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Cited by 2Helicobacter pylori-related gastroenterology studiesBacterial biofilms and quorum sensingGastric Cancer Management and Outcomes

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Identification of interaction partners of outer inflammatory protein A: Computational and experimental insights into how Helicobacter pylori infects host cells

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Akcelik-Deveci, Sümeyye, Kılıç, Elif, Mansur-Ozen, Nesteren, Timucin, Emel, Buyukcolak, Yaren, Oktem-Okullu, Sinem·2024·PLOS ONE
DOI 10.1371/journal.pone.0300557PMC PMC11521304

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Abstract

Outer membrane proteins (OMPs) play a key role in facilitating the survival of Helicobacter pylori within the gastric tissue by mediating adherence. Among these proteins, Outer inflammatory protein A (OipA) is a critical factor in H . pylori colonization of the host gastric epithelial cell surface. While the role of OipA in H . pylori attachment and its association with clinical outcomes have been established, the structural mechanisms underlying OipA’s action in adherence to gastric epithelial cells remain limited. Our study employed experimental and computational approaches to investigate the interaction partners of OipA on the gastric epithelial cell surface. Initially, we conducted a proteomic analysis using a pull-down assay with recombinant OipA and gastric epithelial cell membrane proteins to identify the OipA interactome. This analysis revealed 704 unique proteins that interacted with OipA. We subsequently analyzed 16 of these OipA partners using molecular modeling tools. Among these 16 partners, we highlight three human proteins, namely Hepatocyte growth factor (HGF), Mesenchymal epithelial transition factor receptor (Met), and Adhesion G Protein-Coupled Receptor B1 (AGRB1) that could play a role in H . pylori adherence to the gastric epithelial cell surface with OipA. Collectively, these findings reveal novel host interactions mediated by OipA, suggesting their potential as therapeutic targets for combating H . pylori infection.

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Metadata

Authors
Akcelik-Deveci, S., Kılıç, E., Mansur-Ozen, N., Timucin, E., Buyukcolak, Y., & Oktem-Okullu, S.
Affiliations
Acıbadem University (TR)
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2024-10-29
Volume
19
Issue
10
Pages
e0300557
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0300557
PMC
PMC11521304
OpenAlex
W4403863659
Cited by
2
References
4
Citations by year
2026: 2
FWCI
0.84
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Ulusal Metroloji Enstitüsü, Türkiye Bilimsel ve Teknolojik Araştirma Kurumu (the project number 217S384)
Topics
  • Health Sciences › Medicine › Gastroenterology › Helicobacter pylori-related gastroenterology studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Health Sciences › Medicine › Oncology › Gastric Cancer Management and Outcomes
Keywords
Helicobacter pylori, oipA, outer membrane proteins, host-pathogen interaction, protein-protein interaction, gastric epithelial cells, bacterial adhesion, hepatocyte growth factor, c-Met, therapeutic target
MeSH terms
Bacterial Adhesion, Bacterial Outer Membrane Proteins, Epithelial Cells, Gastric Mucosa, Humans, Protein Binding, Helicobacter pylori, Helicobacter Infections, Hepatocyte Growth Factor, Proto-Oncogene Proteins c-met, Proteomics, Host-Pathogen Interactions

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