Sphaeranthus indicus Flower Derived Constituents Exhibits Synergistic Effect against Acetylcholinesterase and Possess Potential Antiamnestic Activity
Abstract
Objective: The present investigation was aimed at discovery of novel acetylcholinesterase (AChE) inhibitors.Methods: In vitro AchE inhibitory activity of various extracts of Sphaeranthus indicus flower heads was carried out. The petroleum ether fraction of S. indicus flowers (SIPE) exhibited significant activity. The fraction was found to be rich in sesquiterpene lactone content possibly responsible for in vitro AChE inhibition. In further study, the antiamnesic activities of SIPE in mice on the learning and memory impairments induced by scopolamine (1.0 mg/kg, i.p.) were examined.Results: SIPE (10 mg/kg, p.o.) administration significantly reversed cognitive impairments in mice by passive avoidance test (P < 0.05). It also reduced escape latencies in training trials and prolonged swimming times in the target quadrant during the probe trial in the water maze task (P < 0.05). These results indicated that S. indicus due to its sesquiterpene lactones have anti-cholinesterase activity. A major sesquiterpene lactone, 7-hydroxy frullanolide along with other constituents were isolated from SIPE and evaluated for AchE inhibitory activity. Negative results were obtained in case of isolated compounds.Conclusion: Synergistic effect between constituents of SIPE was confirmed to have anti-amnesic activities that may be useful for cognitive impairment treatment.
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Petroleum ether fraction of Sphaeranthus indicus was loaded with sesquiterpene lactones (about 80%), accounting for its anti-AChE activity.
“Petroleum ether fraction was found to be loaded with SLs (about 80%), accounting for its anti-AChE activity.”
From Acetylcholinesterase Inhibitory Potential of Various Sesquiterpene Analogues for Alzheimer’s Disease Therapy (2021) · cites this paper as [125]
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“sesquiterpene lactones act in a synergistic way in AChE related disorders [42].”
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“Additionally, a sesquiterpene lactone is responsible for anticholinesterase activity (Patel and Amin 2012).”
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- Authors
- Patel, M. B., & Amin, D.
- Affiliations
- Atmiya University (IN)
- Venue
- Journal of Complementary and Integrative Medicine
- Publisher
- Walter de Gruyter GmbH
- Published
- 2012-09-24
- Volume
- 9
- Issue
- 1
- Pages
- Article 23-Article 23
- ISSN
- 1553-3840 (ISSN-L)
- OpenAlex
- W2008607234
- Cited by
- 17
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Complementary and Integrative Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Keywords
- Sphaeranthus indicus, acetylcholinesterase inhibition, sesquiterpene lactones, synergistic effect, scopolamine-induced cognitive impairment, memory impairment, cognitive enhancement
- MeSH terms
- Administration, Oral, Animals, Cholinesterase Inhibitors, Dose-Response Relationship, Drug, Drug Administration Schedule, Drug Synergism, Learning, Memory, Plant Extracts, Plants, Medicinal, Scopolamine, Sesquiterpenes, Maze Learning, Asteraceae, Inhibitory Concentration 50, Flowers, Mice
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