The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Increased Integrity of Free Circulating DNA in Sera of Patients with Colorectal or Periampullary Cancer: Direct Quantitative PCR for ALU Repeats
Abstract
Background: Cell-free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic nondiseased cells, DNA released from dead cancer cells varies in size. We developed a novel method to measure the ratio of longer to shorter DNA fragments (DNA integrity) in serum as a potential biomarker for patients with colorectal cancer (CRC) or periampullary cancers (PACs). Methods: Sera from 32 patients with CRC (3 stage I, 14 stage II, 6 stage III, and 9 stage IV patients), 19 patients with PACs (2 stage I, 9 stage II, 1 stage III, and 7 stage IV patients), and 51 healthy volunteers were assessed by quantitative real-time PCR of ALU repeats (ALU-qPCR) with 2 sets of primers (115 and 247 bp) amplifying different lengths of DNA. We used serum directly as a template for ALU-qPCR without DNA purification. DNA integrity was determined as ratio of qPCR results of 247-bp ALU over 115-bp ALU. Results: ALU-qPCR had a detection limit of 0.01 pg of DNA. Eliminating DNA purification reduced technical artifacts and reagent/labor costs. Serum DNA integrity was significantly increased for stage I/II and III/IV CRC and stage I/II and III/IV PACs (P = 0.002, P = 0.006, P = 0.022, and P <0.0001, respectively). ROC curves for detecting CRC and PACs had areas under the curves of 0.78 and 0.80, respectively. Conclusions: Direct ALU-qPCR is a robust, highly sensitive, and high-throughput method to measure serum DNA integrity. DNA integrity is a potential serum biomarker for detection and evaluation of CRC and PACs.
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Giacona, M. B., Ruben, G. C., Iczkowski, K. A., Roos, T. B., Porter, D. M., & Sorenson, G. D. (1998) · Pancreas · doi:10.1097/00006676-199807000-00012 · Cited by 387
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Circulating Tumor DNA as a Liquid Biopsy for Cancer
Heitzer, E., Ulz, P., & Geigl, J. B. (2015) · Clinical Chemistry · doi:10.1373/clinchem.2014.222679 · Cited by 767
The emerging role of cell-free DNA as a molecular marker for cancer management
Bronkhorst, A. J., Ungerer, V., & Holdenrieder, S. (2019) · Biomolecular Detection and Quantification · doi:10.1016/j.bdq.2019.100087 · Cited by 566
Direct Serum Assay for MicroRNA-21 Concentrations in Early and Advanced Breast Cancer
Asaga, S., Kuo, C., Nguyen, T., Terpenning, M., Giuliano, A. E., & Hoon, D. S. (2011) · Clinical Chemistry · doi:10.1373/clinchem.2010.151845 · Cited by 451
Self-citationEffect of DNase and Antibiotics on Biofilm Characteristics
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Metadata
- Authors
- Umetani, N., Kim, J., Hiramatsu, S., Reber, H. A., Hines, O. J., Bilchik, A. J., & Hoon, D. S. B.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-06-01
- Volume
- 52
- Issue
- 6
- Pages
- 1062-1069
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1990915258
- Cited by
- 331
- References
- 1
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Umetani, N., Kim, J., Hiramatsu, S., Reber, H. A., Hines, O. J., Bilchik, A. J., & Hoon, D. S. B. (2006). Increased Integrity of Free Circulating DNA in Sera of Patients with Colorectal or Periampullary Cancer: Direct Quantitative PCR for ALU Repeats. Clinical Chemistry. https://doi.org/10.1373/clinchem.2006.068577
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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