Single-Strand Conformational Polymorphism Analysis: Basic Principles and Routine Practice
Abstract
Single-strand conformational polymorphism (SSCP) analysis is a simple and sensitive technique for mutation detection and genotyping. The principle of SSCP analysis is based on the fact that single-stranded DNA has a defined conformation. Altered conformation due to a single base change in the sequence can cause single-stranded DNA to migrate differently under nondenaturing electrophoresis conditions. Therefore wild-type and mutant DNA samples display different band patterns. SSCP analysis involves the following four steps: (1) polymerase chain reaction (PCR) amplification of DNA sequence of interest; (2) denaturation of double-stranded PCR products; (3) cooling of the denatured DNA (single-stranded) to maximize self-annealing; (4) detection of mobility difference of the single-stranded DNAs by electrophoresis under non-denaturing conditions. Several methods have been developed to visualize the SSCP mobility shifts. These include the incorporation of radioisotope labeling, silver staining, fluorescent dye-labeled PCR primers, and more recently, capillary-based electrophoresis. Silver staining is simple, rapid, and cost-effective, and can be routinely performed in clinical laboratories. The use of SSCP analysis to discover and genotype single-nucleotide polymorphisms (SNPs) has been widely applied to the genetics of hypertension, including both monogenic (e.g., Liddle's syndrome) and polygenic disorders (e.g., essential hypertension).
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Altered conformation due to a single base change in the DNA can cause different band patterns on non-denaturing gels which separate mutant and wild-type DNA.
“Altered conformation due to a single base change in the DNA can cause different band patterns on non-denaturing gels which separate mutant and wild-type DNA (Zhu, 2005).”
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Cites
Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K., & Sekiya, T. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.8.2766 · Cited by 3,589 · see citing works
Mashiyama, S., Kayama, T., Katakura, R., Yoshimoto, T., Murakami, Y., Sekiya, T., & Hayashi, K. (1991) · Springer eBooks · doi:10.1007/978-4-431-68150-2_41 · Cited by 538 · see citing works
Diagnostic strategies in CADASIL
Markus, H. S., Martin, R. J., Simpson, M. A., Dong, Y. B., Ali, N., Crosby, A. H., & Powell, J. F. (2002) · Neurology · doi:10.1212/wnl.59.8.1134 · Cited by 335 · see citing works
Self-citationHongyo, T., Buzard, G. S., Calvert, R. J., & Weghorst, C. M. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.16.3637 · Cited by 324 · see citing works
Baker, E. H., Dong, Y., Sagnella, G. A., Rothwell, M. J., Onipinla, A., Markandu, N., Cappuccio, F. P., Cook, D. G., Persu, A., Corvol, P. L., et al. (1998) · The Lancet · doi:10.1016/s0140-6736(97)07306-6 · Cited by 243 · see citing works
Self-citationGenetic Analysis of the β Subunit of the Epithelial Na + Channel in Essential Hypertension
Persu, A., Barbry, P., Bassilana, F., et al. (1998) · Hypertension · doi:10.1161/01.hyp.32.1.129 · Cited by 122 · see citing works
Makino, R., Yazyu, H., Kishimoto, Y., et al. (1992) · Genome Research · doi:10.1101/gr.2.1.10 · Cited by 104 · see citing works
Genotype–phenotype analysis of a newly discovered family with Liddleʼs syndrome
Jeunemaı̂tre, X., Bassilana, F., Persu, A., et al. (1997) · Journal of Hypertension · doi:10.1097/00004872-199715100-00007 · Cited by 96 · see citing works
Inazuka, M., Wenz, H. M., Sakabe, M., et al. (1997) · Genome Research · doi:10.1101/gr.7.11.1094 · Cited by 95 · see citing works
Electrokinetic transport of red blood cells in microcapillaries
Minerick, A. R., Ostafin, A., & Chang, H. (2002) · Electrophoresis · doi:10.1002/1522-2683(200207)23:14 · Cited by 91 · see citing works
Yield of Screening for CADASIL Mutations in Lacunar Stroke and Leukoaraiosis
Dong, Y., Hassan, A., Zhang, Z., Huber, D., Dalageorgou, C., & Markus, H. S. (2003) · Stroke · doi:10.1161/01.str.0000048162.16852.88 · Cited by 89 · see citing works
Self-citationAndersen, P. S., Jespersgaard, C., Vuust, J., et al. (2003) · Human Mutation · doi:10.1002/humu.10169 · Cited by 84 · see citing works
Baroni, M. G., Arca, M., Sentinelli, F., Buzzetti, R., Capici, F., Lovari, S., Vitale, M., Romeo, S., & Mario, U. D. (2001) · Diabetologia · doi:10.1007/s001250051628 · Cited by 66 · see citing works
Zhu, H., Sagnella, G. A., Dong, Y., Miller, M. A., Onipinla, A. K., Markandu, N. D., & MacGregor, G. A. (2003) · Journal of Hypertension · doi:10.1097/00004872-200301000-00018 · Cited by 55 · see citing works
Self-citationAndersen, P. S., Jespersgaard, C., Vuust, J., et al. (2003) · Human Mutation · doi:10.1002/humu.10161 · Cited by 51 · see citing works
Methylation, expression, and mutation analysis of the cell cycle control genes in human brain tumors
Yin, D., Xie, D., Hofmann, W., Miller, C. W., Black, K. L., & Koeffler, H. P. (2002) · Oncogene · doi:10.1038/sj.onc.1206031 · Cited by 47 · see citing works
T594M and G442V polymorphisms of the sodium channel β subunit and hypertension in a black population
Dong, Y., Zhu, H., Baker, E. H., et al. (2001) · Journal of Human Hypertension · doi:10.1038/sj.jhh.1001182 · Cited by 38 · see citing works
Self-citationKlumb, C. E., Furtado, D. R., de Resende, L. M. M., et al. (2003) · European Journal Of Haematology · doi:10.1034/j.1600-0609.2003.00094.x · Cited by 28 · see citing works
Wang, H., Chu, W. S., Das, S. K., Zheng, Z., Hasstedt, S. J., & Elbein, S. C. (2003) · Molecular Genetics and Metabolism · doi:10.1016/s1096-7192(03)00096-9 · Cited by 20 · see citing works
Bettinaglio, P., Galbusera, A., Caprioli, J., et al. (2002) · Clinical Biochemistry · doi:10.1016/s0009-9120(02)00308-9 · Cited by 14 · see citing works
Detection and characterization of new mutations in the human angiotensinogen gene (AGT)
Hixson, J. E., & Powers, P. K. (1995) · Human Genetics · doi:10.1007/bf00214197 · Cited by 12 · see citing works
Gupta, A. (2003) · Molecular Pathology · doi:10.1136/mp.56.4.237 · Cited by 11 · see citing works
Baroni, M. G., Sentinelli, F., Massa, O., Romeo, S., Colombo, C., Mario, U. D., & Barbetti, F. (2001) · Journal of Molecular Medicine · doi:10.1007/s001090100220 · Cited by 7 · see citing works
Dong, Y., Baker, E. H., Sagnella, G. A., et al. (1998) · Biochemical Society Transactions · doi:10.1042/bst026s393 · Cited by 3 · see citing works
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Schreiber, L., Nader-Nieto, A. C., Schönhals, E. M., Walkemeier, B., & Gebhardt, C. (2014) · G3 Genes|Genomes|Genetics · doi:10.1534/g3.114.012377 · Cited by 81 · see citing works
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18 · see citing works
“Altered conformation due to a single base change in the DNA can cause different band patterns on non-denaturing gels which separate mutant and wild-type DNA (Zhu, 2005).”
Influential
- Authors
- Dong, Y., & Zhu, H.
- Affiliations
- Augusta University (US)
- Venue
- Hypertension
- Publisher
- Humana Press
- Published
- 2005-07-21
- Volume
- 108
- Pages
- 149-158
- OpenAlex
- W133571668
- Cited by
- 44
- References
- 24
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 5 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 5 · 2018: 1 · 2017: 5 · 2016: 2 · 2014: 2 · 2013: 1
- FWCI
- 5.99
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Humana Press eBooks (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomics and Rare Diseases
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- single-strand conformation polymorphism, mutation detection, genotyping, single nucleotide polymorphisms, silver staining, self-annealing, capillary electrophoresis, hypertension genetics, Liddle syndrome, essential hypertension
- MeSH terms
- DNA, Electrophoresis, Polymorphism, Single-Stranded Conformational
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