Cited by 323Proteoglycans and glycosaminoglycans researchIntramuscular injections and effectsHydrogels: synthesis, properties, applications

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Recombinant human hyaluronidase (rHuPH20): an enabling platform for subcutaneous drug and fluid administration

Frost, Gregory I·2007·Expert Opinion on Drug Delivery

DOI 10.1517/17425247.4.4.427

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Abstract

The extracellular matrix is a significant barrier to the effective subcutaneous delivery of many drugs, limiting both pharmacokinetic parameters and injection volumes. The space outside adipocytes in the hypodermis is not a fluid, but rather a solid extracellular matrix of collageneous fibrils embedded within a glycosaminoglycan-rich viscoelastic gel that buffers convective forces. The extracellular matrix limits the volume of drug that can be injected at a single site, as well as the rate and amount that reach the vascular compartment. A fully human recombinant DNA-derived hyaluronidase enzyme (rHuPH20) has been developed to leverage the historical efficacy of animal testes extract-derived spreading factors to reversibly modify the hypodermis, in light of discovery of the human hyaluronidase gene family. The application of this technology to increase both injection volumes and bioavailability from subcutaneous injection may overcome some key limitations of this route of administration in multiple settings of care.

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Authors: Frost, G. I.
Affiliations: Halozyme Therapeutics (United States) (US)
Corresponding author: Frost, Gregory I
Venue: Expert Opinion on Drug Delivery
Publisher: Informa UK Limited
Published: 2007-07
Volume: 4
Issue: 4
Pages: 427-440
ISSN: 1742-5247 (ISSN-L)
1744-7593
DOI: 10.1517/17425247.4.4.427
OpenAlex: W2158548553
Cited by: 323
References: 66
Citations by year: 2026: 25 · 2025: 18 · 2024: 16 · 2023: 23 · 2022: 15 · 2021: 27 · 2020: 18 · 2019: 8 · 2018: 27 · 2017: 18 · 2016: 19 · 2015: 17 · 2014: 28 · 2013: 12 · 2012: 23
FWCI: 0.45
Type: journal article
Language: en
Open access: closed
Locations: Expert Opinion on Drug Delivery (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
Health Sciences › Medicine › Emergency Medicine › Intramuscular injections and effects
Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Keywords: recombinant human hyaluronidase, rHuPH20, subcutaneous drug delivery, extracellular matrix, hyaluronic acid, subcutaneous tissue, injection volume, bioavailability, hyaluronidase, glycosaminoglycans, collagen, pharmacokinetics
MeSH terms: Amino Acid Sequence, Animals, Extracellular Matrix, Humans, Hyaluronoglucosaminidase, Injections, Subcutaneous, Molecular Sequence Data, Recombinant Proteins, Solubility, Protein Engineering, Cell Adhesion Molecules, Dermis

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Recombinant human hyaluronidase (rHuPH20): an enabling platform for subcutaneous drug and fluid administration

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