Tgm1-like transglutaminases in tilapia (Oreochromis mossambicus)
Open accessAbstract
Among the adaptations of aquatic species during evolution of terrestrial tetrapods was the development of an epidermis preventing desiccation. In present day mammals, keratinocytes of the epidermis, using a membrane-bound transglutaminase (Tgm1), accomplish this function by synthesizing a scaffold of cross-linked protein to which a lipid envelope is attached. This study characterizes the abilities of two homologous transglutaminase isozymes in the teleost fish tilapia to form cross-linked protein structures and their expression in certain tissues. Results indicate they are capable of membrane localization and of generating cellular structures resistant to detergent solubilization. They are both expressed in epithelial cells of the lip, buccal cavity and tips of gill filaments. Adaptation of transglutaminase use in evolution of terrestrial keratinocytes evidently involved refinements in tissue expression, access to suitable substrate proteins and activation of cross-linking during terminal differentiation.
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The transglutaminases (e.g. TGM1 and TGM5) that govern the process of protein cross-linking that produces the SC have been found in fish.
“The transglutaminases (e.g. TGM1 and TGM5) that govern the process of protein cross-linking that produces the SC have been found in fish (Rodriguez Cruz et al., 2017).”
From Genomic and physiological mechanisms underlying skin plasticity during water to air transition in an amphibious fish (2020) · cites this paper as Rodriguez Cruz et al., 2017
The Introduction and Results (page numbers unavailable) identify Tgm1-like enzymes in fish, including two tilapia isozymes capable of protein cross-linking. The paper describes Tgm5 as participating in envelope formation in mammalian epidermis, but does not establish that Tgm5 has been found in fish or that the fish enzymes produce a stratum corneum. (LLM, conf=0.87)
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タンパク質架橋化酵素transglutaminaseのメダカにおけるオルソログとその遺伝子変異体の解析
渡邉, 優. (2020) · Institutional Repositories DataBase (IRDB)
- Authors
- Rodriguez Cruz, S. I., Phillips, M. A., Kültz, D., & Rice, R. H.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Rice, Robert H.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2017-05-04
- Volume
- 12
- Issue
- 5
- Pages
- e0177016
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC5417640
- OpenAlex
- W2611928620
- Cited by
- 10
- References
- 37
- Citations by year
- 2025: 1 · 2024: 2 · 2022: 1 · 2021: 2 · 2020: 4
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- eScholarship (California Digital Library) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- National Institute of Environmental Health Sciences (US) (2 P42 ES04699)
- National Science Foundation (IOS-1355098)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
- Keywords
- transglutaminase, transglutaminase 1, tilapia, Oreochromis mossambicus, crosslinking, epidermis, keratinocyte differentiation, desiccation resistance, epithelial cells, gill filaments, lip, oral cavity, tissue expression, membrane localization
- MeSH terms
- Animals, Transglutaminases, Tilapia, Real-Time Polymerase Chain Reaction
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