Skin development in bony fish with particular emphasis on collagen deposition in the dermis of the zebrafish (Danio rerio)
Open accessAbstract
The first part of this article is a review of the current status of knowledge of the fish skin, with particular attention to its development. In the second part we present original results obtained in zebrafish (Danio rerio), with particular emphasis on the deposition and organisation of the dermal collagenous stroma. Using a series of zebrafish specimens aged between 15 hours postfertilization (hpf) and 4.5 years old, we have combined Transmission Electron Microscopy (TEM) observations and in situ hybridisation using type I collagen a2 chain (Col1a2) probe. Collagen fibrils, with a diameter of 22 nm, appear first in an acellular subepidermal space at 24 hpf, are first all oriented in the same direction, and form the primary dermal stroma. Subsequently, three events occur. (1) From 5-7 days pf (dpf) onwards the collagen fibrils self-organise into several lamellae arranged in a plywood-like structure, starting in the upper layers and progressing throughout the entire thickness of the dermis. (2) At 20-26 dpf, fibroblasts of unknown origin progressively invade the acellular collagenous stroma, some of them accumulating below the epidermis. (3) Concomitant with the invasion of fibroblasts, the collagen fibrils increase progressively in diameter to reach 160 nm towards the end of the fish life. In situ hybridisation experiments reveal that, between 24 and 48 hpf, the collagen matrix is produced by the epidermis only. From 72 hpf to 20-26 dpf, both the basal epidermal cells and the dermal cells bordering the deep region of the dermis are involved in the production of collagen. When the fibroblasts invade the plywood-like structure, the epidermal cells progressively cease to synthesise collagen, which from this point is produced only by the fibroblasts. This suggests that the fibroblasts secrete a still unidentified signalling molecule that downregulates collagen production by the epidermis.
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The iridophores of the skin are a dermal-hypodermal structure normally intimately connected to surrounding xanthophores and lie just above the trunk skeletal muscle.
“The iridophores of the skin are a dermal-hypodermal structure which are normally intimately connected to the surrounding xanthophores and lie just above the trunk skeletal muscle”
From Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis (2008) · cites this paper as Le Guellec et al., 2004
In “An overview of the current knowledge of fish skin” (page unavailable), the paper places iridophores and xanthophores in the hypodermis, between the dermis and underlying muscle. It does not say that iridophores are normally intimately connected to xanthophores, or specifically describe iridophores as a dermal-hypodermal structure. (LLM, conf=0.84)
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- HAY, E.D. and REVEL, J.P. (1969) Fine structure of the developing avian cornea. In: Monographs in Developmental Biology (Wolski, A. & Chen P.S., eds) vol. 1: 1-144. Karger AG., Basel, Switzerland.
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- SIRE, J.-Y. and AKIMENKO, M.-A. (2004). Scale development in fish: a review with description of sonic hedgehog (shh) expression in the zebrafish (Danio rerio). Int. J. Dev. Biol. 48: 233-247.
- VEIS, A. and GEORGE, A. (1994) Fundamentals of interstitial collagen self- assembly. In: Extracellular Mtrix Assembly and Function (Yurchenco, P.D., Birk, D.E. and Mecham, R.P., eds), pp. 15-45. Academic Press, San Diego.
- WEISS, P. and FERRIS, W. (1954) Electron-microscopic study of the texture of the basement membrane of larval amphibian skin. Proc. Nat. Acad. Sci. U.S.A., 40: 528-540.
- WHITEAR, M. (1986) The skin of fishes including cyclostomes. Epidermis. Dermis. In: Biology of the Integument. 2. Vertebrates (Bereiter-Hahn, J., Matoltzy, A.G. and Richards, S., eds), pp. 8-64. Springer-Verlag, Heidelberg.
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- Authors
- Le Guellec, D., Morvan-Dubois, G., & Sire, J.-Y.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Université Pierre-et-Marie-Curie (FR)
- Venue
- The International Journal of Developmental Biology
- Publisher
- EHU Press
- Published
- 2004
- Volume
- 48
- Issue
- 2-3
- Pages
- 217-231
- ISSN
- 0214-6282 (ISSN-L)
- OpenAlex
- W2083457899
- Cited by
- 286
- References
- 0
- Citations by year
- 2026: 11 · 2025: 8 · 2024: 16 · 2023: 17 · 2022: 12 · 2021: 12 · 2020: 29 · 2019: 29 · 2018: 15 · 2017: 7 · 2016: 9 · 2015: 13 · 2014: 14 · 2013: 12 · 2012: 10
- FWCI
- 0.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The International Journal of Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Ligue Contre le Cancer
- Universiteit Gent
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- zebrafish, skin collagen, collagen deposition, type I collagen, COL1A2, fibroblast invasion
- MeSH terms
- Animals, Cell Movement, Collagen, Embryo, Nonmammalian, Epidermis, Extracellular Matrix, Fibroblasts, Larva, Models, Biological, RNA, Messenger, Skin, Time Factors, Zebrafish, Down-Regulation, In Situ Hybridization, Gene Expression Regulation, Developmental, Dermis, Collagen Type I
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