Effects of oregano essential oil on the ruminal pH and microbial population of sheep
Open accessAbstract
Oregano essential oil (OEO), which has antimicrobial properties, may be used for altering the ruminal pH and microbial populations of sheep, as observed by the altered volatile fatty acid patterns. To further elucidate the effects of OEO on ruminal pH and microbial populations of sheep, 3 German merino sheep × local sheep crossbred rams with permanent ruminal fistulas were randomly assigned to a 3 × 3 Latin square design with 12-d periods. The treatments were as follows: control (CON); OEO4: OEO supplied at 4 g•d-1; and OEO7: OEO supplied at 7 g•d-1. Starting on day 11, rumen fluid was collected at 0 h, and at 4, 8, 12, 24 and 48 h after supplying OEO, and then pH values of rumen fluid were immediately measured. The abundance of microbial populations was determined by using qPCR. The ruminal pH values were similar among the sheep from all treatments. The abundance of ruminal fungi was higher for the sheep supplied OEO7 compared with the sheep supplied CON and OEO4, especially at 4 and 12 h. The abundance of ruminal protozoa decreased with supplied OEO, indicating that OEO could inhibit the protozoa. The abundance of the total ruminal bacteria was similar for the sheep from all treatments, but R. flavefaciens, R. albus and F. succinogenes increased in the sheep supplied OEO4 compared with those in the sheep supplied CON, however, the sheep supplied OEO7 had higher abundances of R. flavefaciens than the sheep supplied CON. These results demonstrated that supplying OEO to sheep did not affect the ruminal pH but could shift the rumen microbial population to one with less protozoa. Supplying OEO can preferentially enhance the growth of certain rumen microbial populations, but the shifts were influenced by the supply rate. Therefore, supplying low amount (i.e. 4 g•d-1) of OEO could have positive effects on ruminal microbial populations, whereas supplying elevated doses of OEO could be detrimental to those same ruminal microbial populations.
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Supplementation of oregano essential oil at 4 and 7 g/d promoted the population of primary cellulolytic bacteria and ruminal fungi, respectively, in sheep.
“Supplementation of oregano EO at 4 and 7 g/d promoted the population of primary cellulolytic bacteria and ruminal fungi, respectively, in sheep (241).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [241]
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References 59 · Cited by 56
Research works printed but not linked: 2
- [16]Wang J, Wu JP, Jiao T, Zhao SG, Xiao YM, Lei JM. Effects of adding oregano essential oil on sheep rumen fermentation and corn silage and corn straw silage degradation. Gansu Nong Ye Da Xue Xue Bao. 2018; 53: 8– 14.
- [17]NRC. Nutrient requirements of small ruminants. Sheep, goats, cervids, and new world camelids. Natl. Acad. Press, Washington, DC. 2007.
- Authors
- Zhou, R., Wu, J., Zhang, L., Liu, L., Casper, D. P., Jiao, T., Liu, T., Wang, J., Lang, X., Song, S., & Gong, X.
- Affiliations
- Gansu Agricultural University (CN)
- Gansu Academy of Agricultural Sciences (CN)
- Corresponding author
- Wu, Jianping
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2019-05-20
- Volume
- 14
- Issue
- 5
- Pages
- e0217054
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC6527227
- OpenAlex
- W2945672792
- Cited by
- 56
- References
- 59
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 9 · 2022: 13 · 2021: 14 · 2020: 2
- FWCI
- 10.16
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- China’s Natural Science Fund (31460592)
- China’s Agricultural Research system (CARS-39-18)
- Agro-scientific Research in the Public Interest (201503134)
- Gansu Academy of Agricultural Science Fund (2017GAAS30)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- oregano essential oil, rumen pH, rumen microbiota, sheep, rumen protozoa, rumen fungi, rumen bacteria, Ruminococcus flavefaciens, Fibrobacter succinogenes, dose-dependent effects, rumen fluid, volatile fatty acids, antimicrobial feed additives
- MeSH terms
- Animals, Animal Feed, Anti-Infective Agents, Diet, Fatty Acids, Female, Fermentation, Hydrogen-Ion Concentration, Lactation, Male, Oils, Volatile, Plant Oils, RNA, Ribosomal, 16S, Rumen, Sheep, DNA Primers, Dietary Supplements, Origanum
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