Circulating microRNAs as stable blood-based markers for cancer detection
Open accessAbstract
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small ( approximately 22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Extracellular miRNAs are highly stable, resisting degradation at room temperature for up to 4 days and in deleterious conditions such as boiling, multiple freeze-thaw cycles, and high or low pH.
“Contrary to cellular RNA species, extracellular miRNAs are highly stable, resisting degradation at room temperature for up to 4 days and in deleterious conditions such as boiling, multiple freeze-thaw cycles, and high or low pH (117, 124).”
From Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation (2018) · cites this paper as [124]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834 · see citing works
MicroRNA expression profiles classify human cancers
Lü, J., Getz, G. A., Miska, E. A., Alvarez-Saavedra, E., Lamb, J., Peck, D., Sweet-Cordero, A., Ebert, B. L., Mak, R. H., Ferrando, A. A., et al. (2005) · Nature · doi:10.1038/nature03702 · Cited by 9,497 · see citing works
MicroRNA signatures in human cancers
Calin, G. A., & Croce, C. M. (2006) · Nature reviews. Cancer · doi:10.1038/nrc1997 · Cited by 7,592 · see citing works
Oncomirs — microRNAs with a role in cancer
Esquela‐Kerscher, A., & Slack, F. J. (2006) · Nature reviews. Cancer · doi:10.1038/nrc1840 · Cited by 7,038 · see citing works
Real-time quantification of microRNAs by stem-loop RT-PCR
Chen, C. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni178 · Cited by 4,931 · see citing works
miRBase: tools for microRNA genomics
Griffiths‐Jones, S., Saini, H. K., van Dongen, S., & Enright, A. J. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm952 · Cited by 4,349 · see citing works
Protein biomarker discovery and validation: the long and uncertain path to clinical utility
Rifai, N., Gillette, M. A., & Carr, S. A. (2006) · Nature Biotechnology · doi:10.1038/nbt1235 · Cited by 1,867 · see citing works
Lawrie, C. H., Gal, S., Dunlop, H. M., Pushkaran, B., Liggins, A. P., Pulford, K. A. F., Banham, A. H., Pezzella, F., Boultwood, J., Wainscoat, J. S., et al. (2008) · British Journal of Haematology · doi:10.1111/j.1365-2141.2008.07077.x · Cited by 1,739 · see citing works
The Diverse Functions of MicroRNAs in Animal Development and Disease
Kloosterman, W. P., & Plasterk, R. H. A. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.09.009 · Cited by 1,714 · see citing works
MicroRNA Expression in Zebrafish Embryonic Development
Wienholds, E., Kloosterman, W. P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, H. R., Kauppinen, S., & Plasterk, R. H. A. (2005) · Science · doi:10.1126/science.1114519 · Cited by 1,551 · see citing works
Small non-coding RNAs in animal development
Stefani, G., & Slack, F. J. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2347 · Cited by 1,361 · see citing works
Exosomal-like vesicles are present in human blood plasma
Caby, M.-P., Lankar, D., Vincendeau-Scherrer, C., Raposo, G., & Bonnerot, C. (2005) · International Immunology · doi:10.1093/intimm/dxh267 · Cited by 1,348 · see citing works
Etzioni, R., Urban, N. D., Ramsey, S. D., McIntosh, M. W., Schwartz, S., Reid, B. J., Radich, J. P., Anderson, G. L., & Hartwell, L. H. (2003) · Nature reviews. Cancer · doi:10.1038/nrc1041 · Cited by 1,171 · see citing works
Self-citationExosomes: A Common Pathway for a Specialized Function
van Niel, G., Porto-Carreiro, I., Simoes, S., & Raposo, G. (2006) · The Journal of Biochemistry · doi:10.1093/jb/mvj128 · Cited by 922 · see citing works
MicroRNA Expression Profiling in Prostate Cancer
Porkka, K. P., Pfeiffer, M. J., Waltering, K. K., Vessella, R. L., Tammela, T. L. J., & Visakorpi, T. (2007) · Cancer Research · doi:10.1158/0008-5472.can-07-0533 · Cited by 896 · see citing works
Self-citationDetection and Characterization of Placental MicroRNAs in Maternal Plasma
Chim, S. S. C., Shing, T. K. F., Hung, E. C. W., Leung, T.-Y., Lau, T. K., Chiu, R. W. K., & Lo, Y. M. D. (2008) · Clinical Chemistry · doi:10.1373/clinchem.2007.097972 · Cited by 844 · see citing works
Stability of Endogenous and Added RNA in Blood Specimens, Serum, and Plasma
Tsui, N. B. Y., Ng, E. K. O., & Lo, Y. D. (2002) · Clinical Chemistry · doi:10.1093/clinchem/48.10.1647 · Cited by 628 · see citing works
A new human prostate carcinoma cell line, 22Rv1
Sramkoski, R., Pretlow, T. G. I., Giaconia, J. M., Pretlow, T. P., Schwartz, S., Sy, M., Marengo, S. R., Rhim, J. S., Zhang, D.-E., & Jacobberger, J. (1999) · In vitro Cellular & Developmental Biology-Animal · doi:10.1007/s11626-999-0115-4 · Cited by 625 · see citing works
Xi, Y., Nakajima, G., Gavin, E. J., Morris, C. G., Kudo, K., Hayashi, K., & Ju, J. (2007) · RNA · doi:10.1261/rna.642907 · Cited by 567 · see citing works
Li, J., Smyth, P. C., Flavin, R., Cahill, S., Denning, K. M., Aherne, S. T., Guenther, S. M., O’Leary, J. J., & Sheils, O. M. (2007) · BMC Biotechnology · doi:10.1186/1472-6750-7-36 · Cited by 344 · see citing works
El‐Hefnawy, T., Raja, S., Kelly, L. A., Bigbee, W. L., Kirkwood, J. M., Luketich, J. D., & Godfrey, T. E. (2004) · Clinical Chemistry · doi:10.1373/clinchem.2003.028506 · Cited by 273 · see citing works
RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain
NELSON, P. T., BALDWIN, D. A., KLOOSTERMAN, W. P., KAUPPINEN, S., PLASTERK, R. H. A., & MOURELATOS, Z. (2006) · RNA · doi:10.1261/rna.2258506 · Cited by 251 · see citing works
Self-citationNon‐cell autonomous RNA silencing
Voinnet, O. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.09.039 · Cited by 200 · see citing works
Circulating nucleic acids in plasma/serum
Tsang, J. C. H., & Lo, Y. M. D. (2007) · Pathology · doi:10.1080/00313020701230831 · Cited by 179 · see citing works
Systemic RNAi in Caenorhabditis elegansPreprint
Hunter, C. P., Winston, W. M., Molodowitch, C., Feinberg, E. H., Shih, J. D., Sutherlin, M. E., Wright, A. J., & Fitzgerald, M. (2006) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2006.71.060 · Cited by 73 · see citing works
Attardi, B., Burgenson, J., Hild, S. A., & Reel, J. R. (2004) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2004.04.013 · Cited by 60 · see citing works
Tracking Micrometastasis to Multiple Organs with lacZ -tagged CWR22R Prostate Carcinoma Cells
Holleran, J. L., Miller, C. J., & Culp, L. A. (2000) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540004800508 · Cited by 19 · see citing works
Hartel, A., Didier, A., Ulbrich, S. E., et al. (2004) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2004.07.004 · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation
O'Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00402 · Cited by 5,287 · see citing works
Chen, X., Ba, Y., Ma, L., Cai, X., Yin, Y., Wang, K., Guo, J., Zhang, Y., Chen, J., Guo, X., et al. (2008) · Cell Research · doi:10.1038/cr.2008.282 · Cited by 4,560 · see citing works
Arroyo, J. D., Chevillet, J. R., Kroh, E. M., Ruf, I. K., Pritchard, C. C., Gibson, D. F., Mitchell, P. S., Bennett, C. F., Pogosova-Agadjanyan, E. L., Stirewalt, D. L., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1019055108 · Cited by 3,294 · see citing works
Self-citationMicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins
Vickers, K. C., Palmisano, B. T., Shoucri, B. M., Shamburek, R. D., & Remaley, A. T. (2011) · Nature Cell Biology · doi:10.1038/ncb2210 · Cited by 2,741 · see citing works
MicroRNAs in cancer: biomarkers, functions and therapy
Hayes, J., Peruzzi, P. P., & Lawler, S. (2014) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2014.06.005 · Cited by 2,049 · see citing works
Characterization of extracellular circulating microRNA
Turchinovich, A., Weiz, L., Langheinz, A., & Burwinkel, B. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr254 · Cited by 1,941 · see citing works
Cancer-secreted miR-105 destroys vascular endothelial barriers to promote metastasis
Zhou, W., Fong, M. Y., Min, Y., Somlo, G., Liu, L., Palomares, M. R., Yu, Y., Chow, A., O’Connor, S. T. F., Chin, A. R., et al. (2014) · Cancer Cell · doi:10.1016/j.ccr.2014.03.007 · Cited by 1,476 · see citing works
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280 · see citing works
Zhang, L., Hou, D., Chen, X., Li, D., Zhu, L., Zhang, Y., Li, J., Bian, Z., Liang, X., Cai, X., et al. (2012) · Cell Research · doi:10.1038/cr.2011.158 · Cited by 1,216 · see citing works
Circulating microRNAs, potential biomarkers for drug-induced liver injury
Wang, K., Zhang, S., Marzolf, B., Troisch, P., Brightman, A., Hu, Z., Hood, L. E., & Galas, D. J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813371106 · Cited by 1,149 · see citing works
Fong, M. Y., Zhou, W., Liu, L., Alontaga, A. Y., Chandra, M., Ashby, J., Chow, A., O’Connor, S. T. F., Li, S., Chin, A. R., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3094 · Cited by 1,118 · see citing works
Lujambio, A., & Lowe, S. W. (2012) · Nature · doi:10.1038/nature10888 · Cited by 1,084 · see citing works
The majority of microRNAs detectable in serum and saliva is concentrated in exosomes
Gallo, A., Tandon, M., Alevizos, I., & Illei, G. G. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030679 · Cited by 1,060 · see citing works
Detection of ultra-rare mutations by next-generation sequencing
Schmitt, M. W., Kennedy, S. R., Salk, J. J., Fox, E. J., Hiatt, J. B., & Loeb, L. A. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208715109 · Cited by 1,047 · see citing works
Blood Cell Origin of Circulating MicroRNAs: A Cautionary Note for Cancer Biomarker Studies
Pritchard, C. C., Kroh, E., Wood, B., Arroyo, J. D., Dougherty, K. J., Miyaji, M. M., Tait, J. F., & Tewari, M. (2012) · Cancer Prevention Research · doi:10.1158/1940-6207.capr-11-0370 · Cited by 879 · see citing works
Self-citationMicroRNA as Biomarkers and Diagnostics
Wang, J., Chen, J., & Sen, S. (2016) · Journal of Cellular Physiology · doi:10.1002/jcp.25056 · Cited by 793 · see citing works
Yang, M., Chen, J., Su, F., Yu, B., Su, F., Lin, L., Liu, Y., Huang, J.-D., & Song, E. (2011) · Molecular Cancer · doi:10.1186/1476-4598-10-117 · Cited by 693 · see citing works
Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation
Li, D., Liu, J., Guo, B., Liang, C., Dang, L., Lu, C., He, X., Cheung, H. Y.-S., Xu, L., Lu, C., et al. (2016) · Nature Communications · doi:10.1038/ncomms10872 · Cited by 588 · see citing works
The emerging role of cell-free DNA as a molecular marker for cancer management
Bronkhorst, A. J., Ungerer, V., & Holdenrieder, S. (2019) · Biomolecular Detection and Quantification · doi:10.1016/j.bdq.2019.100087 · Cited by 566 · see citing works
Circulating microRNAs as novel biomarkers for diabetes mellitus
Guay, C., & Regazzi, R. (2013) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2013.86 · Cited by 563 · see citing works
Advances in the Early Diagnosis of Hepatocellular Carcinoma
Wang, W., & Wei, C. (2020) · Genes & Diseases · doi:10.1016/j.gendis.2020.01.014 · Cited by 492 · see citing works
Baggish, A. L., Hale, A., Weiner, R. B., Lewis, G. D., Systrom, D., Wang, F., Wang, T. J., & Chan, S. Y. (2011) · The Journal of Physiology · doi:10.1113/jphysiol.2011.213363 · Cited by 431 · see citing works
Interplay between miRNAs and human diseases
Paul, P., Chakraborty, A., Sarkar, D., Langthasa, M., Rahman, M., Bari, M., Singha, R. S., Malakar, A. K., & Chakraborty, S. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.25854 · Cited by 420 · see citing works
Caporali, A., Meloni, M., Völlenkle, C., Bonci, D., Sala-Newby, G. B., Addis, R., Spinetti, G., Losa, S., Masson, R., Baker, A. H., et al. (2011) · Circulation · doi:10.1161/circulationaha.110.952325 · Cited by 397 · see citing works
A Pilot Study of Circulating miRNAs as Potential Biomarkers of Early Stage Breast Cancer
Zhao, H., Shen, J., Medico, L., Wang, D., Ambrosone, C. B., & Liu, S. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013735 · Cited by 398 · see citing works
Role of the tumor microenvironment in tumor progression and the clinical applications (Review)
YUAN, Y., JIANG, Y.-C., SUN, C.-K., & CHEN, Q.-M. (2016) · Oncology Reports · doi:10.3892/or.2016.4660 · Cited by 320 · see citing works
Prada, I., Gabrielli, M., Turola, E., Iorio, A., D’Arrigo, G., Parolisi, R., De Luca, M., Pacifici, M., Bastoni, M., Lombardi, M., et al. (2018) · Acta Neuropathologica · doi:10.1007/s00401-017-1803-x · Cited by 312 · see citing works
The role of microRNAs in endometriosis and associated reproductive conditions
Ohlsson Teague, E. M. C., Print, C. G., & Hull, M. L. (2010) · Human Reproduction Update · doi:10.1093/humupd/dmp034 · Cited by 309 · see citing works
miR-21: a non‐specific biomarker of all maladies
Jenike, A. E., & Halushka, M. K. (2021) · Biomarker Research · doi:10.1186/s40364-021-00272-1 · Cited by 248 · see citing works
Nongenetic inheritance and transgenerational epigenetics
Szyf, M. (2015) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2014.12.004 · Cited by 238 · see citing works
MicroRNAs: biomarkers, diagnostics, and therapeutics
Huang, W. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7046-9_4 · Cited by 237 · see citing works
Circulating miRNAs as Potential Biomarkers in Alzheimer's Disease
Galimberti, D., Villa, C., Fenoglio, C., Serpente, M., Ghezzi, L., Cioffi, S. M. G., Arighi, A., Fumagalli, G., & Scarpini, E. (2014) · Journal of Alzheimer’s Disease · doi:10.3233/jad-140756 · Cited by 222 · see citing works
Hannafon, B. N., Carpenter, K. J., Berry, W. L., Janknecht, R., Dooley, W. C., & Ding, W.-Q. (2015) · Molecular Cancer · doi:10.1186/s12943-015-0400-7 · Cited by 218 · see citing works
Diverse human extracellular RNAs are widely detected in human plasma
Freedman, J. E., Gerstein, M., Mick, E., Rozowsky, J., Levy, D., Kitchen, R., Das, S., Shah, R., Danielson, K., Beaulieu, L., et al. (2016) · Nature Communications · doi:10.1038/ncomms11106 · Cited by 202 · see citing works
Spatial sorting enables comprehensive characterization of liver zonation
Ben-Moshe, S., Shapira, Y., Moor, A. E., Halpern, K. B., & Itzkovitz, S. (2019) · doi:10.1101/529784 · Cited by 205 · see citing works
Spatial sorting enables comprehensive characterization of liver zonation
Ben-Moshe, S., Shapira, Y., Moor, A. E., Manco, R., Veg, T., Bahar Halpern, K., & Itzkovitz, S. (2019) · Nature Metabolism · doi:10.1038/s42255-019-0109-9 · Cited by 205 · see citing works
Plasma MicroRNAs as Novel Biomarkers for Endometriosis and Endometriosis-Associated Ovarian Cancer
Suryawanshi, S., Vlad, A. M., Lin, H.-M., Mantia-Smaldone, G., Laskey, R., Lee, M., Lin, Y., Donnellan, N., Klein-Patel, M., Lee, T., et al. (2013) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-12-2726 · Cited by 197 · see citing works
MicroRNAs in placental health and disease
Mouillet, J.-F., Ouyang, Y., Coyne, C. B., & Sadovsky, Y. (2015) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2015.05.057 · Cited by 195 · see citing works
Wang, W.-T., Zhao, Y.-N., Han, B.-W., Hong, S.-J., & Chen, Y.-Q. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2012-2415 · Cited by 193 · see citing works
Peripheral whole blood microRNA alterations in major depression and bipolar disorder
Maffioletti, E., Cattaneo, A., Rosso, G., Maina, G., Maj, C., Gennarelli, M., Tardito, D., & Bocchio-Chiavetto, L. (2016) · Journal of Affective Disorders · doi:10.1016/j.jad.2016.04.021 · Cited by 181 · see citing works
Circulating microRNAs as potential biomarkers for endometriosis
Cho, S., Mutlu, L., Grechukhina, O., & Taylor, H. S. (2015) · Fertility and Sterility · doi:10.1016/j.fertnstert.2015.02.013 · Cited by 181 · see citing works
Plasma miR-17-5p, miR-20a and miR-22 are down-regulated in women with endometriosis
Jia, S.-Z., Yang, Y., Lang, J., Sun, P., & Leng, J. (2013) · Human Reproduction · doi:10.1093/humrep/des413 · Cited by 172 · see citing works
MicroRNA profiling of adrenocortical tumors reveals miR-483 as a marker of malignancy
Patterson, E. E., Holloway, A. K., Weng, J., Fojo, T., & Kebebew, E. (2011) · Cancer · doi:10.1002/cncr.25724 · Cited by 171 · see citing works
Toward the promise of microRNAs - Enhancing reproducibility and rigor in microRNA research
Witwer, K. W., & Halushka, M. K. (2016) · RNA Biology · doi:10.1080/15476286.2016.1236172 · Cited by 151 · see citing works
Bhome, R., Goh, R. W., Bullock, M. D., Pillar, N., Thirdborough, S. M., Mellone, M., Mirnezami, R., Galea, D., Veselkov, K., Gu, Q., et al. (2017) · Aging · doi:10.18632/aging.101355 · Cited by 155 · see citing works
Toward the human cellular microRNAome
McCall, M. N., Kim, M.-S., Adil, M., Patil, A. H., Lu, Y., Mitchell, C. J., Leal-Rojas, P., Xu, J., Kumar, M., Dawson, V. L., et al. (2017) · Genome Research · doi:10.1101/gr.222067.117 · Cited by 151 · see citing works
Fassbender, A., Vodolazkaia, A., Saunders, P., Lebovic, D., Waelkens, E., De Moor, B., & D'Hooghe, T. (2013) · Fertility and Sterility · doi:10.1016/j.fertnstert.2013.01.097 · Cited by 150 · see citing works
Hudson, M. B., Woodworth-Hobbs, M. E., Zheng, B., Rahnert, J. A., Blount, M. A., Gooch, J. L., Searles, C. D., & Price, S. R. (2014) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00266.2013 · Cited by 137 · see citing works
Ramirez-Garrastacho, M., Bajo-Santos, C., Line, A., Martens-Uzunova, E. S., de la Fuente, J. M., Moros, M., Soekmadji, C., Tasken, K. A., & Llorente, A. (2022) · British Journal of Cancer · doi:10.1038/s41416-021-01610-8 · Cited by 130 · see citing works
Li, Y., Dalli, J., Chiang, N., Baron, R. M., Quintana, C., & Serhan, C. N. (2013) · Immunity · doi:10.1016/j.immuni.2013.10.011 · Cited by 126 · see citing works
microRNAs expression in endometriosis and their relation to angiogenic factors
Ramon, L. A., Braza-Boils, A., Gilabert-Estelles, J., Gilabert, J., Espana, F., Chirivella, M., & Estelles, A. (2011) · Human Reproduction · doi:10.1093/humrep/der025 · Cited by 117 · see citing works
Research progress of CTC, ctDNA, and EVs in cancer liquid biopsy
Wang, X., Wang, L., Lin, H., Zhu, Y., Huang, D., Lai, M., Xi, X., Huang, J., Zhang, W., & Zhong, T. (2024) · Frontiers in Oncology · doi:10.3389/fonc.2024.1303335 · Cited by 112 · see citing works
Iftikhar, H., & Carney, G. E. (2016) · BioEssays · doi:10.1002/bies.201500130 · Cited by 87 · see citing works
Circulating microRNAs as biomarkers for depression: Many candidates, few finalists
Yuan, H., Mischoulon, D., Fava, M., & Otto, M. W. (2018) · Journal of Affective Disorders · doi:10.1016/j.jad.2017.06.058 · Cited by 86 · see citing works
Identification of aberrant circulating mi<scp>RNA</scp>s in Parkinson's disease plasma samples
Chen, L., Yang, J., Lü, J., Cao, S., Zhao, Q., & Yu, Z. (2018) · Brain and Behavior · doi:10.1002/brb3.941 · Cited by 85 · see citing works
“…information Tianjin Health Bureau Research Fund, Grant/ Award Number: 2013KR12 K E Y W O R D S circulating miRNAs, diagnosis, Parkinson’s disease In the past years, miRNAs were found to be present in body fluids including blood, plasma, serum, saliva, urine, and milk (Mitchell et al., 2008).”
BackgroundSoliman, H. M., Ghonaim, G. A., Gharib, S. M., Chopra, H., Farag, A. K., Hassanin, M. H., Nagah, A., Emad-Eldin, M., Hashem, N. E., Yahya, G., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910794 · Cited by 81 · see citing works
He, S., Huang, L., Shao, C., Nie, T., Xia, L., Cui, B., Lu, F., Zhu, L., Chen, B., & Yang, Q. (2021) · Translational Neurodegeneration · doi:10.1186/s40035-021-00249-y · Cited by 80 · see citing works
Du, L., Schageman, J. J., Irnov, Girard, L., Hammond, S. M., Minna, J. D., Gazdar, A. F., & Pertsemlidis, A. (2010) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/1756-9966-29-75 · Cited by 73 · see citing works
Kim, S.-M., Lim, S.-M., Yoo, J.-A., Woo, M.-J., & Cho, K.-H. (2015) · Food & Function · doi:10.1039/c5fo00738k · Cited by 69 · see citing works
Noncoding RNAs regulate NF-κB signaling to modulate blood vessel inflammation
Cheng, H. S., Njock, M.-S., Khyzha, N., Dang, L. T., & Fish, J. E. (2014) · Frontiers in Genetics · doi:10.3389/fgene.2014.00422 · Cited by 69 · see citing works
Laudanski, P., Charkiewicz, R., Kuzmicki, M., Szamatowicz, J., Charkiewicz, A., & Niklinski, J. (2013) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-11-78 · Cited by 70 · see citing works
MicroRNAs and miRceptors: a new mechanism of action for intercellular communication
Fabbri, M. (2018) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2016.0486 · Cited by 65 · see citing works
Validation of differentially expressed brain‐enriched microRNAs in the plasma of PD patients
Ravanidis, S., Bougea, A., Papagiannakis, N., Koros, C., Simitsi, A. M., Pachi, I., Breza, M., Stefanis, L., & Doxakis, E. (2020) · Annals of Clinical and Translational Neurology · doi:10.1002/acn3.51146 · Cited by 63 · see citing works
Circulating MicroRNAs as Minimally Invasive Biomarkers for Cancer Theragnosis and Prognosis
Cho, W. C. S. (2011) · Frontiers in Genetics · doi:10.3389/fgene.2011.00007 · Cited by 61 · see citing works
Kim, K. T., Angerani, S., Chang, D., & Winssinger, N. (2019) · Journal of the American Chemical Society · doi:10.1021/jacs.9b05688 · Cited by 52 · see citing works
Circulating microRNA profile throughout the menstrual cycle
Rekker, K., Saare, M., Roost, A. M., Salumets, A., & Peters, M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0081166 · Cited by 52 · see citing works
Noninvasive biomarkers of endometriosis: myth or reality?
Lanišnik Rižner, T. (2014) · Expert Review of Molecular Diagnostics · doi:10.1586/14737159.2014.899905 · Cited by 52 · see citing works
Analysis of miRNA expression profiles in breast cancer using biclustering
Fiannaca, A., La Rosa, M., La Paglia, L., Rizzo, R., & Urso, A. (2015) · BMC Bioinformatics · doi:10.1186/1471-2105-16-s4-s7 · Cited by 45 · see citing works
miRNAs as therapeutic tools and biomarkers for prostate cancer
Arrighetti, N., & Beretta, G. L. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13030380 · Cited by 43 · see citing works
Defining the prognostic role of MicroRNAs in cutaneous melanoma
Dika, E., Riefolo, M., Porcellini, E., Broseghini, E., Ribero, S., Senetta, R., Osella-Abate, S., Scarfì, F., Lambertini, M., Veronesi, G., et al. (2020) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2020.03.949 · Cited by 39 · see citing works
Garavelli, S., Bruzzaniti, S., Tagliabue, E., Di Silvestre, D., Prattichizzo, F., Mozzillo, E., Fattorusso, V., La Sala, L., Ceriello, A., Puca, A. A., et al. (2020) · Diabetologia · doi:10.1007/s00125-020-05237-x · Cited by 37 · see citing works
Circulating Level of miR-378 Predicts Left Ventricular Hypertrophy in Patients with Aortic Stenosis
Chen, Z., Li, C., Xu, Y., Li, Y., Yang, H., & Rao, L. (2014) · PLoS ONE · doi:10.1371/journal.pone.0105702 · Cited by 36 · see citing works
Monnaka, V. U., Hernandes, C., Heller, D., & Podgaec, S. (2021) · Einstein (São Paulo) · doi:10.31744/einstein_journal/2021rw5704 · Cited by 33 · see citing works
Extracellular <scp>RNA</scp> in aging
Dluzen, D. F., Noren Hooten, N., & Evans, M. K. (2017) · WIREs RNA · doi:10.1002/wrna.1385 · Cited by 31 · see citing works
A curated human cellular microRNAome based on 196 primary cell types
Patil, A. H., Baran, A., Brehm, Z. P., McCall, M. N., & Halushka, M. K. (2022) · GigaScience · doi:10.1093/gigascience/giac083 · Cited by 30 · see citing works
Yoon, H., Belmonte, K. C., Kasten, T., Bateman, R., & Kim, J. (2017) · Scientific Reports · doi:10.1038/s41598-017-13031-w · Cited by 26 · see citing works
MicroRNAs as Regulators of Phagocytosis
Gierlikowski, W., & Gierlikowska, B. (2022) · Cells · doi:10.3390/cells11091380 · Cited by 25 · see citing works
Radiogenomics predicts the expression of microRNA-1246 in the serum of esophageal cancer patients
Hoshino, I., Yokota, H., Ishige, F., Iwatate, Y., Takeshita, N., Nagase, H., Uno, T., & Matsubara, H. (2020) · Scientific Reports · doi:10.1038/s41598-020-59500-7 · Cited by 19 · see citing works
Old problem, new solutions: biomarker discovery for acetaminophen liver toxicity
Gloor, Y., Schvartz, D., & F.Samer, C. (2019) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1080/17425255.2019.1642323 · Cited by 18 · see citing works
Kumbol, V., Ivanov, A., McGurran, H., Schüler, J., Zhai, Y., Ludwik, K., Hinkelmann, L., Brehm, M., Krüger, C., Küchler, J., et al. (2025) · Cell Communication and Signaling · doi:10.1186/s12964-025-02199-8 · Cited by 8 · see citing works
ATF1 and miR-27b-3p drive intervertebral disc degeneration through the PPARG/NF-κB signaling axis
Guo, W., Mu, K., Geng, J.-C., Xing, H.-Y., Dong, Y., Liu, W.-D., Wang, S.-C., Shi, J.-X., Xing, B.-R., Zhao, J.-Y., et al. (2025) · Communications Biology · doi:10.1038/s42003-025-08186-6 · Cited by 7 · see citing works
- Authors
- Mitchell, P. S., Parkin, R. K., Kroh, E. M., Fritz, B. R., Wyman, S. K., Pogosova-Agadjanyan, E. L., Peterson, A., Noteboom, J., O'Briant, K. C., Allen, A., Lin, D. W., Urban, N., Drescher, C. W., Knudsen, B. S., Stirewalt, D. L., Gentleman, R., Vessella, R. L., Nelson, P. S., Martin, D. B., & Tewari, M.
- Affiliations
- Fred Hutch Cancer Center (US)
- Illumina (United States) (US)
- Clinical Research, and
- University of Washington (US)
- Institute for Systems Biology (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2008-07-29
- Volume
- 105
- Issue
- 30
- Pages
- 10513-10518
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2492472
- OpenAlex
- W2115093090
- Cited by
- 7,969
- References
- 28
- Citations by year
- 2026: 202 · 2025: 252 · 2024: 270 · 2023: 392 · 2022: 409 · 2021: 513 · 2020: 564 · 2019: 548 · 2018: 564 · 2017: 586 · 2016: 621 · 2015: 693 · 2014: 646 · 2013: 599 · 2012: 446
- FWCI
- 74.94
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fondation pour la Recherche Médicale
- National Institutes of Health (5p50ca083636-15)
- National Cancer Institute (P50-CA083636, CA 83636, P50 CA83636, P01CA085859, P50-CA97186, P50CA097186)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30-DK56465, P30DK056465)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Keywords
- circulating microRNAs, cancer detection, miR-141, prostate cancer, xenografts, serum biomarkers
- MeSH terms
- Animals, Cloning, Molecular, Humans, Male, Neoplasm Transplantation, Neoplasms, Prostatic Neoplasms, Ribonucleases, RNA, Neoplasm, Sensitivity and Specificity, Biomarkers, Tumor, Gene Expression Regulation, Neoplastic, Gene Expression Profiling, MicroRNAs, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
