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Cited by 323Proteoglycans and glycosaminoglycans researchIntramuscular injections and effectsHydrogels: synthesis, properties, applications

The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.

Recombinant human hyaluronidase (rHuPH20): an enabling platform for subcutaneous drug and fluid administration

Frost, Gregory I·2007·Expert Opinion on Drug Delivery
DOI 10.1517/17425247.4.4.427
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Abstract

The extracellular matrix is a significant barrier to the effective subcutaneous delivery of many drugs, limiting both pharmacokinetic parameters and injection volumes. The space outside adipocytes in the hypodermis is not a fluid, but rather a solid extracellular matrix of collageneous fibrils embedded within a glycosaminoglycan-rich viscoelastic gel that buffers convective forces. The extracellular matrix limits the volume of drug that can be injected at a single site, as well as the rate and amount that reach the vascular compartment. A fully human recombinant DNA-derived hyaluronidase enzyme (rHuPH20) has been developed to leverage the historical efficacy of animal testes extract-derived spreading factors to reversibly modify the hypodermis, in light of discovery of the human hyaluronidase gene family. The application of this technology to increase both injection volumes and bioavailability from subcutaneous injection may overcome some key limitations of this route of administration in multiple settings of care.

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Crossref · 10.1517/17425247.4.4.427

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Citations

Cites

Research works printed but not linked: 18

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Metadata

Authors
Frost, G. I.
Affiliations
Halozyme Therapeutics (United States) (US)
Corresponding author
Frost, Gregory I
Venue
Expert Opinion on Drug Delivery
Publisher
Informa UK Limited
Published
2007-07
Volume
4
Issue
4
Pages
427-440
ISSN
  • 1742-5247 (ISSN-L)
  • 1744-7593
DOI
10.1517/17425247.4.4.427
OpenAlex
W2158548553
Cited by
323
References
66
Citations by year
2026: 25 · 2025: 18 · 2024: 16 · 2023: 23 · 2022: 15 · 2021: 27 · 2020: 18 · 2019: 8 · 2018: 27 · 2017: 18 · 2016: 19 · 2015: 17 · 2014: 28 · 2013: 12 · 2012: 23
FWCI
0.45
Type
journal article
Language
en
Open access
closed
Locations
  • Expert Opinion on Drug Delivery (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Health Sciences › Medicine › Emergency Medicine › Intramuscular injections and effects
  • Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Keywords
recombinant human hyaluronidase, rHuPH20, subcutaneous drug delivery, extracellular matrix, hyaluronic acid, subcutaneous tissue, injection volume, bioavailability, hyaluronidase, glycosaminoglycans, collagen, pharmacokinetics
MeSH terms
Amino Acid Sequence, Animals, Extracellular Matrix, Humans, Hyaluronoglucosaminidase, Injections, Subcutaneous, Molecular Sequence Data, Recombinant Proteins, Solubility, Protein Engineering, Cell Adhesion Molecules, Dermis

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