The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Direct Serum Assay for MicroRNA-21 Concentrations in Early and Advanced Breast Cancer
Abstract
BACKGROUND: MicroRNAs (miRs) are a class of small noncoding RNAs whose expression changes have been associated with cancer development and progression. Current techniques to isolate miRs for expression analysis from blood are inefficient. We developed a reverse-transcription quantitative real-time PCR (RT-qPCR) assay for direct detection of circulating miRs in serum. We hypothesized that serum concentrations of miR-21, a biomarker increased in breast tumors, would correlate with the presence and extent of breast cancer. METHODS: The RT-qPCR applied directly in serum (RT-qPCR-DS) assay for circulating miR-21 was tested in sera from 102 patients with different stages of breast cancer and 20 healthy female donors. RESULTS: The assay was sensitive for detection of miR-21 in 0.625 μL of serum from breast cancer patients. For differentiation of samples from patients with locoregional breast cancer from those from healthy donors, the odds ratio was 1.796 and the area under the curve was 0.721. In a multivariate analysis that included standard clinicopathologic prognostic factors, high circulating miR-21 concentrations correlated significantly (P < 0.001) with visceral metastasis. CONCLUSIONS: A novel RT-qPCR-DS can improve the efficiency of miR assessment. Use of this assay to detect circulating miR-21 has diagnostic and prognostic potential in breast cancer.
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Bartel (2004) · doi:10.1016/s0092-8674(04)00045-5 · Cited by 34,714
The functions of animal microRNAs
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Cited by
Clinical relevance of circulating cell-free microRNAs in cancer
Schwarzenbach, H., Nishida, N., Calin, G. A., & Pantel, K. (2014) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2014.5 · Cited by 969
The role of microRNAs in human diseases
Tüfekci, K. U., Öner, M. G., Meuwissen, R. L. J., & Genç, Ş. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-748-8_3 · Cited by 566
Circulating miRNAs: Roles in cancer diagnosis, prognosis and therapy
Cheng, G. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.09.001 · Cited by 334
Metastatic breast cancer: the potential of miRNA for diagnosis and treatment monitoring
McGuire, A., Brown, J. A. L., & Kerin, M. J. (2015) · Cancer and Metastasis Reviews · doi:10.1007/s10555-015-9551-7 · Cited by 329
Serum Circulating microRNA Profiling for Identification of Potential Breast Cancer Biomarkers
Mar-Aguilar, F., Mendoza-Ramírez, J. A., Malagón-Santiago, I., Espino-Silva, P. K., Santuario-Facio, S. K., Ruiz-Flores, P., Rodríguez-Padilla, C., & Reséndez-Pérez, D. (2013) · Disease Markers · doi:10.1155/2013/259454 · Cited by 280
Metadata
- Authors
- Asaga, S., Kuo, C., Nguyen, T., Terpenning, M., Giuliano, A. E., & Hoon, D. S.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-01-01
- Volume
- 57
- Issue
- 1
- Pages
- 84-91
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2127165438
- Cited by
- 451
- References
- 7
- FWCI
- 8.67
- Type
- journal article
- Language
- en
- Open access
- closed
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- Asaga, S., Kuo, C., Nguyen, T., Terpenning, M., Giuliano, A. E., & Hoon, D. S. (2011). Direct Serum Assay for MicroRNA-21 Concentrations in Early and Advanced Breast Cancer. Clinical Chemistry. https://doi.org/10.1373/clinchem.2010.151845
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