The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Stability of circulating microRNAs in serum
Abstract
There is a strong body of evidence by several translational studies which demonstrate the potential of circulating miRNAs as a potential biomarker in oncology. However, recent reports documented varying stability of these small RNA molecules in serum samples. The aim of our pilot study was to evaluate the stability of miRNAs in serum in relation to food intake and sample storage. Serum miRNA expression levels of 16 different miRNAs from 8 healthy volunteers were quantified by real-time PCR. 4 samples from each donor were analysed—2 samples (fasting, in the morning and after food intake, at noon) were analysed within 24h and 2 samples (fasting and after food intake, at noon) were stored at -80°C for 14 days and subsequently analysed. Student´s t-test was used to determine significant differences. The detectability of the distinct miRNA as a surrogate for the stability of these small RNA molecules was slightly altered by the storage conditions, but only a miRNA 22-3p, out of the analysed 16 miRNAs, shows significant lower dCq expression (3.821 vs. 4.530; p<0,01) by qPCR dependent on storage conditions (-80°C vs. 4°C). However, miRNA levels were not affected by food intake. The difference between samples taken in the morning (fasting) and at noon (after a normal meal) did not show any significant differences. MiRNAs can be considered to be a relatively stable tool in laboratory diagnostics, but clearly every new assay needs thorough evaluation. The stability of miRNAs documented here in healthy volunteers shows their potential in the search for innovative biomarkers in oncology.
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“Unlike other RNA species, they are extracellularly stable and circulate in body fluids, including plasma and cerebrospinal fluid (CSF), and therefore, are discussed as biomarkers for various diseases [16–18].”
From Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability (2025) · cites this paper as [17]
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“miRNA is known to be exceptionally stable to degradation in the extracellular space, withstanding even harsh treatments such as numerous freeze-thaw cycles [17, 18].”
From Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability (2025) · cites this paper as [17]
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Cited by
Kumbol, V., Ivanov, A., McGurran, H., Schüler, J., Zhai, Y., Ludwik, K., Hinkelmann, L., Brehm, M., Krüger, C., Küchler, J., et al. (2025) · Cell Communication and Signaling · doi:10.1186/s12964-025-02199-8 · Cited by 8
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Metadata
- Authors
- Kupec, T., Bleilevens, A., Iborra, S., Najjari, L., Wittenborn, J., Maurer, J., & Stickeler, E.
- ORCID
- Kupec, Tomas
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2022-08-31
- Volume
- 17
- Issue
- 8
- Pages
- e0268958
- ISSN
- 1932-6203
- PMC
- PMC9432728
- Cited by
- 60
- References
- 22
- Language
- en
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