The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
A Syntaxin 1, Gαo, and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization
Open accessAbstract
Presynaptic Ca V 2.2 (N-type) calcium channels are subject to modulation by interaction with syntaxin 1 and by a syntaxin 1-sensitive Gα O G-protein pathway. We used biochemical analysis of neuronal tissue lysates and a new quantitative test of colocalization by intensity correlation analysis at the giant calyx-type presynaptic terminal of the chick ciliary ganglion to explore the association of Ca V 2.2 with syntaxin 1 and Gα O. Ca V 2.2 could be localized by immunocytochemistry (antibody Ab571) in puncta on the release site aspect of the presynaptic terminal and close to synaptic vesicle clouds. Syntaxin 1 coimmunoprecipitated with Ca V 2.2 from chick brain and chick ciliary ganglia and was widely distributed on the presynaptic terminal membrane. A fraction of the total syntaxin 1 colocalized with the Ca V 2.2 puncta, whereas the bulk colocalized with MUNC18-1. Gα O,whether in its trimeric or monomeric state, did not coimmunoprecipitate with Ca V 2.2, MUNC18-1, or syntaxin 1. However, the G-protein exhibited a punctate staining on the calyx membrane with an intensity that varied in synchrony with that for both Ca channels and syntaxin 1 but only weakly with MUNC18-1. Thus, syntaxin 1 appears to be a component of two separate complexes at the presynaptic terminal, a minor one at the transmitter release site with Ca V 2.2 and Gα O, as well as in large clusters remote from the release site with MUNC18-1. These syntaxin 1 protein complexes may play distinct roles in presynaptic biology.
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Cites
Three-dimensional structure of the neuronal-Sec1–syntaxin 1a complex
Misura, K. M. S., Scheller, R. H., & Weis, W. I. (2000) · Nature · doi:10.1038/35006120 · Cited by 785
Modulation of ion-channel function by G-protein-coupled receptors
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A conformational switch in syntaxin during exocytosis: role of munc18
Dulubova, I. (1999) · The EMBO Journal · doi:10.1093/emboj/18.16.4372 · Cited by 742
Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin
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Isolation and culture of adult rat hippocampal neurons
Brewer, G. J. (1997) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(96)00136-7 · Cited by 447
Identification of a syntaxin-binding site on N-Type calcium channels
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Cited by
Calcium Channel Regulation and Presynaptic Plasticity
Catterall, W. A., & Few, A. P. (2008) · Neuron · doi:10.1016/j.neuron.2008.09.005 · Cited by 647
Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice
Lasagna-Reeves, C. A., Castillo-Carranza, D. L., Sengupta, U., Clos, A. L., Jackson, G. R., & Kayed, R. (2011) · Molecular Neurodegeneration · doi:10.1186/1750-1326-6-39 · Cited by 633
Identification of oligomers at early stages of tau aggregation in Alzheimer's disease
Lasagna‐Reeves, C. A., Castillo‐Carranza, D. L., Sengupta, U., Sarmiento, J., Troncoso, J., Jackson, G. R., & Kayed, R. (2012) · The FASEB Journal · doi:10.1096/fj.11-199851 · Cited by 527
Germline mutations in TMEM127 confer susceptibility to pheochromocytoma
Qin, Y., Yao, L., King, E. E., Buddavarapu, K., Lenci, R. E., Chocron, E. S., Lechleiter, J. D., Sass, M., Aronin, N., Schiavi, F., et al. (2010) · Nature Genetics · doi:10.1038/ng.533 · Cited by 379
Ca2+-Activated K+Channels: From Protein Complexes to Function
Berkefeld, H., Fakler, B., & Schulte, U. (2010) · Physiological Reviews · doi:10.1152/physrev.00049.2009 · Cited by 274
Metadata
- Authors
- Li, Q., Lau, A., Morris, T. J., Guo, L., Fordyce, C. B., & Stanley, E. F.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2004-04-21
- Volume
- 24
- Issue
- 16
- Pages
- 4070-4081
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W2083928750
- Cited by
- 708
- References
- 36
- FWCI
- 4.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Li, Q., Lau, A., Morris, T. J., Guo, L., Fordyce, C. B., & Stanley, E. F. (2004). A Syntaxin 1, Gαo, and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.0346-04.2004
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