future use of ADMSCs as an agent for regenerative medicine and cell therapy for neurological diseases.

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  • miR-142 promoted neurogenic differentiation of adipose-derived stem cells by targeting RhoA.

    “Furthermore, miR-142 promoted neurogenic differentiation of adipose-derived stem cells by targeting RhoA [114,115].”

    From RhoA Signaling in Neurodegenerative Diseases (2022) · cites this paper as [115]

    The Results section identifies miR-124—not miR-142—as the microRNA that directly targets RhoA. The Abstract’s Results also attribute the increased neurogenic transdifferentiation of adipose-derived mesenchymal stromal cells to miR-124. (LLM, conf=0.90)

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Cited by

Sorted by citations

Authors
Wang, Y., Wang, D., & Guo, D.
Affiliations
Harbin Medical University (CN)
Corresponding author
Guo, Dawen
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2016-01-08
Volume
11
Issue
1
Pages
e0146646
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0146646
PMC
PMC4706435
OpenAlex
W2231448704
Cited by
23
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 3
FWCI
1.63
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
Keywords
miR-124, adipose-derived mesenchymal stromal cells, RhoA/ROCK1 signaling, ROCK2, microRNA regulation, neuron-specific enolase, Tuj1, glial fibrillary acidic protein, electrophysiological properties
MeSH terms
Adipose Tissue, Animals, Cells, Cultured, Humans, Neurons, RNA, Messenger, Signal Transduction, Up-Regulation, Rats, Sprague-Dawley, Patch-Clamp Techniques, rhoA GTP-Binding Protein, RNA Interference, RNA, Small Interfering, MicroRNAs, Rats, Cell Transdifferentiation, rho-Associated Kinases, Neurogenesis, HEK293 Cells, Mesenchymal Stem Cells, Real-Time Polymerase Chain Reaction

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