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Advanced methods of microscope control using μManager software

Open access
D. Edelstein, Arthur, A. Tsuchida, Mark, Amodaj, Nenad, Pinkard, Henry, D. Vale, Ronald, Stuurman, Nico·2014·Journal of Biological Methods

Abstract

µManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, µManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced µManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.

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Metadata

Authors
D. Edelstein, A., A. Tsuchida, M., Amodaj, N., Pinkard, H., D. Vale, R., & Stuurman, N.
Venue
Journal of Biological Methods
Published
2014-11-07
Volume
1
Issue
2
Pages
1
ISSN
2326-9901 (ISSN-L)
OpenAlex
W2137753785
Cited by
1,909
References
5
FWCI
41.35
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Advanced methods of microscope control using μManager software. https://app.cochat.ai/papers/10.14440/jbm.2014.36
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D. Edelstein, A., A. Tsuchida, M., Amodaj, N., Pinkard, H., D. Vale, R., & Stuurman, N. (2014). Advanced methods of microscope control using μManager software. Journal of Biological Methods. https://doi.org/10.14440/jbm.2014.36

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