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Cited by 39Neuropeptides and Animal PhysiologyRegulation of Appetite and ObesityHypothalamic control of reproductive hormones

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Distribution of NPY Y5-Like Immunoreactivity in the Rat Brain

Morin, S. Michelle, Gehlert, Donald R.·2006·Journal of Molecular Neuroscience
DOI 10.1385/jmn:29:2:109
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Abstract

The pharmacology and brain mRNA distribution of the neuropeptide Y (NPY) rat Y5 (rY5) receptor has led to the hypothesis that this receptor might mediate the hypothalamic feeding response to NPY in addition to many other physiologic functions. However, through the use of autoradiographic techniques, only very low levels of Y5-like immunoreactive (Y5-ir) binding are detected in the rat brain. To localize the Y5 protein in the rat brain, polyclonal antibodies were raised to the carboxyl terminus of the rY5 receptor. The resulting antisera were affinity purified and characterized by specific binding to HEK293 cells that had been stably transfected with the rY5 receptor. Utilizing immunohistochemical techniques, we found a discrete pattern of Y5-ir in the rat brain. In initial studies, very low levels of Y5-ir were detected, and TSA amplification was required to visualize the staining. Areas with the highest levels of expression include the piriform cortex, supraoptic nucleus, and hippocampus. Areas with moderate levels of expression include the lateral septum, amygdala, arcuate nucleus, paraventricular hypothalamic nucleus, locus coeruleus, and cerebellum. With several exceptions, this pattern of distribution is consistent with earlier reports of rY5 mRNA and receptor protein expression.

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Metadata

Authors
Morin, S. M., & Gehlert, D. R.
Affiliations
Eli Lilly (United States) (US)
Corresponding author
Gehlert, Donald R.
Venue
Journal of Molecular Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2006
Volume
29
Issue
2
Pages
109-114
ISSN
0895-8696 (ISSN-L)
DOI
10.1385/jmn:29:2:109
OpenAlex
W2057882002
Cited by
39
References
29
Citations by year
2025: 1 · 2023: 1 · 2021: 3 · 2020: 1 · 2019: 3 · 2017: 2 · 2016: 3 · 2014: 2 · 2013: 4 · 2012: 6
FWCI
0.63
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Molecular Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords
neuropeptide Y, Y5 receptor, rat brain, immunoreactivity, supraoptic nucleus, piriform cortex, hippocampus, arcuate nucleus, paraventricular nucleus, polyclonal antibodies, receptor localization
MeSH terms
Animals, Brain, Cell Line, Cricetulus, Cricetinae, Humans, Male, Neuropeptide Y, CHO Cells, Rats, Sprague-Dawley, Receptors, Neuropeptide Y, Protein Isoforms, Rats

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