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Proteomic Analysis by Multidimensional Protein Identification Technology
Abstract
Multidimensional chromatography coupled to mass spectrometry is an emerging technique for the analysis of complex protein mixtures. One approach in this general category, multidimensional protein identification technology (MudPIT), couples biphasic or triphasic microcapillary columns to high-performance liquid chromatography, tandem mass spectrometry, and database searching. The integration of each of these components is critical to the implementation of MudPIT in a laboratory. MudPIT can be used for the analysis of complex peptide mixtures generated from biofluids, tissues, cells, organelles, or protein complexes. The information described in this chapter will provide researchers with details for sample preparation, column assembly, and chromatography parameters for complex peptide mixture analysis.
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Cited by
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Hrecka, K., Hao, C., Gierszewska, M., Swanson, S. K., Kesik-Brodacka, M., Srivastava, S., Florens, L., Washburn, M. P., & Skowronski, J. (2011) · Nature · doi:10.1038/nature10195 · Cited by 1,200
Self-citationAneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast
Pavelka, N., Rancati, G., Zhu, J., Bradford, W. D., Saraf, A., Florens, L., Sanderson, B. W., Hattem, G. L., & Li, R. (2010) · Nature · doi:10.1038/nature09529 · Cited by 577
Self-citationPaoletti, A. C., Parmely, T. J., Tomomori-Sato, C., Sato, S., Zhu, D., Conaway, R. C., Conaway, J. W., Florens, L., & Washburn, M. P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606379103 · Cited by 538
Self-citationCytosolic proteostasis through importing of misfolded proteins into mitochondria
Ruan, L., Zhou, C., Jin, E., Kucharavy, A., Zhang, Y., Wen, Z., Florens, L., & Li, R. (2017) · Nature · doi:10.1038/nature21695 · Cited by 474
Self-citationLitovchick, L., Sadasivam, S., Florens, L., Zhu, X., Swanson, S. K., Velmurugan, S., Chen, R., Washburn, M. P., Liu, X. S., & DeCaprio, J. A. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.04.015 · Cited by 396
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Metadata
- Authors
- Florens, L., & Washburn, M. P.
- Venue
- New and Emerging Proteomic Techniques
- Publisher
- Humana Press
- Published
- 2006-06-24
- Volume
- 328
- Pages
- 159-176
- OpenAlex
- W2413189460
- Cited by
- 240
- References
- 0
- FWCI
- 139.44
- Type
- book chapter
- Language
- en
- Open access
- closed
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