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Cited by 276

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages

Open access
Welsch, Sonja, Keppler, Oliver T, Habermann, Anja, Allespach, Ina, Krijnse-Locker, Jacomine, Kräusslich, Hans-Georg·2007·PLoS Pathogens
DOI 10.1371/journal.ppat.0030036
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Abstract

HIV-1 assembly and release are believed to occur at the plasma membrane in most host cells with the exception of primary macrophages, for which exclusive budding at late endosomes has been reported. Here, we applied a novel ultrastructural approach to assess HIV-1 budding in primary macrophages in an immunomarker-independent manner. Infected macrophages were fed with BSA-gold and stained with the membrane-impermeant dye ruthenium red to identify endosomes and the plasma membrane, respectively. Virus-filled vacuolar structures with a seemingly intracellular localization displayed intense staining with ruthenium red, but lacked endocytosed BSA-gold, defining them as plasma membrane. Moreover, HIV budding profiles were virtually excluded from gold-filled endosomes while frequently being detected on ruthenium red-positive membranes. The composition of cellular marker proteins incorporated into HIV-1 supported a plasma membrane-derived origin of the viral envelope. Thus, contrary to current opinion, the plasma membrane is the primary site of HIV-1 budding also in infected macrophages.

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Metadata

Authors
Welsch, S., Keppler, O. T., Habermann, A., Allespach, I., Krijnse-Locker, J., & Kräusslich, H.-G.
Venue
PLoS Pathogens
Publisher
Public Library of Science (PLoS)
Published
2007-03-23
Volume
3
Issue
3
Pages
e36
ISSN
  • 1553-7366 (ISSN-L)
  • 1553-7374
DOI
10.1371/journal.ppat.0030036
OpenAlex
W1991586131
Cited by
276
References
44
FWCI
10.85
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages. https://app.cochat.ai/papers/10.1371/journal.ppat.0030036
The paper
Welsch, S., Keppler, O. T., Habermann, A., Allespach, I., Krijnse-Locker, J., & Kräusslich, H.-G. (2007). HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages. PLoS Pathogens. https://doi.org/10.1371/journal.ppat.0030036

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.